2007
DOI: 10.1118/1.2795665
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Development of a mechanical testing assay for fibrotic murine liver

Abstract: In this article, a novel protocol for mechanical testing, combined with finite element modeling, is presented that allows the determination of the elastic modulus of normal and fibrotic murine livers and is compared to an independent mechanical testing method. The novel protocol employs suspending a portion of murine liver tissue in a cylindrical polyacrylamide gel, imaging with a microCT, conducting mechanical testing, and concluding with a mechanical property determination via a finite element method analysi… Show more

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Cited by 20 publications
(15 citation statements)
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“…In contrast, liver tissue exhibited the most compliant response among all samples. The comparative compliance of liver tissue as compared to heart tissue at these elevated deformation rates is consistent with quasistatic mechanical comparisons of elastic moduli, E, between two tissue types, estimated via macroscale uniaxial compression, uniaxial tension, and indentation experiments [6,7,36,37].…”
Section: Philosophical Magazine 1349supporting
confidence: 72%
See 1 more Smart Citation
“…In contrast, liver tissue exhibited the most compliant response among all samples. The comparative compliance of liver tissue as compared to heart tissue at these elevated deformation rates is consistent with quasistatic mechanical comparisons of elastic moduli, E, between two tissue types, estimated via macroscale uniaxial compression, uniaxial tension, and indentation experiments [6,7,36,37].…”
Section: Philosophical Magazine 1349supporting
confidence: 72%
“…A growing number of reports have quantified mechanical properties of soft tissues via a variety of quasistatic methods including macroscale tension, unconfined compression and microscale indentation experiments [4][5][6][7][8][9][10][11]. Further, dynamic mechanical analysis of soft tissues have been explored via methods such as oscillatory loading [12][13][14][15] and indentation recovery [16].…”
Section: Introductionmentioning
confidence: 99%
“…The assay is called the model-gel-tissue (MGT) assay, and is used here to demonstrate the link between tissue mechanical properties and collagen content for mammary tumors. Preliminary work utilizing the assay within a liver fibrosis murine model has been reported ( Barnes et al 2007). In this work, the utility of the assay to evaluate various tissue types is demonstrated, as a shift is made from fibrotic liver to mammary tumor tissue.…”
Section: Introductionmentioning
confidence: 98%
“…Assessment of tissue stiffness often plays an integral role in research and clinical diagnostics since it has been identified as an indicator of a variety of pathological conditions, including hepatic fibrosis, arterial disease, myocardial infarction, breast cancer, prostatic cancer, thyroid disease, and skin cancer, among others (Anderson 1980;Covell and Ross 1973;de Ledinghen et al 2006;Diamond and Forrester 1972;Gaasch et al 1976;Giannattasio and Mancia 2002;Herrington et al 2004;Krouskop et al 1998;Levy et al 2001;Nagasaki et al 2006;Phipps et al 2005;Tilleman et al 2004;vanPopele et al 2001;Yeh et al 2002;Ziol et al 2005). In addition, recently developed medical imaging modalities are attempting to render non-invasive measurements of these properties for disease screening (commonly cancer screening) and therapeutic applications (Bilgen et al 2003;Fahey et al 2008;Garra et al 1997;Muthupillai et al 1995;Ophir et al 1991;Sandrin et al 2003;Sinkus et al 2008;Souchon et al 2003;Venkatesh et al 2008).…”
Section: Introductionmentioning
confidence: 99%
“…The concept of an inverse FE analysis method to determine the mechanical parameters to monitor the progression of fibrogenic diseases has been demonstrated. These techniques are more widely referred to as elastography (Ophir et al, 1991; Greenleaf et al, 2003; Washington and Miga, 2004; Miga et al, 2005; Barnes et al, 2007; Samani and Plewes, 2007; Ou et al, 2008). Within this work, the approach is used to evaluate mechanical properties as a biomarker in the assessment of fracture healing progression.…”
Section: Introductionmentioning
confidence: 99%