Development of a method to produce standardised and storable inocula for biomethane potential tests – Preliminary steps

Heerenklage, J.; Rechtenbach, D.; Atamaniuk, Iryna; Alassali, Ayah; Raga, Roberto; Koch, Konrad; Kuchta, Kerstin

doi:10.1016/j.renene.2019.05.037

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…The development of a method to produce standardized inoculum for a range of substrate in anaerobic digestion batch tests are estimated to increase reproductivity [80]. As of 2017, the Hamburg University of Technology (TUHH) are researching long term preservation methods for anaerobic inocula.…”

Section: Standardized Inoculummentioning

confidence: 99%

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Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Filer

Ding

Chang

2019

Water

265

144

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Biochemical methane potential (BMP) tests are widely used for characterizing a substrate’s influence on the anaerobic digestion process. As of 2018, there continues to be a lack of standardization of units and techniques, which impacts the comparability and validity of BMP results. However, BMP methods continue to evolve, and key aspects are studied to further eliminate systematic errors. This paper aims to update these key aspects with the latest research progress both to introduce the importance of each variable to those new to BMP measurements and to show the complexity required to design an accurate BMP test.

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Section: Standardized Inoculummentioning

confidence: 99%

“…But, the authors reported a 7-10-day lag phase of methane production possibly due to the damage of microorganism by the preservation method. Further investigations on the optimize preservation process are required [80].…”

Section: Standardized Inoculummentioning

confidence: 99%

Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Filer

Ding

Chang

2019

Water

265

144

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“…In Europe, the biogas is mainly produced by the anaerobic digestion of agricultural residuals, manure, and energy crops. Additionally, the sludge of wastewater treatment plants, organic fraction of the municipal solid waste, or solid waste buried in landfills are possible feedstock sources [10,13,15]. The anaerobic digestion is an efficient method to treat the organic fraction of the municipal solid waste for energy production while mitigating the greenhouse emissions [16][17][18].…”

Section: Substratesmentioning

confidence: 99%

Operational Parameters of Biogas Plants: A Review and Evaluation Study

et al. 2020

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The biogas production technology has improved over the last years for the aim of reducing the costs of the process, increasing the biogas yields, and minimizing the greenhouse gas emissions. To obtain a stable and efficient biogas production, there are several design considerations and operational parameters to be taken into account. Besides, adapting the process to unanticipated conditions can be achieved by adequate monitoring of various operational parameters. This paper reviews the research that has been conducted over the last years. This review paper summarizes the developments in biogas design and operation, while highlighting the main factors that affect the efficiency of the anaerobic digestion process. The study’s outcomes revealed that the optimum operational values of the main parameters may vary from one biogas plant to another. Additionally, the negative conditions that should be avoided while operating a biogas plant were identified.

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“…Both calculated results based on measured biomass yields and measured BMP values show significant variability in cellulose BMP [14][15][16]. For instance, Heerenklage et al [17] found that the amount of methane recovered from CEL was significantly lower for a lyophilized inoculum compared to a fresh one. They concluded that the microbial biomass yield was higher for the lyophilized inoculum due to the damaging conservation step.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Common Supermarket Products as Positive Controls in Biochemical Methane Potential (BMP) Tests

Koch

Hafner²,

Astals

et al. 2020

Water

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Biochemical methane potential (BMP) tests are commonly applied to evaluate the recoverable amount of methane from a substrate. Standardized protocols require inclusion of a positive control with a known BMP to check the experimental setup and execution, as well as the performance of the inoculum. Only if the BMP of the positive control is within the expected range is the entire test validated. Besides ignorance of this requirement, limited availability of the standard positive control microcrystalline cellulose might be the main reason for neglecting a positive control. To address this limitation, eight widely available grocery store products have been tested as alternative positive controls (APC) to demonstrate their suitability. Among them, Tic Tacs and gummi bears were very promising, although they are dominated by easily degradable sugars and so do not test for hydrolytic performance. Coffee filters exhibited a similar performance to microcrystalline cellulose, while whole milk might be chosen when a more balanced carbohydrate:protein:lipid ratio is important. Overall, the approach of predicting the BMP of a substrate based on the nutritional composition provided on the product packaging worked surprisingly well: BMP of the eight tested products was 81–91% of theoretical maximum BMP based on nutritional information and generic chemical formulas for carbohydrates, proteins, and lipids.

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Development of a method to produce standardised and storable inocula for biomethane potential tests – Preliminary steps

Cited by 13 publications

References 18 publications

Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research

Operational Parameters of Biogas Plants: A Review and Evaluation Study

Evaluation of Common Supermarket Products as Positive Controls in Biochemical Methane Potential (BMP) Tests

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