Development of a Microfluidic Vibrational Cleaning System for Cleaning Microtissues

Thong, Kok Tung; Soon, Chin Fhong; Ismail, AB; Tee, Kian Sek

doi:10.1007/978-981-10-0266-3_31

Cited by 1 publication

(2 citation statements)

References 6 publications

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“…Whereas, vibration intensity represented the energy dissipation of the vibration signal that was measured directly from the microfluidic platform via an accelerometer. For cleaning the gel off the microtissues, 148 Hz and 0.89 Grms were applied and recommended to clean microtissues to yield high survival rate of cells [11]. Previous studies showed that the vibrational frequency and acceleration applied did not affect the viability of cells and it may even improve the differentiation and metabolism of cells as suggested in [12,13].…”

Section: The Vibration Frequencymentioning

confidence: 99%

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Hardware and circuit design of a vibrational cleaner

Soon

Thong

Tee

et al. 2016

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. Microtissue can be grown on soft substrates of hydrogel or liquid crystal gel. These gels are adherent to the microtissues and they may interfere fluorescence imaging as background noise due to their absorbance property. A microfluidic vibrational cleaner with polydimethylsiloxane (PDMS) microfluidic chip platform was proposed and developed to remove the residual gel of liquid crystal adhered to the microtissues. The microtissues were placed in a microfluidic chip attaching to a microfluidic vibrational platform. In the system design, two motorised vibrators vibrating attached to a microfluidic platform and generating vibration signals at 148 Hz and 0.89 Grms to clean the microtissues. The acceleration of the vibration increased gradually from 0 to 0.96 Grms when the duty cycle of PWM pulses increased from 50 -90%. It dropped slightly to 0.89 Grms at 100% duty cycle. Irrigation water valve was designed to control the fluid flow from water pump during cleaning process. Water pumps were included to flush the channels of the microfluidic device. The signals in controlling the pump, motor and valve were linearly proportional to the duty cycles of the pulse width modulation signals generated from a microcontroller.

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Section: The Vibration Frequencymentioning

confidence: 99%

“…A microfluidic vibrational cleaner was demonstrated to be effective in the removal of the liquid crystal gel residue found on the keratinospheroids [11]. However, the hardware and circuit system design have not been reported.…”

Section: Introductionmentioning

confidence: 99%