Development of a magnetic solid phase extraction method for gemcitabine from rat plasma by using magnetic graphene oxide calix[6]arene composite and its application to pharmacokinetics

Palakeeti, Babji; Babu, Namburi L A Amara; Chinta, Jugun Prakash

doi:10.1080/01496395.2023.2201392

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…A single protein precipitation step was chosen as it was sufficient for sample treatment, conferring it an advantage over more complicated and time-consuming procedures like liquid–liquid extraction or solid-phase extraction [ 27 , 30 ]. The protein precipitation procedure has also been applied in several methods using either organic solvent [ 7 , 21 , 22 , 23 , 25 ] or TCA [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Simple and Reliable HPLC-UV Method for Determining Gemcitabine Levels: Application in Pharmacokinetic Analysis

Lafazanis,

Begas,

Papapostolou

et al. 2024

Medicina

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Background and Objectives: Gemcitabine has been used to treat various solid cancers, including, since 1997, metastatic pancreatic cancer. Here, we developed an HPLC-UV method to determine serum gemcitabine levels and use it in pharmacokinetic studies. Materials and Methods: The analysis was performed after a single protein precipitation step on a reversed-phase column, isocratically eluted with sodium phosphate buffer and methanol. For the pharmacokinetic study, NOD/SCID mice received a single dose of gemcitabine at 100 mg/kg by either subcutaneous (SC) or intraperitoneal (IP) administration. Blood samples were collected at 5, 15, and 30 min and 1, 2, 4, and 6 h after the administration of gemcitabine for further analysis. Results: The duration of the analysis was ~12.5 min. The calibration curve was linear (r2 = 0.999) over the range of 1–400 μM. The mean recovery of GEM was 96.53% and the limit of detection was 0.166 μΜ. T1/2, Tmax, Cmax, AUC0–t, and clearance were 64.49 min, 5.00 min, 264.88 μmol/L, 9351.95 μmol/L*min, and 0.0103(mg)/(μmol/L)/min, respectively, for the SC administration. The corresponding values for the IP administration were 59.34 min, 5.00 min, 300.73 μmol/L, 8981.35 μmol/L*min and 0.0108(mg)/(μmol/L)/min (not statistically different from the SC administration). Conclusions: A simple, valid, sensitive, and inexpensive method for the measurement of gemcitabine in serum has been developed. This method may be useful for monitoring gemcitabine levels in cancer patients as part of therapeutic drug monitoring.

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Section: Discussionmentioning

confidence: 99%

Development and Validation of a Simple and Reliable HPLC-UV Method for Determining Gemcitabine Levels: Application in Pharmacokinetic Analysis

Lafazanis,

Begas,

Papapostolou

et al. 2024

Medicina

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“…The upper and lower edges can be embellished by various functional groups to prepare hydro-soluble calixarene for the extraction of various organic cationic compounds such as alkaloids through electrostatic interactions [87]. A novel magnetic graphene oxide-based calixarene (MGO-C[6]A) composite was prepared and served as an adsorbent for MSPE of gemcitabine from rat plasma by Palakeeti et al [88]. MGO-C [6]A composite can be used as a trapping agent to encapsulate the target molecules through host-guest interactions under the optimized conditions, linearity was evaluated with R 2 = 0.9993).…”

Section: Analysis Of Phytochemicals In Biological Samplesmentioning

confidence: 99%

Applications of magnetic solid‐phase extraction in the sample preparation of natural product analysis (2020–2023)

Chen,

Yang

2024

J of Separation Science

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Sample preparation, including extraction, separation, and purification, is a vital process for natural product analysis. As an attractive sample pretreatment method, magnetic solid‐phase extraction (MSPE) has gained plenty of attention, mainly due to its simpler operation, less consumption of organic solvents, and shorter processing time than traditional SPE. This updated review is devoted to summarizing the applications of MSPE based on different magnetic nanomaterials in the analysis of various natural products in complex matrixes, such as biological samples, plants, and Chinese herbal preparations in the past four years (2020–2023). The preparation and fabrication of different materials are briefly introduced. Furthermore, the extraction mechanism and interaction forces between adsorbent and analytes are elaborated, and the advantages and disadvantages of different adsorbents coupled with various analytical methods for MSPE of different natural products are summarized. Moreover, the future trends and opportunities for MSPE in the natural product analysis are discussed. It is expected that this work can provide updated information for future research on the applications of MSPE in such fields.

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Development of a magnetic solid phase extraction method for gemcitabine from rat plasma by using magnetic graphene oxide calix[6]arene composite and its application to pharmacokinetics

Cited by 2 publications

References 38 publications

Development and Validation of a Simple and Reliable HPLC-UV Method for Determining Gemcitabine Levels: Application in Pharmacokinetic Analysis

Development and Validation of a Simple and Reliable HPLC-UV Method for Determining Gemcitabine Levels: Application in Pharmacokinetic Analysis

Applications of magnetic solid‐phase extraction in the sample preparation of natural product analysis (2020–2023)

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