Development of a monolithic silica extraction tip for the analysis of proteins

Miyazaki, Shota; Morisato, Kei; Ishizuka, N.; Minakuchi, Hiroyoshi; Shintani, Yukihiro; Furuno, Masahiro; Nakanishi, Koji

doi:10.1016/j.chroma.2004.03.025

Cited by 101 publications

(60 citation statements)

References 17 publications

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“…Alternatively, biomacromolecules can be immobilised at the surface of monoliths by adsorption [33,50]. Adsorption was also used to prepare Ti- [38,51] and Zr-coated [52] monoliths. Tables 1 and 2 provide an overview of the materials used by various researchers and the parameters that have been varied in the synthesis of silica monoliths by sol-gel reactions.…”

Section: Synthesismentioning

confidence: 99%

“…These samples need to be desalted and enriched prior to offline nanospray or MALDI analysis. To this end, a platform making use of pretreatment tips based on monolithic stationary phases with various surface chemistries corresponding to biomolecules of various characteristics (hydrophilic, hydrophobic, phosphorylated) was developed [51]. C18 tips were employed for the desalting and concentration of peptide samples.…”

Section: Other Applications Of Silica-based Monoliths In Proteomicsmentioning

confidence: 99%

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Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

Rieux

Niederländer

Verpoorte

et al. 2005

J of Separation Science

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In recent years, proteomics has been a subject of intense research. The complexity of proteomics samples has fostered technological developments. One of these addresses the need for more efficient and faster separations. Monolithic columns prepared from organic and silica monomers offer very efficient separations at low back-pressure. Silica-based monoliths have small-sized skeletons and a bimodal pore size distribution with microm-sized throughpores and nm-sized mesopores. This gives silica-based monoliths favourable properties for high-efficiency, fast separations, like a low-pressure drop across the column, fast mass transfer kinetics and a high binding capacity.

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Section: Synthesismentioning

confidence: 99%

Section: Other Applications Of Silica-based Monoliths In Proteomicsmentioning

confidence: 99%

Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

Rieux

Niederländer

Verpoorte

et al. 2005

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Furthermore, an imprinted porous polymer monolith fiber coated with home-made capillary glass 102 and poly(ST-DVB) carbon monolith fiber carbonized by heating the porous polymer 103 showed higher extraction efficiency than other commercially available SPME fibers. More recently, a boronate affinity monolith, 104,105 and C18 and titania-coated silica monolithic tips, 106 as sorbents for PMME, were used for the selective extraction of hydrophilic proteins and phosphopeptides from biological samples. Applications of these methacrylate monolithic tips were extended to the extraction of pindolol, metoprolol and local anesthetics from human plasma.…”

Section: ·3 In-tube Spme Techniquesmentioning

confidence: 99%

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

Kataoka

2011

ANAL. SCI.

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“…Similarly, materials for solid-phase extraction, SPE, purposes can be easily designed to exhibit superior permeability and extraction efficiency. 45,46 The propagation of light in a dielectrically disordered medium is modified by multiple scattering and interference. In strongly scattering media with a large refractive index variation on a length scale comparable to light wavelength, interference of multiply scattered light brings about various interest- ing phenomena such as photon localization.…”

Section: Monolithic Silica Columnsmentioning

confidence: 99%

Sol–Gel Process of Oxides Accompanied by Phase Separation

Nakanishi¹

2006

Bulletin of the Chemical Society of Japan

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A versatile sol-gel method for fabricating porous oxide materials with well-defined co-continuous macropores has been reviewed. The chemical instability, in many cases induced by polymerization of the network-forming components, triggers the formation of biphasic morphologies, followed by an irreversible freezing of the transient morphology by the sol-gel transition of the gelling phase. Upon removal of the non-gelling phase, an oxide framework comprising of controlled macropores can be obtained. The mesopore system of such macroporous materials can be further modified either by a physico-chemical treatment or a supramolecular templating technique. Pure silica and siloxane-based organicinorganic hybrids with a hierarchical pore system in monolithic form have been successfully applied to the novel type of separation medium for high performance liquid chromatography, HPLC. Additional topics are also described including recent advances in the 3D-analysis of the interfacial properties of macroporous systems, extended compositional variations in the network-forming phase, and emerging applications in areas of biochemistry.In the preparation of amorphous materials, the unstable or metastable state plays an important role in forming multi-phasic structures in the length scale longer than several nanometers. While in metastable states one often observes a formation of dispersed phases associated with thermally activated diffusion, unstable states are the starting points of various spontaneous morphology formation processes represented by the spinodal decomposition. In melt-quenching fabrication processes of oxide glasses, a pseudo one-phase material is initially formed by rapidly cooling a multi-component melt, and partially crystallized or phase-separated microstructures are controlled by subsequent reheating processes. The metastable glassy state is conveniently used to bring a system into a thermodynamically unstable region as well as to physically freeze the transient heterogeneous state at will. In a chemically polymerizing system, however, the polymerization and crosslinking reactions take place in the presence of a solvent. It is virtually impossible to physically freeze the reaction without influencing all the chemical interactions among the constituents. A continuous polymerization process is chemically equivalent to the continuous physical cooling with respect to the chemical interaction (mutual solubility) and material transport (mobility of the solutes). That is, the polymerizing system resulting in a sol-gel transition is a chemical analogue to a continuously cooled system resulting in a glass transition. If a process of developing heterogeneity in the system superposes a structurefreezing process, a competition will take place between the two processes. Various kinds of transient heterogeneous structures will be frozen in the resultant solidified materials.Phase-separation phenomena related to the heterogeneity formation in materials have been investigated first in metallic alloys and oxide glasses, follow...

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Development of a monolithic silica extraction tip for the analysis of proteins

Cited by 101 publications

References 17 publications

Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

Sol–Gel Process of Oxides Accompanied by Phase Separation

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