Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves

Courant, Frédérique; Pinel, Gaud; Bichon, Emmanuelle; Monteau, Fabrice; Antignac, Jean‐Philippe; Bizec, Bruno Le

doi:10.1039/b901813a

Cited by 110 publications

(75 citation statements)

References 38 publications

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“…Metabolomic LC-HRMS profiles were acquired from 15 ml of each filtered serum sample using an Agilent 1200 HPLC system equipped with a 150!2.1 mm Uptisphere HDO-C 18 column with 3 mm particle size (Interchim, Montluçon, France) coupled to a high-resolution LTQOrbitrap hybrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) fitted with an electrospray source operated in the positive ion mode. The detailed conditions applied both for the HPLC separation and mass spectrometric signal acquisition have been described previously (Courant et al 2009, Alexandre-Gouabau et al 2011. Quality control standards and samples were randomly included five times into the sequence of injection.…”

Section: Laboratory Analysismentioning

confidence: 99%

Effect of pre- and postnatal growth and post-weaning activity on glucose metabolism in the offspring

Dellschaft¹,

Alexandre-Gouabau²,

Gardner³

et al. 2014

Journal of Endocrinology

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Maternal caloric restriction during late gestation reduces birth weight, but whether long-term adverse metabolic outcomes of intra-uterine growth retardation (IUGR) are dependent on either accelerated postnatal growth or exposure to an obesogenic environment after weaning is not established. We induced IUGR in twin-pregnant sheep using a 40% maternal caloric restriction commencing from 110 days of gestation until term (w147 days), compared with mothers fed to 100% of requirements. Offspring were reared either as singletons to accelerate postnatal growth or as twins to achieve standard growth. To promote an adverse phenotype in young adulthood, after weaning, offspring were reared under a low-activity obesogenic environment with the exception of a subgroup of IUGR offspring, reared as twins, maintained in a standard activity environment. We assessed glucose tolerance together with leptin and cortisol responses to feeding in young adulthood when the hypothalamus was sampled for assessment of genes regulating appetite control, energy and endocrine sensitivity. Caloric restriction reduced maternal plasma glucose, raised non-esterified fatty acids, and changed the metabolomic profile, but had no effect on insulin, leptin, or cortisol. IUGR offspring whose postnatal growth was enhanced and were obese showed insulin and leptin resistance plus raised cortisol. This was accompanied by increased hypothalamic gene expression for energy and glucocorticoid sensitivity. These long-term adaptations were reduced but not normalized in IUGR offspring whose postnatal growth was not accelerated and remained lean in a standard post-weaning environment. IUGR results in an adverse metabolic phenotype, especially when postnatal growth is enhanced and offspring progress to juvenile-onset obesity.

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Section: Laboratory Analysismentioning

confidence: 99%

Effect of pre- and postnatal growth and post-weaning activity on glucose metabolism in the offspring

Dellschaft¹,

Alexandre-Gouabau²,

Gardner³

et al. 2014

Journal of Endocrinology

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“…As no definitive and unique standard operation procedures currently exist, the comparative generation and analysis of data obtained from identical samples but acquired through different analytical conditions should help the interpretation of the information generated. Such developments relating to analysis and comparisons have recently been conducted in several laboratories, [12][13][14][15][16][17][18] leading to the formulation of in-house strategies for each of the steps of interest.…”

Section: Metabolomics Workflow To Highlight Biomarkers Of Anabolic Prmentioning

confidence: 99%

“…Typical reported protocols for the investigation of breeding animals' metabolome to highlight anabolic practices have involved at least ten animals divided into control and treated groups. [14][15][16][17][18] Regarding biological matrices of interest to be collected during the animal experiment, urine, serum or plasma are easy to collect as often as necessary and can be directly collected at the farm for doping control purposes. In addition, plasma and urine provide very complementary information about the state of an organism.…”

Section: Animal Experiments and Biological Samples Of Interestmentioning

confidence: 99%

“…The relevance and efficiency of this protocol has already been demonstrated through various applications dedicated to the screening of anabolic practices in cattle. [12,[14][15][16][17][18] In particular, the specificity related to the different biological matrices that may be used to perform a metabolomics study will be discussed.…”

Section: Introductionmentioning

confidence: 99%

“…[12] In particular, metabolomics has recently drawn the attention of the scientific community in the detection of anabolic practices and several studies have indeed demonstrated the efficiency of such mass-spectrometric-based fingerprinting to discriminate animals that have been subjected to anabolic treatment from control animals. Several approaches have shown potential to discriminate through analysis of urine samples of animals treated with a range of anabolic formulations: DHEA and pregnenolone in bovines, [13] clenbuterol in calves, [14] recombinant equine growth hormone in horses, [15][16][17] and nandrolone and estradiol in calves. [18] Metabolomics is an emerging field of 'omics' research that focuses, in an untargeted mode, on large-scale and high-throughput measurement of small molecules (so-called metabolites) in biological matrices.…”

Section: Introductionmentioning

confidence: 99%

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Metabolomics in food analysis: application to the control of forbidden substances

Dervilly

Courant

Chéreau

et al. 2012

Drug Testing and Analysis

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Metabolomics is a science of interest in food analysis to describe and predict properties of food products and processes. It includes the development of analytical methods with the ultimate goal being the identification of so-called 'quality markers', (i.e. sets of metabolites that correlate with, for example, quality, safety, taste, or fragrance of foodstuffs). In turn, these metabolites are influenced by factors as genetic differences of the raw food ingredients (such as animal breed or crop species differences), growth conditions (such as climate, irrigation strategy, or feeding) or production conditions (such as temperature, acidity, or pressure). In cases where the routine-based measurement of a food property faces some limitations such as the lack of knowledge regarding the target compounds to monitor, monitoring based on a limited set of crucial biomarkers is a good alternative, which is of great interest for food safety purposes regarding growth promoting practices. Such an approach may be more efficient than using a classic approach based on a limited set of known metabolites of anabolic compounds. In this context, screening strategies allowing detection of the physiological response resulting from anabolic compound administration are promising approaches to detect their misuse. The global metabolomics workflow implemented for such studies is presented and illustrated through various examples of biological matrices profiling (tissue, blood, urine) and for different classes of anabolic compounds (steroids, b-agonists and somatotropin).

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Capabilities and Limitations of High-Resolution Mass Spectrometry (HRMS): Time-of-flight and Orbitrap™

Kaufmann¹,

Teale²

2016

Chemical Analysis of Non&;#x02010;antimicrobial Veterinary Drug Residues in Food

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Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves

Cited by 110 publications

References 38 publications

Effect of pre- and postnatal growth and post-weaning activity on glucose metabolism in the offspring

Effect of pre- and postnatal growth and post-weaning activity on glucose metabolism in the offspring

Metabolomics in food analysis: application to the control of forbidden substances

Capabilities and Limitations of High-Resolution Mass Spectrometry (HRMS): Time-of-flight and Orbitrap™

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