Development of a mechanical sexing system to improve the efficacy of an area‐wide sterile insect release programme to control American serpentine leafminer (Diptera: Agromyzidae) in Canadian ornamental greenhouses

Sultan, Maryam; Buitenhuis, Rosemarije; Murphy, Graeme; Scott‐Dupree, Cynthia

doi:10.1002/ps.4494

Cited by 3 publications

(3 citation statements)

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“…The lack of a standardized and efficient method for isolating males is an obstacle for the IIT ( Lutrat et al 2019 , Baton et al 2021 ). Male selection in L. trifolii using a 1.54 × 0.76-mm pore sieve is based on pupal sexual dimorphism, although the effectiveness of this method is less than 100% ( Sultan et al 2017 ). Field contamination with Wolbachia -infected female L. trifolii can lead to the unintended replacement of a Wolbachia -infected population due to their compatibility with released males of the same strain as well as with uninfected wild males in the field.…”

Section: Discussionmentioning

confidence: 99%

Effect of Quorum Sensing Inducers and Inhibitors on Cytoplasmic Incompatibility Induced byWolbachia(Rickettsiales: Anaplasmataceae) in American Serpentine Leafminer (Diptera: Agromyzidae): Potential Tool for the Incompatible Insect Technique

Hidayanti

Gazali

Tagami

2022

Journal of Insect Science

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Agricultural crops around the world are attacked by approximately 3,000–10,000 species of pest insect. There is increasing interest in resolving this problem using environmentally friendly approaches. Wolbachia (Hertig), an insect endosymbiont, can modulate host reproduction and offspring sex through cytoplasmic incompatibility (CI). The incompatible insect technique (IIT) based on CI-Wolbachia is a promising biological control method. Previous studies have reported an association between CI and Wolbachia density, which may involve a quorum sensing (QS) mechanism. In this study, we investigated the effect of manipulating QS in Wolbachia using several chemicals including 3O-C12-HSL; C2HSL; spermidine (QS inducers), 4-phenylbutanoyl; and 4-NPO (QS inhibitors) on American serpentine leafminer (Liriomyza trifolii [Burgess]), an agricultural pest. The results showed that inducing QS with 3O-C12-HSL decreased the proportion of hatched eggs and increased Wolbachia density, whereas QS inhibition with 4-phenylbutanoyl had the opposite effects. Thus, manipulating QS in Wolbachia can alter cell density and the proportion of hatched eggs in the host L. trifolii, thereby reducing the number of insect progeny. These findings provide evidence supporting the potential efficacy of the IIT based on CI-Wolbachia for the environmentally friendly control of insect pest populations.

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Section: Discussionmentioning

confidence: 99%

Effect of Quorum Sensing Inducers and Inhibitors on Cytoplasmic Incompatibility Induced byWolbachia(Rickettsiales: Anaplasmataceae) in American Serpentine Leafminer (Diptera: Agromyzidae): Potential Tool for the Incompatible Insect Technique

Hidayanti

Gazali

Tagami

2022

Journal of Insect Science

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“…In particular, IIT may be too expensive if flies are raised on plants rather than on artificial media, and an efficient sex sorting system would need to be developed. Sultan et al [ 69 ] looked at mechanical sorting of puparia of L. trifolii but this proved difficult, and a genetic sexing method may be required. IIT would likely be restricted to greenhouses initially given costs.…”

Section: Discussionmentioning

confidence: 99%

High Incidence of Related Wolbachia across Unrelated Leaf-Mining Diptera

Ridland

Umina

et al. 2021

Insects

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The maternally inherited endosymbiont, Wolbachia pipientis, plays an important role in the ecology and evolution of many of its hosts by affecting host reproduction and fitness. Here, we investigated 13 dipteran leaf-mining species to characterize Wolbachia infections and the potential for this endosymbiont in biocontrol. Wolbachia infections were present in 12 species, including 10 species where the Wolbachia infection was at or near fixation. A comparison of Wolbachia relatedness based on the wsp/MLST gene set showed that unrelated leaf-mining species often shared similar Wolbachia, suggesting common horizontal transfer. We established a colony of Liriomyza brassicae and found adult Wolbachia density was stable; although Wolbachia density differed between the sexes, with females having a 20-fold higher density than males. Wolbachia density increased during L. brassicae development, with higher densities in pupae than larvae. We removed Wolbachia using tetracycline and performed reciprocal crosses between Wolbachia-infected and uninfected individuals. Cured females crossed with infected males failed to produce offspring, indicating that Wolbachia induced complete cytoplasmic incompatibility in L. brassicae. The results highlight the potential of Wolbachia to suppress Liriomyza pests based on approaches such as the incompatible insect technique, where infected males are released into populations lacking Wolbachia or with a different incompatible infection.

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“…The ability to impair male reproductive organ development and/or reverse XX females to phenotypic males that can mate but remain infertile is highly advantageous for insect biological control programmes, such as those using the sterile insect technique (SIT). Studies indicate that SIT, which relies on the production and release of sufficient sterile males to induce sterility in the wild females, could be very efficient for suppressing insect populations (Schliekelman et al, 2005;Lees et al, 2015;Sultan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effect of parental RNA interference of a transformer‐2 homologue on female reproduction and offspring sex determination in Asian citrus psyllid

Killiny

2017

Physiol. Entomol

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Abstract. Asian citrus psyllid Diaphorina citri Kuwayama is the vector of CandidatusLiberibacter asiaticus (CLas), the causal agent of citrus huanglongbing (HLB). A comprehensive understanding of molecular components that govern key physiological traits in D. citri would promote the development of novel genetic control strategies to fight this pest. In the present study, a transformer 2 homologue (Dctra-2) implicated in embryonic sex differentiation in dipteran insects is targeted to interfere with the offspring sex ratio of D. citri. The results of the present study show that Dctra-2 is constitutively expressed at all developmental stages and in both females and males during the adult stage. Quantitative reverse transcriptase-polymerase chain reaction analysis confirms the double-stranded RNA-mediated RNA interference (RNAi) of the Dctra-2. RNAi effects in D. citri female adults can be transferred in a trans-generational manner to their eggs and progeny, which are commonly referred as parental RNAi. RNAi females lay fewer eggs, with a mean of 12.44, and the hatching rate of laid eggs is reduced approximately by 29.86% compared with the control. However, parental RNAi of Dctra-2 do not produce male-biased progeny. The results suggest that Dctra-2 is an effective RNAi target for use in the management of D. citri. In addition, the parental RNAi would provide an effective tool for studying the function of early embryonic genes in D. citri.

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Development of a mechanical sexing system to improve the efficacy of an area‐wide sterile insect release programme to control American serpentine leafminer (Diptera: Agromyzidae) in Canadian ornamental greenhouses

Abstract: Pupal sexual dimorphisms identified in this research can be used to design a sieve to aid in reducing the proportion of females prior to irradiation, thus improving the efficacy of an area-wide sterile insect release programme. © 2016 Society of Chemical Industry.

Cited by 3 publications

References 27 publications

Effect of Quorum Sensing Inducers and Inhibitors on Cytoplasmic Incompatibility Induced byWolbachia(Rickettsiales: Anaplasmataceae) in American Serpentine Leafminer (Diptera: Agromyzidae): Potential Tool for the Incompatible Insect Technique

Effect of Quorum Sensing Inducers and Inhibitors on Cytoplasmic Incompatibility Induced byWolbachia(Rickettsiales: Anaplasmataceae) in American Serpentine Leafminer (Diptera: Agromyzidae): Potential Tool for the Incompatible Insect Technique

High Incidence of Related Wolbachia across Unrelated Leaf-Mining Diptera

Effect of parental RNA interference of a transformer‐2 homologue on female reproduction and offspring sex determination in Asian citrus psyllid

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