Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

Sun, Dan; Wu, Rui; He, Xianjing; Wang, Shuang; Lin, Yun-cheng; Xu, Han; Wang, Yue-qiang; Guo, Tong; Wu, Guojun; Yang, Keli

doi:10.1016/s1671-2927(11)60160-0

Cited by 15 publications

(15 citation statements)

References 21 publications

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“…Oligonucleotide primers (F, 5'-ATC AAC CGA GAT TCC CCC AGT-3' and R, 5'-TCA CTA TCG GTC AGT CAG GAG-3') used were as described by Sun et al (2011 Enterobacteriaceae has been commonly associated with gastrointestinal inflammation in dogs (Xenoulis et al, 2008). All the E. coli isolates obtained in the present study were further confirmed using PCR targeting E16S gene.…”

Section: Confirmation Of Escherichia Coli By Pcrmentioning

confidence: 55%

Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Sharif¹,

Sreedevi²,

Chaitanya³

et al. 2017

Int. J. Livest. Res.

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Section: Confirmation Of Escherichia Coli By Pcrmentioning

confidence: 55%

Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Sharif¹,

Sreedevi²,

Chaitanya³

et al. 2017

Int. J. Livest. Res.

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“…There are few protocols with multiplex target genes that have been proposed to identify infectious agents of endometritis discharges (Sun et al. ) that are simpler, less expensive and faster than the use of several single PCR assays (Tramuta et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…) but few protocols with multiplex target genes have been proposed to identify infectious agents of endometritis discharges (Sun et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…There are many complications found in pathogenic factors of endometritis. It is suggested that pathogenic bacteria have an important role in the occurrence of endometritis (Sun et al 2011). Escherichia coli, Trueperella pyogenes (Arcanobacterium pyogenes), Fusobacterium necrophorum and Prevotella melaninogenicus are recognized uterine pathogens associated with uterine endometrial lesions (Sheldon and Dobson 2004;Williams et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Development of a Multiplex PCR for the Identification of Major Pathogenic Bacteria of Post‐Partum Endometritis in Dairy Cows

Aghamiri

Haghkhah

Ahmadi

et al. 2013

Reprod Domestic Animals

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Post-partum period has an important role in cows' breeding due to its effects on reproductive efficiency and subsequent pregnancy. Escherichia coli, Trueperella pyogenes (Arcanobacterium pyogenes), Fusobacterium necrophorum and Prevotella melaninogenicus are recognized as major pathogens associated with uterine endometrial lesions. The objective of this study was to identify these pathogens using the polymerase chain reaction (PCR) as a culture-independent sensitive method. A total of 172 cows were examined 25-35 days post-partum, and 128 cows were examined at 2 weeks later (39-49 days post-partum). Uterine discharges were collected by covered plastic infusion pipettes. The prevalence of endometritis was greater in the first examination than the second (35.5% vs. 16%). E. coli was detected in eight of the samples, T. pyogenes was detected in 13 of the samples and F. necrophorum was detected in 11 of the samples. There was no positive sample of P. melaninogenicus. Uterine contamination by T. pyogenes and F. necrophorum in the first examination was higher than the second examination. T. pyogenes affected as a tendency the prevalence of clinical endometritis in first examination. Primiparous cows showed 4.02 times higher odds of clinical endometritis compared with second-parity cows in first examination. A multiplex PCR protocol as a simple, less expensive, fast assay was introduced to identify E. coli, T. pyogenes and F. necrophorum.

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“…(1)(2)(3)(4)(5) Many studies conducted to identify major inflammatory diseases in dairy cattle have been reported and have described various techniques to enhance diagnosis and treatment. (6)(7)(8)(9) However, subclinical symptoms of inflammatory diseases in dairy cattle are difficult to detect due to the absence of any visible signs and often result in the emergence of more serious illnesses. Thus, early diagnosis is essential for the prevention and treatment of inflammatory diseases in dairy cattle.…”

Section: Introductionmentioning

confidence: 99%

A Polyclonal Antibody Against Recombinant Bovine Haptoglobin Expressed inEscherichia coli

Guo

Zhang²,

Li³

et al. 2013

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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The nucleotide sequence of the predicted immunodominant region of bovine haptoglobin (pirBoHp), without the signal peptide sequence, was synthesized based on the codon usage bias of Escherichia coli. The synthesized pirBoHp gene was cloned into the prokaryotic expression vector pET-32a (+), which contains a His-tag. The recombinant pirBoHp protein was successfully expressed in E. coli BL21 (DE3) cells. Western blot analysis showed that the purified recombinant pirBoHp protein could be recognized by an anti-His-tag monoclonal antibody. Further investigations indicated that a polyclonal antibody against the recombinant pirBoHp protein could recognize the α and β chains of native bovine haptoglobin in a pooled plasma sample from dairy cattle suffering from foot rot.

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Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

Cited by 15 publications

References 21 publications

Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Isolation and Identification of the Gut Microbiota of Healthy and Diarrhoeic Dogs in Andhra Pradesh, India

Development of a Multiplex PCR for the Identification of Major Pathogenic Bacteria of Post‐Partum Endometritis in Dairy Cows

A Polyclonal Antibody Against Recombinant Bovine Haptoglobin Expressed inEscherichia coli

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