Development of a modified DNA chip for accurate human ABO genotyping

Abstract

In forensic investigations, ABO genotyping is often performed to obtain information for individual identiˆcation. This information is especially eŠective in the analysis of highly decomposed samples with which the serological method for ABO typing cannot be used due to ABH antigen deterioration. The authors previously reported on the development and improvement of a microarray-based method that can be applied for simultaneous ABO genotyping and human identiˆcation using two pairs of probes to detect major O or B allele-speciˆc single nucleotide polymorphisms (SNPs) in the ABO gene and a probe to determine the human-speciˆc