Development of a Multichannel Dialysis Microchip for Bioassay of Drug Efficacy and Retention

Abstract

Here, we developed a multichannel dialysis microchip having three sets of dialysis systems, which consisted of three independent circulation channels with a common micropump. Each dialysis system was composed of a pneumatic micropump (heart), dialysis unit (renal corpuscle), and cell culture chamber (drug target) as well as circulation and dialysate channels to mimic the circulation system. Small molecules were successfully separated in parallel from macromolecules at the dialysis components. Anticancer tests using this microchip showed results that considered both serum protein-binding nature and drug activity. In the anticancer bioassay, the multichannel chip showed similar reproducible and reliable results as those of the single-channel system but with higher throughput.

Design Artificial Stem Cell Nests for Stem Cell Niche in a Microfluidic Petri Dish Programmed by a Cell Phone

“…For example, gravity [12] or vacuum pump [13][14][15] lose precise flow control (liver-on-chip). Diaphragm pumps rely on deformable materials, which are imperfect optically and in biocompatibility (liver spheroids-on-a-chip for the insulin-regulated glucose model [16] or drug retention studies [17] ). Ferrofluid, [18] electroosmotic, [19] or electrochemical [20] pumps do not avoid the interference of ferrofluids, voltage, and current on the system.…”

An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue

“…As a continuation of previous work on explant culture by our team [14], first, we tried to integrate a NC-based membrane (160 μm thick) in the microfluidic device. Although this technological approach has been reported [55,56], the achievement of a perfect sealing remains very difficult to obtain [57] and we did not succeed in preventing the immersion of the explant. Therefore, we introduced a PC membrane (20 μm thick) according to the process reported by Chueh et al and it was very effective [58].…”

A Highly Programmable Magnetic Micropump

“…The stirred pump [12] has a sealing problem. Diaphragm pumps [13,14] need deformable materials that are imperfect in optical, biocompatibility. Ferrofluid, [15] electroosmotic, [16] or electrochemical [17] technology bring chemical interference and voltage interference.…”