Development of a Model System to Compare Cell Separation Methods for the Isolation of Fetal Cells From Maternal Blood

Current and emerging techniques of fetal cell separation from maternal blood

“…Currently affinity based methods of cell separation such as FACS and MACS seem to dominate the research arena of NIPD with little research focusing on physical based methods of cell separation. Using these methods fewer than 20 fetal cells per 20 ml sample of maternal blood are obtained [68], as the extreme diversity of cells in blood raises serious challenges for their separation with high purity. There is also a non-negligible possibility that a large amount of fetal cells are being lost in the various discrete steps of separation and analysis.…”

Situación actual del diagnóstico prenatal no invasivo

“…Para la purificación de eritroblastos se han utilizado técnicas, como la separación celular mediante citometría de flujo (FACS) 10,12 , la separación celular activada magnéticamente (MACS) 23,34 , la micromanipulación de células individuales 35 , la separación por flujo de carga (CFS) 36 , el aislamiento con partículas inmunomagnéticas 37 , la suspensión líquida de micropartículas de hierro con separación magnética 38 o la separación en columnas con avidina 39 .…”

“…Otros anticuerpos utilizados son el anti-CD36, que reconoce el receptor de la trombospondina 25 , o los anti-CD45 y anti-CD32, que permiten el marcaje de las células contaminantes para su posterior eliminación 29,37 .…”

A magnetic colloid system for isolation of rare cells from blood for FISH analysis

“…More importantly, these cells may be isolated from maternal blood using monoclonal antibodies to target fetal cells (Bianchi et al, 1996;Durrant et al, 1996;Ganshirt et al, 1994;Hawes et al, 1994). Whereas the polymerase chain reaction (PCR) may be suitable for the identification of single gene disorders, chromosomal aneuploidies are detected by the use of FISH on interphase nuclei .…”