Development of a molecular CAPS marker for the self-incompatibility locus in Brassica napus and identification of different S alleles

Möhring, Silke; Horstmann, Verena; Esch, Elisabeth

doi:10.1111/j.1439-0523.2005.01081.x

Cited by 16 publications

(6 citation statements)

References 37 publications

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“…SI can be introduced into B. napus either by introgression from B. oleracea and B. rapa (MacKay 1977;Goring et al 1992) or by the resynthesis of B. napus from B. oleracea and B. rapa (Gowers 1989;Rahman 2005). The inheritance of SI in B. napus generated by introgression is controlled by a single dominant gene (Goring et al 1992), a single recessive gene (Möring et al 2005), or two genes (Yang et al 2001), while resynthesized B. napus shows a strong dominant SI phenotype (Rahman 2005;Ekuere et al 2004). In natural SC B. napus, self-compatibility is caused by independent mutations in the dominant S haplotype (Okamoto et al 2007); a latent S allele was identified and a suppressor system not linked to the S loci was deduced to mask the S phenotype (Ekuere et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus

Tan

et al. 2008

Euphytica

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A self-incompatible (SI) line, S-1300, and its maintainer 97-wen135, a self-compatible (SC) line, were used to study the inheritance of maintenance for self-incompatibility in B. napus. The ratio of SI plants to SC plants from S-1300 9 97-wen135 F 2 and (S-1300 9 97-wen135) 9 97-wen135 was 346:260 and 249:232, fitting the expected ratio of 9:7 and 1:1, respectively. Based on these observations, here we propose a genetic model in which two independent loci, S locus and S suppressor locus (sp), are predicted to control the inheritance of maintenance for selfincompatibility in B. napus. The genotypes of S-1300 and 97-wen135 are S 1300 S 1300 sp 1300 sp 1300 and S 135 S 135 sp 135 sp 135 , respectively. S 135 is dominant to S 1300 , but coexistence of sp 1300 and sp 135 fails to suppress S locus. Both S 1300 and S 135 can be suppressed by sp 135 , while sp 1300 can suppress S 135 but not S 1300 . The model contains two characteristics: that a dominant S locus exists in self-compatible B. napus, and that co-suppression will occur when sp loci are heterozygous. The model has been validated by the segregation of S phenotypes in the (S-1300 9 97-wen135) 9 S-1300, the progenies of SC S-1300 9 97-wen135 F 2 plants and DH population developed from S-1300 9 97-wen135 F 1 . This is the first study to report co-suppression of S suppressor loci in B. napus. The genetic model will be very useful for developing molecular markers linked to maintenance for selfincompatibility and for dissecting the mechanism of SI/SC in B. napus.

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Section: Introductionmentioning

confidence: 98%

Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus

Tan

et al. 2008

Euphytica

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“…The evidence collected in this study shows that SNP identification represents the best strategy for finding polymorphic markers for the construction of a C. arabica genetic map. CAPS markers have been applied as molecular tools in Arabidopsis (Konieczny and Ausubel 1993), Brassica napus (Möhring et al 2005), and Capsicum annuum L. (Minamiyama et al 2005). These studies report the efficient use of the CAPS method in plants to detect a clear and reproducible polymorphism, easily identified by using enzymes of frequent digestion.…”

Section: Discussionmentioning

confidence: 98%

Strategies to develop polymorphic markers for Coffea arabica L.

Zárate¹,

Cristancho²,

Moncada³

2009

Euphytica

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This study reports the implementation of three strategies for the development of genetic markers and their evaluation in both progenitors of an F2 population used for the construction of a genetic map of Coffea arabica. The strategies were Cleaved Amplified Polymorphic Sequences (CAPS), Single Strand Conformational Polymorphism (SSCP), and sequence analysis predicted Single Nucleotide Polymorphism (SNP). The methodologies were developed from different sequence sources: For CAPS, we used 25 COS sequences derived from Hedyotis spp. and 29 COSII sequences derived from Solanaceae and Rubiaceae species; for SSCP, we used 111 coffee EST sequences, 50 COSII sequences, and 10 C. arabica BAC end sequences. A low polymorphism was identified with the CAPS and SSCP methodologies. A total of 61 SNPs were identified in silico from 5,371 ESTs of coffee and from amplified, cloned, and sequenced COSII markers. Sixteen of these SNPs were validated with Luminex technology and 2 of them were polymorphic in C. arabica genotypes. This study highlights the difficulties of finding polymorphism in the species C. arabica where SNP identification seems to be the best strategy to search for polymorphic markers for this low diversity plant.

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“…Most studies reported that SI was under the control of a single S locus (Möhring et al 2005;Rahman 2005;Tang et al 2009;Zhang et al 2008a), while some studies suggested suppressor locus control of SI via an unknown mechanism (Ekuere et al 2004;Ma et al 2009;Yang et al 2001 …”

Section: Genetic Analysis Of Si In B Napusmentioning

confidence: 98%

“…Specific markers linked to SI in B. napus lines have been developed to identify different S alleles (Möhring et al 2005). SCAR (sequence-characterized amplified region) markers were developed from S-locus genes and co-segregated with SI lines and self-compatible lines, and its use in marker-assisted selection for the SI phenotype was effective Zhang et al 2008a), which is helpful for improving the maintainer line and accelerating the breeding of SI hybrids in B. napus.…”

Section: Potential Of Si In B Napus Breedingmentioning

confidence: 99%

Progress on characterization of self-incompatibility in Brassica napus L.

et al. 2011

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Self-incompatibility (SI) is a widespread mechanism in flowering plants that promotes outbreeding and thereby increases genetic diversity. Recognition specificity in Brassica is achieved by the interaction of the female determinant S-receptor kinase (SRK) and its ligand, the male determinant S-locus protein 11 (SP11). The interaction between SP11 and SRK triggers the signaling cascade in an S-haplotype-specific manner and results in the rejection of self-pollen, but the signal components involved are still not well characterized. S haplotypes are widespread in self-compatible amphidiploid B. napus, and the interaction of heterozygous S haplotypes causes the loss of SI. This review highlights the recent advances made towards understanding the genetic analysis, distribution, and evolution of S haplotypes, the signal factors, and the potential of SI in B. napus hybrid breeding program.

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Development of a molecular CAPS marker for the self-incompatibility locus in Brassica napus and identification of different S alleles

Cited by 16 publications

References 37 publications

Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus

Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus

Strategies to develop polymorphic markers for Coffea arabica L.

Progress on characterization of self-incompatibility in Brassica napus L.

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