Development of a Method for Obtaining Bromelain Associates with Chitosan Micro- and Nanoparticles

Red’ko, Yu. A.; Olshannikova, Svetlana; Holyavka, M. G.; Lavlinskaya, Maria S.; Сорокин, А. В.; Artyukhov, V. G.

doi:10.1007/s11094-022-02737-5

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Our previously studies show that natural polymers, such as chitosan [2,14] and its derivatives [21][22][23][24] or nanoparticles based on it [25,26] are promising carriers for cysteine protease immobilization. This process can lead to enhance of proteolytic activity and make it possible to use immobilized enzymes as antibacterial and antibiofilm agents as well as in wound healing [2,27].…”

Section: Introductionmentioning

confidence: 99%

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Сорокин

Goncharova

Lavlinskaya

et al. 2023

IJMS

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This study investigates the features of interactions between cysteine proteases (bromelain, ficin, and papain) and a graft copolymer of carboxymethyl cellulose sodium salt with N-vinylimidazole. The objective is to understand the influence of this interactions on the proteolytic activity and stability of the enzymes. The enzymes were immobilized through complexation with the carrier. The interaction mechanism was examined using Fourier-transform infrared spectroscopy and flexible molecular docking simulations. The findings reveal that the enzymes interact with the functional groups of the carrier via amino acid residues, resulting in the formation of secondary structure elements and enzyme’s active sites. These interactions induce modulation of active site of the enzymes, leading to an enhancement in their proteolytic activity. Furthermore, the immobilized enzymes demonstrate superior stability compared to their native counterparts. Notably, during a 21-day incubation period, no protein release from the conjugates was observed. These results suggest that the complexation of the enzymes with the graft copolymer has the potential to improve their performance as biocatalysts, with applications in various fields such as biomedicine, pharmaceutics, and biotechnology.

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Section: Introductionmentioning

confidence: 99%

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Сорокин

Goncharova

Lavlinskaya

et al. 2023

IJMS

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Novel biocatalysts based on enzymes in complexes with nano- and micromaterials

Holyavka,

Goncharova,

Redko

et al. 2023

Biophys Rev

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Recent Progress in Chitosan-Containing Composite Materials for Sustainable Approaches to Adsorption and Catalysis

et al. 2023

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In recent years, composite materials including organic–inorganic systems have drawn special attention due to their enhanced properties such as adsorbents and heterogeneous catalysts. At the same time, large-scale production of environmentally benign functionalized biopolymers, such as chitosan (CS), allows for constantly developing new materials, since CS reveals remarkable properties as a stabilizing agent for metal-containing compounds and enzymes and as an adsorbent of organic molecules. This review is focused on CS-based materials and on the composite systems including CS–oxide and CS–metal composites in particular for application as adsorbents and supports for catalytically active metal nanoparticles and enzymes.

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Development of a Method for Obtaining Bromelain Associates with Chitosan Micro- and Nanoparticles

Cited by 3 publications

References 11 publications

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Complexation of Bromelain, Ficin, and Papain with the Graft Copolymer of Carboxymethyl Cellulose Sodium Salt and N-Vinylimidazole Enhances Enzyme Proteolytic Activity

Novel biocatalysts based on enzymes in complexes with nano- and micromaterials

Recent Progress in Chitosan-Containing Composite Materials for Sustainable Approaches to Adsorption and Catalysis

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