Development of a microtitre plate enzyme immunoassay for the determination of progesterone

Munro, Coralie; Stabenfeldt, G. H.

doi:10.1677/joe.0.1010041

Cited by 422 publications

(200 citation statements)

References 16 publications

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“…Blood samples were chilled on ice and centrifuged at 2,100 rpm for 15 min at 4 C. The plasma was aspirated without disturbing the buffy coat and frozen until needed. Plasma progesterone levels were determined by a rapid solid phase microtiter plate enzyme immunoassay (EIA), 24 using progesterone 3-0-carboxymethyloime-horseradish peroxidase as the label and antiserum raised in rabbits to a progesterone 11 alpha-hemisuccinyl bovine serum albumin immunogen. A competitive reaction was used with a reaction time of 2 hr.…”

Section: Methodsmentioning

confidence: 99%

Experimental Infection of Pregnant Cattle with Bluetongue Virus Serotype 11 between Postbreeding Days 21 and 48

et al. 1993

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Abstract. Four bluetongue virus (BTV)-seronegative heifers and 2 BTV-seropositive heifers were inoculated with the virulent strain UC-8 of BTV-11 between postbreeding days (PBD) 21 and 30. The heifers were observed for 10-18 days after inoculation for clinical signs, and pregnancy was monitored by ultrasound examination of the uterus and by plasma progesterone levels. Blood samples were collected daily after inoculation and processed for virus isolation and titration. Heifers were euthanized between PBD31 and PBD48, and tissues were collected for virologic and pathologic examination. All but 1 heifer inoculated on PBD21 remained pregnant after BTV inoculation, A cystic corpus luteum was found in the ovary of the nonpregnant heifer, but BTV was not isolated from the reproductive tract of this heifer. Three of the inoculated heifers that remained pregnant showed mild multifocal areas of perivascular lymphocytic infiltration in the ovary. BTV was reisolated from spleen and prescapular and peribronchial lymph nodes 10 days after inoculation from 3 of the 4 BTV-seronegative heifers. BTV was also reisolated from the uterus of 1 of the heifers that remained pregnant, but microscopic lesions were not found in this organ.

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Section: Methodsmentioning

confidence: 99%

Experimental Infection of Pregnant Cattle with Bluetongue Virus Serotype 11 between Postbreeding Days 21 and 48

et al. 1993

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“…Cortisol plasma levels were determined using an enzyme immunoassay (EIA). The technique was performed following the protocol developed by Munro and Stabenfeldt (1984) and modified by the Wisconsin Regional Primate Research CTR. Intra-and inter-assay coefficients of variation were 4.5 and 16.9% for low pool and 3.1 and 13.8% for high pool, respectively.…”

Section: Cortisolmentioning

confidence: 99%

Hormonal changes related to paternal and alloparental care in common marmosets (Callithrix jacchus)

Mota

Franci

Sousa

2006

Hormones and Behavior

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The physiological mechanisms of parental and alloparental care in cooperatively breeding nonhuman primate species such as the common marmoset (Callithrix jacchus) are poorly known. In this study, we examined prolactin and cortisol plasma levels of fathers and older offspring of both sexes, with and without previous experience in infant carrying, around parturition and during infant carrying. Blood samples were collected from fathers and older offspring and prolactin and cortisol were measured by RIA and EIA, respectively. Prolactin levels of both caretakers were not influenced by infant's birth, previous experience or proximity to parturition. However, prolactin levels increased in both caretakers while in physical contact with infants and also with the number of infants being carried in older offspring. These findings suggest that increased prolactin seems to be mainly due to physical effort rather than a physiological trigger of paternal and alloparental care in common marmosets. Cortisol levels were higher for experienced fathers shortly before parturition which could act to reinforce affiliative bonds between breeding males and females at this time or in the ability of males to detect the proximity of the parturition or both. D

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“…Cats that were before ovulation at insemination received the second administration immediately after insemination, while those after ovulation at insemination did not receive the second administration. To evaluate the success of induction of ovulation, the peripheral progesterone (P) level was measured 15 days after hCG administration by EIA [6] in cats that were pre-ovulatory at AI excluding pregnant animals, and when the P level was increased, induction of ovulation was judged successful.…”

Section: Animalsmentioning

confidence: 99%

Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

Tsutsui

Tanaka

Takagi

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The sperm count required were investigated to obtain a conception rate of 80% by unilateral intrauterine insemination (UIUI) of fresh semen in cats. The conception rates obtained by insemination before and after ovulation were also examined. Thirty-six female cats aged 1-7 years were used in the experiments, and the number of experimental cases was 44. Seven male cats aged 2-12 years from which semen could be collected by the artificial vagina method were used. In artificial insemination, 100 iu × 2 or 250 iu of hCG was administered on days 2-4 of estrus, and sperm were introduced into the uterine horn with a greater number of ovulations (or mature follicles) 15, 20 and 30 hr after hCG administration by laparotomy. The inseminated sperm counts were 2 × 10 6 (Exp. 1), 4 × 10 6 (Exp. 2), and 8 × 10 6 (Exp. 3). As a result, ovulation was induced in 42 of 44 cases (induction rate: 95.5%) regardless of the dosage of hCG. Conception was obtained by UIUI in two of 16 animals (conception rate: 12.5%) in the Exp. 1, five of 16 animals (31.3%) in Exp. 2, and eight of 10 animals (80.0%) in Exp. 3. Regarding the relationship between the ovulation state at insemination and conception, the conception rate obtained by insemination before ovulation was clearly higher than that obtained by insemination after ovulation (p<0.05). Regarding the number of kits compared to the number of ovulations on the inseminated side, the percentages of cases in which the number of kits exceeded the number of ovulations on the inseminated side were similar in all groups inseminated with a different number of sperm. It is therefore necessary to investigate conception rates obtained by bilateral insemination to increase the fertility rate. Based on the above findings, it was shown that the sperm count required for fertilization by UIUI is 8 × 10 6 . KEY WORDS: feline, fresh semen, intrauterine insemination.

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Development of a microtitre plate enzyme immunoassay for the determination of progesterone

Cited by 422 publications

References 16 publications

Experimental Infection of Pregnant Cattle with Bluetongue Virus Serotype 11 between Postbreeding Days 21 and 48

Experimental Infection of Pregnant Cattle with Bluetongue Virus Serotype 11 between Postbreeding Days 21 and 48

Hormonal changes related to paternal and alloparental care in common marmosets (Callithrix jacchus)

Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

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