Development of a microfluidic paper-based analytical device for the determination of salivary aldehydes

Ramdzan, Adlin N.; Almeida, M. Inês G.S.; McCullough, Michael; Kolev, Spas D.

doi:10.1016/j.aca.2016.03.030

Cited by 40 publications

(11 citation statements)

References 35 publications

(51 reference statements)

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“…Paper-based analytical devices (PADs) as demonstrated by Whitesides's group 24 offer a promising solution for point-of-care diagnostics 25 - 27 . PADs can provide potentially relevant analysis for biomarker detection in many types of biological fluids, including tear fluids 28 , 29 , whole blood 30 - 32 , serum 33 , urine 34 and saliva 35 - 37 . To date, the development of PADs for saliva analysis have been reported for biomarkers such as nitrite 38 , thiocyanate 36 and aldehydes 37 using colorimetric assay and dengue specific antibodies using stacked flow immunoassay 35 .…”

Section: Introductionmentioning

confidence: 99%

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Noiphung¹,

Nguyen²,

Punyadeera³

et al. 2018

Theranostics

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Rationale: Saliva as a sample matrix is rapidly gaining interest for disease diagnosis and point-of-care assays because it is easy to collect (non-invasive) and contains many health-related biomarkers. However, saliva poses particular problems relative to more common urine and blood matrices, which includes low analyte concentrations, lack of understanding of biomolecule transportation and inherent viscosity variability in human samples. While several studies have sought to improve assay sensitivity, few have addressed sample viscosity specifically. The goal of this study is to minimize the effect of sample viscosity on paper-based analytical devices (PADs) for the measurement of pH and nitrite in human saliva.Methods: PADs were used to measure salivary pH from 5.0 to 10.0 with a universal indicator consisting of chlorophenol red, phenol red and phenolphthalein. Nitrite determination was performed using the Griess reaction. Artificial saliva with viscosity values between 1.54 and 5.10 mPa∙s was tested on the proposed PAD. To ensure the proposed PADs can be tailored for use in-field analysis, the devices were shipped to Australia and tested with human specimens.Results: Initial experiments showed that viscosity had a significant impact on the calibration curve for nitrite; however, a more consistent curve could be generated when buffer was added after the sample, irrespective of sample viscosity. The linear range for nitrite detection was 0.1 to 2.4 mg/dL using the improved method. The nitrite measurement in artificial saliva also showed a good correlation with the standard spectrophotometry method (p=0.8484, paired sample t-test, n=20). Measured pH values from samples with varying viscosities correlated well with the results from our pH meter.Conclusions: The inherent variation of salivary viscosity that impacts nitrite and pH results can be addressed using a simple washing step on the PAD without the need for complex procedures.

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Section: Introductionmentioning

confidence: 99%

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Noiphung¹,

Nguyen²,

Punyadeera³

et al. 2018

Theranostics

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“…Using the clinical result as a relative concentration compensates for the urine volume [47], and also avoids adulteration by dilution [42]. Therefore, in addition to the number of mPADs described in the literature for glucose [22], protein [22], nitrite [23], aldehydes [24], beta-hydroxybutyrate [25], uric acid [15], and cholesterol [26], with the proposed device, the methods of clinical analysis are increased, making possible the evaluation of several diseases in a single mPAD and compensating the urine dilution.…”

Section: Discussionmentioning

confidence: 99%

“…This inexpensive diagnostic platform may be employed in remote villages or other areas where it is difficult to access laboratory infrastructure and trained personnel, due to its simplicity and minimal production cost. For this reason, techniques have been developed for the detection of several species relevant for disease prevention and diagnosis, including glucose [22], protein [22], nitrite [23], aldehydes [24], beta-hydroxybutyrate [25], uric acid [15], and cholesterol [26].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Rossini

Milani

Carrilho

et al. 2018

Analytica Chimica Acta

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In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe to Fe by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L and 50-600 mg L for CRN and 16.5 mg L and 50-500 mg L for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).

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“…It defines the channels through which the liquid flows in the paper fibers by means of dipping wax, then separates the blood plasma and blood cells through the pores of the paper fibers, and finally determines the content of serum protein by means of coloration. Paper-based microfluidic chips have been widely used in various medical and life science detection research and applications, due to the low cost of materials and processing, such as saliva acetaldehyde detection [45], heavy metal detection [46], blood sugar detection [47], lactic acid detection [48], etc.…”

Section: Paper Materialsmentioning

confidence: 99%

Application of Microfluidic Chip Technology in Food Safety Sensing

Gao

Yan

et al. 2020

Sensors

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Food safety analysis is an important procedure to control food contamination and supervision. It is urgently needed to construct effective methods for on-site, fast, accurate and popular food safety sensing. Among them, microfluidic chip technology exhibits distinguish advantages in detection, including less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability. In this review, we introduce the classification, material, processing and application of the microfluidic chip in food safety sensing, in order to provide a good guide for food safety monitoring.

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Development of a microfluidic paper-based analytical device for the determination of salivary aldehydes

Cited by 40 publications

References 35 publications

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Development of Paper-Based Analytical Devices for Minimizing the Viscosity Effect in Human Saliva

Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Application of Microfluidic Chip Technology in Food Safety Sensing

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