Development of a modular system for detection of genetically modified organisms in food based on ligation-dependent probe amplification

Ehlert, Alexandra; Moreano, Francisco; Busch, Ulrich; Engel, Karl‐Heinz

doi:10.1007/s00217-007-0790-x

Cited by 26 publications

(16 citation statements)

References 34 publications

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“…Further detection of the amplicons is carried out by capillary electrophoresis. This technique has been used successfully for the screening of multiple products within the same reaction [99,103]. Ehlert et al [104] described a multiplex ligation-dependent probe amplification (MLPA) method for the detection of different tree nuts (peanut, cashew, pecan, pistachio, hazelnut, macadamia nut, almond, walnut, and Brazil nut) and sesame seeds with a LOD of 5 ppm.…”

Section: Pcr and Real-time Pcrmentioning

confidence: 99%

Recent Advances in the Detection of Allergens in Foods

Cruz

López-Calleja

Martı́n

et al. 2017

Methods in Molecular Biology

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Food allergy is a public health issue that has significantly increased worldwide in the past decade affecting consumers' quality of life and making increasing demands on health service resources. Despite recent advances in many areas of diagnosis and treatment, our general knowledge of the basic mechanisms of the disease remained limited, i.e., not at pace with the exponential number of new cases and the explosion of the new technologies. For sensitized individuals, the only effective way to prevent allergic reactions is the strict avoidance of the offending food. For this reason, a number of regulatory bodies in several countries have recognized the importance of providing information about the presence of food allergens by enacting laws, regulations, or standards for food labeling of "priority allergens." This has resulted in the need for the development of analytical methods for protection of food-allergic consumers that should be among others highly specific, sensitive, and not influenced by the presence of the food matrix components. Several analytical approaches target either the allergen itself or a corresponding allergen marker such as peptide fragment or gene segment and have been used in the detection and quantification of allergens in food products. In this short review, some of the conventional and new methods for the detection of allergens in food are listed and briefly discussed.

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Section: Pcr and Real-time Pcrmentioning

confidence: 99%

Recent Advances in the Detection of Allergens in Foods

Cruz

López-Calleja

Martı́n

et al. 2017

Methods in Molecular Biology

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“…The 5 0 and 3 0 ends of the ligation product contain a common sequence used as targets for a common set of primers for a further PCR amplification of the ligation product. This technique has been used successfully for the screening of multiple bioengineered products within the same reaction (Moreano et al, 2006;Ehlert et al, 2008). Detection of amplicons is carried out by capillary electrophoresis.…”

Section: Multiplexingmentioning

confidence: 99%

Detection of Food Allergens

Diaz‐Amigo

Pöpping

2009

Molecular Biological and Immunological Techniques and Applications for Food Chemists

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“…A very promising DNA detection method is the multiplex ligation depended probe amplification (MLPA) [8], which has already been applied in the area of medical diagnostics [9] or for the determination of allergens in food products [10] [11]. Moreano et al [12] and Ehlert et al [13] described the applicability of MLPA for the detection of gm events MONØØ81Ø-6 (MON810 corn) and MONØ4Ø32-6 (GTS 40-3-2 soybean), as well as for the detection of commonly used regulatory elements in gm crops like the P-35S promoter.…”

Section: Introductionmentioning

confidence: 99%

Development of a Multiplex Ligation Dependent Probe Amplification (MLPA) Module for Simultaneous Detection of Five Genetically Modified Rapeseed Events

Guertler¹,

Goerlich²,

Busch³

2014

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For the official surveillance of genetically modified crops, efficient and simple detection methods need to be on hand to implement the strict requirements regarding approval, labelling and traceability determined by the European Union. Therefore, a multiplex ligation dependent probe amplification (MLPA) module was developed for simultaneous detection of five genetically modified rapeseed events (MS8, RF3, GT73, Falcon GS40/90 and T45). Probes were designed and concentrations were adapted in order to obtain high sensitivity and specificity. This MLPA module was validated using certified reference materials and its applicability was tested analyzing routine honey, mustard and rapeseed samples. The limit of detection was determined by analyzing a dilution series (n = 16 for each concentration) of respective transgenic DNA. After optimization, the MLPA revealed limits of detection between 10 to 50 copies of the transgene DNA/assay. The method proved to be sensitive and highly reproducible. When analyzing routine samples, results obtained applying the MLPA module were similar compared to real-time PCR.

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Development of a modular system for detection of genetically modified organisms in food based on ligation-dependent probe amplification

Cited by 26 publications

References 34 publications

Recent Advances in the Detection of Allergens in Foods

Recent Advances in the Detection of Allergens in Foods

Detection of Food Allergens

Development of a Multiplex Ligation Dependent Probe Amplification (MLPA) Module for Simultaneous Detection of Five Genetically Modified Rapeseed Events

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