Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time

Guillemi, Eliana Carolina; Ruybal, Paula; Lia, Verónica Viviana; González, Sergio; Lew, Sergio E.; Zimmer, Patricia Andrea; Arias, Ludmila Sol López; Rodrı́guez, José Luis; Rodriguez, Sonia Y.; Frutos, Roger; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana

doi:10.1016/j.meegid.2014.12.027

Cited by 16 publications

(19 citation statements)

References 61 publications

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“…As we have previously reported [ 11 ], there is a broad genetic diversity of A. marginale isolates in bovine host. Moreover, the chance of finding the same genotype in cattle samples is scarce (Fig.…”

Section: Discussionmentioning

confidence: 77%

“…Additionally we provide information regarding epidemiological analysis through genotyping of both isolates and comparing them with cattle genotypes reported to date. In this sense, the ST 69 found in both wild species resulted in a new variant consisting of seven alleles previously described in cattle from Argentina and other world regions [ 11 ]. Similarly, the two tandem repeats found through the MSP1a marker (repeat 13 and 27) were previously published for A. marginale isolates in cattle from Argentina, Mexico, Brazil and South Africa [ 12 ], but, as they had never been found together, their combination resulted in a novel genotype.…”

Section: Discussionmentioning

confidence: 99%

“…First, msp1α gene was amplified and sequenced for identifying the number and type of tandem repeats in the 5’region of the gene, according to the protocol described by [ 10 ]. Secondly, a multilocus sequence type (MLST) scheme based on the allelic polymorphism of seven A. marginale housekeeping genes ( dnaA, ftsZ, lipA, groEl, recA, secY and sucB ) was applied [ 11 ]. The nucleotide sequence of the primers employed and the resulting amplicon size are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…For automate MLST-DNA sequence editing and analysis, a custom-designed bioinformatic “Galaxy MLST-Pipeline” has been employed ( http://bioinformatica.inta.gov.ar/galaxy/ ) [ 11 ]. The information obtained from this pipeline was then used for inferring relatedness between allelic profiles through the eBURST algorithm [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

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Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines

Guillemi¹,

Fourniere²,

Orozco

et al. 2016

Parasites Vectors

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BackgroundAnaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.MethodsSamples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis.ResultsAnaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group.ConclusionsIn the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%