Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos

Karcz, Adriana; Soom, Ann Van; Smits, Katrien; Vlierberghe, Sandra Van; Verplancke, Rik; Pascottini, Osvaldo Bogado; Abbeel, E. Van den; Vanfleteren, Jan

doi:10.3390/bios13040419

Cited by 6 publications

(7 citation statements)

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“…These methods often involve micro and nanofabrication techniques (M.J. Lopez-Martinez & E.M. Campo, 2011). One innovative approach, which utilizes electrowetting-on-dielectric technology, is the creation of microfluidic chips that are capable of manipulating bovine embryos in vitro (Karcz et al, 2023). By integrating nanotechnology with various other fields, researchers have developed unique nanomaterials that can revolutionize diagnostic and therapeutic applications (Barhoum et al, 2022).…”

Section: Nanomaterials In Embryo Manipulationsmentioning

confidence: 99%

“…Nanomaterials could be the answer to bettering the quality of oocytes, which is essential for IVF success.To achieve this, on-chip manipulation of culture medium microdroplets and electrical stimulation of embryos and gametes have displayed encouraging results in a study conducted by Karcz et al (2023). Additionally, Morimoto et al (2023) found that mitochondrial transfer to human oocytes leads to acceptable clinical outcomes, enhancing embryo quality.…”

Section: Applications Of Nanomaterials In In Vitro Fertilization (Ivf)mentioning

confidence: 99%

“…They have been utilized in delivering target molecules within the intra-gamete and embryo, thus improving assisted reproductive techniques (Lucas et al, 2019). As evidenced in animal studies, nanomaterials can aid in sperm selection, provide antioxidants and hormones to preantral follicles, and even manipulate bovine embryos in vitro ( Karcz et al, 2023;Silva et al, 2021). Remarkably, Eto et al in 2021 were able to produce genetically modified animals using fully automated nuclear injection (Eto et al, 2021).…”

Section: Current Research On Nanomaterials In Embryo Manipulationsmentioning

confidence: 99%

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Nanotechnology in the Treatment of Infertility

Narlıçay,

Kıvrak

2023

Nanotechnology in Reproduction

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With the increasing reproductive issues in all living beings worldwide, numerous problems related to fertility have emerged. Concurrently, the rising global population rates have led to a situation where the available food resources cannot meet the demand. Environmental factors, genetics, age, nutrition, stress, and global changes are some of the reasons that lead to infertility problems. Efforts are still underway to find methods in vivo or in vitro studies to eliminate reproductive problems by preventing structural abnormalities or changes in germ cells. In recent years, some nanoparticles have been utilized to address infertility issues caused by hormonal imbalances, metabolic disorders, or abnormalities in gamete cells, aiming to perform manipulations that could positively impact fertilization. Nanoparticles, used at non-toxic levels, have shown positive responses in fertility. Particularly in spermatological studies, nanoantioxidants have been observed to reduce reactive oxygen species, consequently preventing oxidative stress. Additionally, they are reported to benefit motility and sperm viability, while preserving DNA and gene expression. This section provides important insights into the potential of nanobiotechnology in addressing infertility issues in the years to come.

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Section: Nanomaterials In Embryo Manipulationsmentioning

confidence: 99%

Section: Applications Of Nanomaterials In In Vitro Fertilization (Ivf)mentioning

confidence: 99%

Section: Current Research On Nanomaterials In Embryo Manipulationsmentioning

confidence: 99%

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Nanotechnology in the Treatment of Infertility

Narlıçay,

Kıvrak

2023

Nanotechnology in Reproduction

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“…В животноводстве для получения и сохранения высококачественных женских гамет широко применяют вспомогательные репродуктивные технологии (ВРТ). Цикл ВРТ включает извлечение ооцитов, созревание in vitro (IVM), экстракорпоральное оплодотворение (ЭКО) или интрацитоплазматическую инъекцию сперматозоидов (ИКСИ), трансплантацию эмбрионов (ТЭ), а также криоконсервацию гамет или эмбрионов [1]. Криоконсервация -уникальный метод для сохранения половых клеток различных видов млекопитающих, ценных пород сельскохозяйственных животных в условиях стремительного снижения биоразнообразия.…”

Section: Introductionunclassified

Modernization of the cryoprotective medium compound for intraovarian vitrification of female gametes of <em>Sus scrofa domesticus</em>

Kuzmina,

Starikova

2024

jour

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Aim. Comprehensive analysis of the morphofunctional state of somatic (cumulus) and germ cells (oocytes) of Sus scrofa domesticus subjected to intraovarian vitrification using silicon dimethylglycerolate (SDMG) are presented.Materials and methods. Fragments of porcine ovaries (FsPO) 15×20 mm in size were gradually kept in cryoprotective agents (CPA) prepared in phosphate-buffered saline (PBS) with 20% fetal bovine serum (FBS): 25 min. in CPA-1 [7.5% EG (ethylene glycol) with 7.5% DMSO (dimethyl sulfoxide)] and 15 min. in CPA-2 (15% EG with 15% DMSO and 0.5 M sucrose). The composition of the CPA-2 in experimental groups was modified by addition of SDMG (at concentrations of 2%, 6%, or 10%). FsPO were stored in liquid nitrogen. FsPO were devitrified by exposure 1 minute in solution 1 (80% PBS, 20% FBS, 0.5 mol/l sucrose) and 5 minutes in solution 2 (80% PBS, 0.25 mol/l sucrose). The following indicators of cryoresistance of devitrified cumulus-oocyte complexes (COCs) were analyzed: degree of cumulus cells expansion; oocyte morphology and the functional status of lipidome in female gametes (fluorescence intensity of Nile red /lipid droplets complex - FILDs).Results. The addition of SDMG into cryoprotective media reduced the level of denuded oocytes after vitrification. The level of gamete with different degree of cumulus cells expansion (low, medium, high) in the experimental group with 10% SDMG tended to indicators in the group of native cells. The level of native oocytes with the signs of morphological degeneration (7.7%) had no significant differences with the level of intraovarian vitrified gametes with 10% SDMG (11%). The proportion of native oocytes with low FILDs (38.9%) exceeded the level of oocytes with the above indicator in vitrified oocytes of the control (16.5%) group and in experimental groups of cells with the addition of 6% SDMG (4.8%) and 10% SDMG (11.8%, P<0.001).Conclusion. In general, comprehensive monitoring of indicators cryoresistance of COCs in Sus scrofa domesticus subjected to intraovarian vitrification revealed the cryoprotective properties of SDMG. The effects were dose-dependent and were expressed in the stabilization of oocyte-cumulus communication, a decrease in the level of oocytes with the signs of morphological degeneration, and features of the lipidome functioning in intraovarian vitrified female gametes using SDMG at various concentrations.

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“…Microfluidics is a rapidly growing field that involves the manipulation of small volumes of fluids in microscale channels, offering precise control over the cellular microenvironment ( Whitesides, 2006 ), superior nutrient and waste exchange ( Berthier et al, 2012 ), and the potential for high-throughput screening ( Sackmann et al, 2014 ). This technology has been increasingly applied to various aspects of IVEP, including oocyte and sperm handling ( Beckham et al, 2018 ; Wagenaar et al, 2015 ; El-Sherry et al, 2014 ; Scherr et al, 2015 ), in vitro fertilization (IVF) ( Clark et al, 2005 ; Huang et al, 2018 ; Suh et al, 2006 ), and embryo culture and assessment ( Esteves et al, 2013 ; Huang et al, 2015 ; Karcz et al, 2023 ; Kieslinger et al, 2015 ; Kim et al, 2009 ). 3D culture systems, on the other hand, provide a more physiologically relevant environment for cell growth and differentiation compared to traditional 2D culture systems ( Baker and Chen, 2012 ).…”

Section: Introductionmentioning

confidence: 99%