Development of a microfluidic system structured on a modified polydimethylsiloxane device for the selection of bovine epididymal spermatozoa

Fernandes, Beatriz Dantas; Santos, Fernanda Araújo dos; Bezerra, Luana Grasiele Pereira; Brito, Parmênedes Dias de; Macêdo, Luã Barbalho de; Silva, Alexandre Rodrigues; Bezerra, Marcelo Barbosa

doi:10.1016/j.reprotox.2022.03.005

Cited by 2 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results have driven men who suffer from azoospermia to be inclined to surgical sperm retrieval combined with IVM. However, there are few reports on its clinical outcome ( 14 , 15 ). In this study, we used different sperm sources to analyze the outcomes of ICSI using surgical sperm retrieval combined with IVM cycles, which may help patients make informed decisions.…”

Section: Discussionmentioning

confidence: 99%

Effects of different sperm sources on the clinical outcomes of in vitro oocyte maturation cycles combined with intracytoplasmic sperm injection

Chen²,

Tian³

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

ObjectivesTo evaluate the embryonic developments and clinical outcomes of different sperm sources with cycles of intracytoplasmic sperm injection (ICSI) and in vitro maturation (IVM).MethodsThis retrospective study was approved by the hospital ethics committee and conducted in the hospital in vitro fertilization (IVF) clinic. From January 2005 to December 2018, 239 infertile couples underwent IVM–ICSI cycles and were divided into three groups according to different sperm sources. Group 1 comprised patients with percutaneous epididymal sperm aspiration (PESA; n = 62, 62 cycles), group 2 comprised patients with testicular sperm aspiration (TESA; n = 51, 51 cycles), and group 3 comprised patients with ejaculated sperm (n = 126, 126 cycles). We calculated the following outcomes: 1) outcomes per IVM–ICSI cycle: fertilization rate, cleavage rate, and embryo quality; 2) outcomes per embryo transfer cycle: endometrial thickness, implantation rate, biochemical pregnancy rate, clinical pregnancy rate, and live birth rate.ResultsThere was no difference in basic characteristics among the three groups, such as the female partner’s age, basal follicle-stimulating hormone (FSH), basal luteinizing hormone (LH), and antral follicle count (p > 0.1). There were no statistically significant differences according to the IVM–ICSI cycle among the three groups in fertilization rate, cleavage rate, and rate of good-quality embryos (p > 0.05). The results were similar among cycles regarding the number of transfer embryos and endometrial thickness per embryo transfer cycle among the three groups (p > 0.05). There were also similar clinical outcomes per embryo transfer cycle among the three groups, such as the biochemical pregnancy rate, clinical pregnancy rate, and live birth rate (p > 0.05).ConclusionsDifferent sperm sources, percutaneous epididymal sperm aspiration, testicular sperm aspiration, and ejaculated sperm, do not affect the embryo and clinical outcomes after IVM–ICSI cycles.

show abstract

Section: Discussionmentioning

confidence: 99%