Development of a monoclonal-based enzyme-linked immunoassay for saxitoxin-induced protein

Smith, Donna S.; Kitts, David D.

doi:10.1016/0041-0101(94)90084-1

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Because of the potential health hazard to humans and animals, a quick, sensitive, and specific method is needed to determine the presence of toxins in shellfish. Several methods, including mouse bioassays (Hollingworth and Wekell, 1990), a receptor binding assay (Davio and Fontelo, 1984;Smith and Kitts;1994;Vieytes et al, 1993), tissue culture assays (Gallacher and Birkbeck, 1992;Jellett et al, 1995;Manger et al, 1993), and chemical methods have been used for the analysis of PSP toxins (Hollingworth and Wekell, 1990;Hungerford and Wekell, 1992;Luckas, 1992). However, most chemical methods are time consuming and require expensive instrumentation (Lawrence and Menard, 1991;Luckas, 1992;Mirocha et al, 1992;Oshima, 1995;Pleasance et al, 1992;Sullivan et al, 1988;Thibault et al, 1991); bioassays, however, are nonspecific and sometimes insensitive.…”

Section: Introductionmentioning

confidence: 99%

Screening of Paralytic Shellfish Posioning Toxins in Naturally Occurring Samples with Three Different Direct Competitive Enzyme-Linked Immunosorbent Assays

Chu

Hsu

Huang

et al. 1996

J. Agric. Food Chem.

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Among three direct competitive enzyme-linked immunosorbent assays (dc-ELISA) tested, a protocol using either anti-saxitoxin (STX)/STX-horseradish peroxidase (HRP) or anti-neo-STX/STX- HRP pairs was found to be most effective for screening paralytic shellfish poisoning (PSP) toxins in contaminated shellfish. An excellent agreement between the total PSP toxins (STX plus neo-STX levels) obtained by ELISA and by mouse assay was found. Analysis of 1540 naturally contaminated samples revealed that 211 (13.6%) samples were positive by the ELISA as compared with 175 (11.3%) by the mouse assays at the threshold level of 80 μg/100 g of sample. The distributions of the toxins at levels >40 and >80 μg/100 g in 1540 samples obtained by different ELISA formats are presented. The total PSP toxin levels (STX plus neo-STX) obtained from simultaneous analysis of both STX and neo-STX by ELISA are valid for accurate screening for the presence of PSP toxins in these samples and could eliminate as much as 80−85% of the samples needed to run a mouse assay. Keywords: ELISA; saxitoxin; neosaxitoxin; PSP

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Section: Introductionmentioning

confidence: 99%

Screening of Paralytic Shellfish Posioning Toxins in Naturally Occurring Samples with Three Different Direct Competitive Enzyme-Linked Immunosorbent Assays

Chu

Hsu

Huang

et al. 1996

J. Agric. Food Chem.

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“…Because of the potential health hazard, a quick, sensitive, and specific method is needed to determine the presence of toxins in shellfish. Several methods, including mouse bioassays (Hollingworth and Wekell, 1990), receptor binding assay (Davio and Fontelo, 1984;Smith and Kitts, 1994;Vieytes et al, 1993), tissue culture assays (Gallacher and Birkbeck, 1992;Jellett et al, 1992Jellett et al, , 1995Manger et al, 1993), and chemical methods, have been used for the analysis of PSP toxins (Hollingworth and Wekell, 1990). Details of these methods have recently been reviewed (Lucas, 1992;Hungerford and Wekell, 1992).…”

Section: Introductionmentioning

confidence: 99%

Direct Competitive Enzyme-Linked Immunosorbent Assay for Saxitoxin and Neosaxitoxin

Huang

Hsu

Chu

1996

J. Agric. Food Chem.

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Using specific ployclonal antibodies against saxitoxin (STX) or neosaxitoxin (neo-STX) in combination with either STX-horseradish peroxidase (HRP) or neo-STX-HRP, the efficacy of four different direct competitive enzyme-linked immunoassorbent assay (dc-ELISA) formats for the analysis of STX and neo-STX was evaluated. Concentrations causing 50% inhibition (ID 50 ) of binding of toxin-HRP conjugate to the antibodies by free toxins in various ELISAs were found in the range of 0.1-9.0 ng/mL. A dc-ELISA, using either anti-STX/STX-HRP or anti-neo-STX/neo-STX-HRP pairs (ID 50 values of 0.28 and 0.18 ng/mL for STX and neo-STX, respectively), was found to be most effective for the analysis of STX and neo-STX in naturally contaminated shellfish samples. The analytical recoveries of STX added to viscera extracts of butter clams, dungeness crab, tanner crab, and blue mussels in the range of 0.5-10 ng/mL -1 g -1 were found to be 88.1, 92.7, 92.2, and 93.5% with coefficients of variation of 3.9, 2.7, 9.7, and 2.8%, respectively. The detection limit for STX and neo-STX in these shellfish was around 0.2 ng/g of tissue. Gonyautoxins 1-4, but not the C group of PSP toxins, were also detectable in these two systems. Analysis of 154 naturally contaminated shellfish samples showed good correlation between the ELISA (STX plus neo-STX levels) and mouse assay data. The data reported here suggest that simultaneous analysis of both STX and neo-STX by ELISA is necessary for accurate determination of overall PSP toxin levels.

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Biotoxinas marinas: intoxicaciones por el consumo de moluscos bivalvos/Seafood toxins: poisoning by bivalve consumption

Martı́n

Garcı́a

Sanz

et al. 1996

Food sci. technol. int.

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Seafood toxins are becoming increasingly important as etiologic agents of foodborne diseases around the world. This is partly because of greater awareness of the potential problems of the paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrheic shellfish poisoning (DSP) and more recently, a new type of seafood toxicity, called amnesic shellfish poisoning (ASP). This review describes the molluskan shellfish and biotoxins implicated, the development of standardized methods for detecting and quantifying these toxins, the importance of the economic loss resulting from their presence and the establishment of regular chemical monitoring for marine toxins.

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Development of a monoclonal-based enzyme-linked immunoassay for saxitoxin-induced protein

Cited by 4 publications

References 12 publications

Screening of Paralytic Shellfish Posioning Toxins in Naturally Occurring Samples with Three Different Direct Competitive Enzyme-Linked Immunosorbent Assays

Screening of Paralytic Shellfish Posioning Toxins in Naturally Occurring Samples with Three Different Direct Competitive Enzyme-Linked Immunosorbent Assays

Direct Competitive Enzyme-Linked Immunosorbent Assay for Saxitoxin and Neosaxitoxin

Biotoxinas marinas: intoxicaciones por el consumo de moluscos bivalvos/Seafood toxins: poisoning by bivalve consumption

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