Development of a method for optical monitoring of creatinine in the spent dialysate

Abstract

The aim of the study was to develop a method suitable for the estimation of the amount of creatinine, removed during dialysis through UV-absorbance. Sixteen uremic patients, seven females and nine males, on chronic thrice-weekly hemodialysis were included in the study. Doublebeam spectrophotometer was used for the determination of UV-absorbance in the collected spent dialysate samples. Due to differences in independent variables, two multi-wavelength models (m1 and m2) were developed using stepwise regression, utilizing creatinine values from the laboratory as the dependent parameter. The coefficient of determination, R 2 was 0.8690 for the first and 0.9103 for the second model. The systematic error, estimated as BIAS, was zero for both models compared to the creatinine values from the laboratory. The standard errors were 10.06 µmol/l and 15.24 µmol/l for m1 and m2, respectively. The average reduction ratio (RR) from creatinine blood values was 59.8 ± 5.4% (N = 50), average RR from m1 was 63.7 ± 7.3% (N = 50) and average RR from m2 was 64.8 ± 6.4% (N = 48). In summary, the amount of creatinine removed as well as the reduction ratio of creatinine during dialysis can be estimated with UV-absorbance technique.

Determination of creatinine level in patient blood samples by Fourier NIR spectroscopy and multivariate analysis in comparison with biochemical assay

“…9,10 Based on NIR absorption spectrum of urine samples and multivariate regression model Cr prediction accuracy of 9 mg/dL was reported by Shaw et al 6 The estimation the amount of Cr in the spent dialysate using UV-absorbance approach has been reported which does not need blood samples and disposables of chemicals and is fast. 12 Rapid determination of Cr in human urine-based°uorescence detection for evaluating renal function was documented from the study of Wang et al 13 Surface enhanced Raman scattering (SERS) has been also proposed by few groups for the detection of Cr. 11,23 Nevertheless, as far as we are aware, our observed clinical Cr blood concentration near patient's bedside is lower than those in the reported literatures which primarily measure Cr in urine or serum samples.…”

Solubility of Thiotriazinone in Binary Solvent Mixtures of Water + Methanol and Water + Ethanol from (283 to 330) K

“…Thus, it is a simplification to keep only the statistically significantly variables in the model. In this work, the stepwise regression method was taken to study the significance of each independent variable for the regression equation . The significant variables were added, while the insignificant variables were removed.…”

“…In this work, the stepwise regression method was taken to study the significance of each independent variable for the regression equation. 11 The significant variables were added, while the insignificant variables were removed. The conditions of adding independent variables are: the independent variable is the most prominent among all the independent variables by the inspection of partial regression square.…”

Waves, oscillations and optics in biomedical engineering: research in the Department of Biomedical Engineering 1994–2014

“…The biofluid optics research in the DBME is based on a novel concept of the multicomponent dialysis monitoring, using optical techniques. The multicomponent monitor incorporates online removal estimation of all the three uremic toxins' groups: small molecular weight, middle molecular weight, and protein bound large molecular weight toxins [50][51][52][53][54][55][56][57][58][59][60].…”