Development of a low-pressure diamond anvil cell and analytical tools to monitor microbial activities in situ under controlled P and T

Oger, Philippe; Daniel, Isabelle; Picard, Aude

doi:10.1016/j.bbapap.2005.11.009

Cited by 31 publications

(22 citation statements)

References 52 publications

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“…It took 2 to 3 s for the gas pressure to reach the target value when the controller changed the gas membrane pressure. The DAC pressure was calibrated using standard ruby fluorescence and pressure-sensitive fluorescent polystyrene microspheres (FluoSpheres F-2111; Invitrogen Life Technologies, Grand Island, NY) (32). The accuracy for the pressure calibration was ϳ10 MPa.…”

Section: Methodsmentioning

confidence: 99%

Monitoring Rates and Heterogeneity of High-Pressure Germination of Bacillus Spores by Phase-Contrast Microscopy of Individual Spores

Kong

Doona

Setlow

et al. 2014

Appl Environ Microbiol

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Germination of Bacillus spores with a high pressure (HP) of ϳ150 MPa is via activation of spores' germinant receptors (GRs). The HP germination of multiple individual Bacillus subtilis spores in a diamond anvil cell (DAC) was monitored with phasecontrast microscopy. Major conclusions were that (i) >95% of wild-type spores germinated in 40 min in a DAC at ϳ150 MPa and 37°C but individual spores' germination kinetics were heterogeneous; (ii) individual spores' HP germination kinetic parameters were similar to those of nutrient-triggered germination with a variable lag time (T lag ) prior to a period of the rapid release (⌬T release ) of the spores' dipicolinic acid in a 1:1 chelate with Ca 2؉ (CaDPA); (iii) spore germination at 50 MPa had longer average T lag values than that at ϳ150 MPa, but the ⌬T release values at the two pressures were identical and HPs of <10 MPa did not induce germination; (iv) B. subtilis spores that lacked the cortex-lytic enzyme CwlJ and that were germinated with an HP of 150 MPa exhibited average ⌬T release values ϳ15-fold longer than those for wild-type spores, but the two types of spores exhibited similar average T lag values; and (v) the germination of wild-type spores given a >30-s 140-MPa HP pulse followed by a constant pressure of 1 MPa was the same as that of spores exposed to a constant pressure of 140 MPa that was continued for >35 min; (vi) however, after short 150-MPa HP pulses and incubation at 0.1 MPa (ambient pressure), spore germination stopped 5 to 10 min after the HP was released. These results suggest that an HP of ϳ150 MPa for <30 s is sufficient to fully activate spores' GRs, which remain activated at 1 MPa but can deactivate at ambient pressure.

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Section: Methodsmentioning

confidence: 99%

Monitoring Rates and Heterogeneity of High-Pressure Germination of Bacillus Spores by Phase-Contrast Microscopy of Individual Spores

Kong

Doona

Setlow

et al. 2014

Appl Environ Microbiol

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“…Over the past five years, specific developments aimed at adapting the DAC to XRF geometry (detector at 90 degrees). This optimal angle of detection may either be obtained by using a partly drilled diamond [58] or by replacing one anvil by a diamond window and using an X-ray transparent gasket material [59]. Such improvements have allowed detection limits down to 2 ppm, at the expenses of weaker mechanical properties, which reduce the maximum pressure to 2 GPa.…”

Section: Chemical Composition and Statementioning

confidence: 98%

Advances in synchrotron hard X-ray based imaging

Baruchel

Bleuet

Bravin

et al. 2007

Comptes Rendus. Physique

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“…Our researches for a suitable DAC for SAXS experiments under moderate pressure led us to get interested on a recently developed DAC for the study of biological systems with Raman spectroscopy under pressure. 21 Contrarily to classical DAC in which we find two identical opposite diamond anvils, this cell is designed with a single diamond anvil with a culet diameter of 1.4 mm. The second anvil is replaced by a flat diamond window 600 µm thick.…”

Section: The High Pressure Cell For Saxs In Solidsmentioning

confidence: 99%

Small angle scattering methods to study porous materials under high uniaxial strain

Floch

Balima

Pischedda

et al. 2015

Review of Scientific Instruments

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We developed a high pressure cell for the in situ study of the porosity of solids under high uniaxial strain using neutron small angle scattering. The cell comprises a hydraulically actioned piston and a main body equipped with two single-crystal sapphire windows allowing for the neutron scattering of the sample. The sample cavity is designed to allow for a large volume variation as expected when compressing highly porous materials. We also implemented a loading protocol to adapt an existing diamond anvil cell for the study of porous materials by X-ray small angle scattering under high pressure. The two techniques are complementary as the radiation beam and the applied pressure are in one case perpendicular to each other (neutron cell) and in the other case parallel (X-ray cell). We will illustrate the use of these two techniques in the study of lamellar porous systems up to a maximum pressure of 0.1 GPa and 0.3 GPa for the neutron and X-ray cells, respectively. These devices allow obtaining information on the evolution of porosity with pressure in the pore dimension subdomain defined by the wave-numbers explored in the scattering process. The evolution with the applied load of such parameters as the fractal dimension of the pore-matrix interface or the apparent specific surface in expanded graphite and in expanded vermiculite is used to illustrate the use of the high pressure cells.

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Development of a low-pressure diamond anvil cell and analytical tools to monitor microbial activities in situ under controlled P and T

Cited by 31 publications

References 52 publications

Monitoring Rates and Heterogeneity of High-Pressure Germination of Bacillus Spores by Phase-Contrast Microscopy of Individual Spores

Monitoring Rates and Heterogeneity of High-Pressure Germination of Bacillus Spores by Phase-Contrast Microscopy of Individual Spores

Advances in synchrotron hard X-ray based imaging

Small angle scattering methods to study porous materials under high uniaxial strain

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