Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of
            <i>Leishmania</i>
            (
            <i>Viannia</i>
            ) Species

Oddone, Rolando; Schweynoch, Carola; Schönian, Gabriele; Sousa, Cíntia dos Santos de; Cupolillo, Elisa; Espinosa, Diego A.; Arévalo, Jorge; Noyes, Harry; Maurício, Isabel; Kuhls, Katrin

doi:10.1128/jcm.00645-09

Cited by 51 publications

(64 citation statements)

References 33 publications

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“…The best broad resolution was obtained with the enzyme ) braziliensis complex; however, discrimination between these two strains has been hindered because many parasites of this complex seem to have a composite genotype from both species. 11,12 Our finding reinforces the need to evaluate a larger sample of reference strains from both species.…”

supporting

confidence: 70%

Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing

Miranda

Samudio

González

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. A precise identification of Leishmania species involved in human infections has epidemiological and clinical importance. Herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing Leishmania species. After calmodulin amplification, the enzyme HaeIII yielded a clear distinction between reference strains of Leishmania mexicana, Leishmania amazonensis, Leishmania infantum, Leishmania lainsoni, and the rest of the Viannia reference species analyzed. The closely related Viannia species: Leishmania braziliensis, Leishmania panamensis, and Leishmania guyanensis, are separated in a subsequent digestion step with different restriction enzymes. We have developed a more accessible molecular protocol for Leishmania identification/typing based on the exploitation of part of the calmodulin gene. This methodology has the potential to become an additional tool for Leishmania species characterization and taxonomy.Leishmaniasis is a major neglected tropical disease affecting around 2 million people worldwide every year. 1 The infections tend to cluster among the poorest and most isolated increasing the socioeconomic burden upon these vulnerable populations. There are more than 20 species of Leishmania capable of infecting humans and each having distinct epidemiological/demographic patterns, and nearly all have a zoonotic transmission with one main sylvatic reservoir.2 In the absence of vaccines and other effective preventive measures, the control of leishmaniasis is currently based on accurate diagnosis and prompt treatment.A precise identification of the species has epidemiological, and particularly clinical importance, since the Leishmania species involved in human infections can influence the treatment decision and outcome.3 Furthermore, species identification is particularly relevant now that the disease appears to be currently underestimated and on the rise in several countries.

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supporting

confidence: 70%

Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing

Miranda

Samudio

González

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Cluster B (sustained by a bootstrap of 32%) corresponded to L. archibaldi taxon and three L. infantum from dogs. It has to be noticed that other studies have observed, using microsatellite method, small bootstrap for large clusters and important bootstrap values only for small clusters for L. braziliensis [45] and L. infantum [46].…”

Section: Resultsmentioning

confidence: 84%

Multifaceted Population Structure and Reproductive Strategy in Leishmania donovani Complex in One Sudanese Village

et al. 2011

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Leishmania species of the subgenus Leishmania and especially L. donovani are responsible for a large proportion of visceral leishmaniasis cases. The debate on the mode of reproduction and population structure of Leishmania parasites remains opened. It has been suggested that Leishmania parasites could alternate different modes of reproduction, more particularly clonality and frequent recombinations either between related individuals (endogamy) or between unrelated individuals (outcrossing) within strongly isolated subpopulations. To determine whether this assumption is generalized to other species, a population genetics analysis within Leishmania donovani complex strains was conducted within a single village. The results suggest that a mixed-mating reproduction system exists, an important heterogeneity of subsamples and the coexistence of several genetic entities in Sudanese L. donovani. Indeed, results showed significant genetic differentiation between the three taxa (L. donovani, L. infantum and L. archibaldi) and between the human or canine strains of such taxa, suggesting that there may be different imbricated transmission cycles involving either dogs or humans. Results also are in agreement with an almost strict specificity of L. donovani stricto sensu to human hosts. This empirical study demonstrates the complexity of population structure in the genus Leishmania and the need to pursue such kind of analyses at the smallest possible spatio-temporal and ecological scales.

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“…Strain IOC/L2493 was isolated from sample RB013 and was characterised as L. guyanensis based on all of the markers (Table I). However, after analysis of the L. (Viannia) strains with a battery of microsatellites, strain IOC/L2493 was included in the same cluster as Leishmania panamensis and L. shawi (Oddone et al 2009). …”

Section: Sensitivity Of Pcr Methods For the Diagnosis Of Leishmaniasimentioning

confidence: 99%

Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species

Graça

Volpini

Romero

et al. 2012

Mem. Inst. Oswaldo Cruz

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107

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Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania ( Viannia ) Species

Cited by 51 publications

References 33 publications

Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing

Calmodulin Polymerase Chain Reaction–Restriction Fragment Length Polymorphism for Leishmania Identification and Typing

Multifaceted Population Structure and Reproductive Strategy in Leishmania donovani Complex in One Sudanese Village

Development and validation of PCR-based assays for diagnosis of American cutaneous leishmaniasis and identificatio nof the parasite species

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