Development of a low cost gas diffusion device for ammonia detection in the vitreous humor and its preliminary application for estimation of the time since death

Musile, Giacomo; Gottardo, Rossella; Palacio, Covadonga; Shestakova, Ksenia; Raniero, Dario; Palo, Elio Franco De; Tagliaro, Franco

doi:10.1016/j.forsciint.2018.12.012

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…Ammonia evaporation is promoted by the basic pH solution; since the vial is a closed system, external disturbances to the liquid-gas interface are prevented. The device design resembled a previously described unit used for detecting ammonia in vitreous humor samples [39]. The method exploits a colorimetric reaction between molecular ammonia and the Nessler's reagent (Equation (1)).…”

Section: Fabrication Of the Gas Diffusion Devicementioning

confidence: 99%

Chitosan Film Sensor for Ammonia Detection in Microdiffusion Analytical Devices

Tagliaro,

Musile,

Caricato

et al. 2023

Polymers

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Chitosan films have attracted increased attention in the field of sensors because of chitosan’s unique chemico-physical properties, including high adsorption capacity, filmability and transparency. A chitosan film sensor was developed through the dispersion of an ammonia specific reagent (Nessler’s reagent) into a chitosan film matrix. The chitosan film sensor was characterized to assess the film’s properties by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). A gas diffusion device was prepared with the chitosan film sensor, enabling the collection and detection of ammonia vapor from biological samples. The chitosan film sensor color change was correlated with the ammonia concentration in samples of human serum and artificial urine. This method enabled facile ammonia detection and concentration measurement, making the sensor useful not only in clinical laboratories, but also for point-of-care devices and wherever there is limited access to modern laboratory facilities.

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Section: Fabrication Of the Gas Diffusion Devicementioning

confidence: 99%

Chitosan Film Sensor for Ammonia Detection in Microdiffusion Analytical Devices

Tagliaro,

Musile,

Caricato

et al. 2023

Polymers

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“…The drawback of this method is that it is susceptible to pH and other volatile amines. 29,30 To solve this pH interference problem, Flood et al proposed a new method for ion assay by ion-pair formation. Stromberg 31 introduced the NH 4 + selective ionophore nonactin for the NH 4 + assay; Jonah et al 32 used pyrazole to accomplish this, they have a common disadvantage, which is the interference of potassium ions.…”

Section: Introductionmentioning

confidence: 99%

“…Another common optical ammonia sensor is designed based on a pH indicator, where ammonia reacts with the pH indicator, and the pH indicator is then deprotonated, resulting in changes in absorption and fluorescence. The drawback of this method is that it is susceptible to pH and other volatile amines 29,30. To solve this pH interference problem, Flood et al…”

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confidence: 99%

Highly selective fluorescent sensor for ammonium ions

Shen,

Pan,

Chen

et al. 2024

Sens. Diagn.

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“…Surprisingly, it was neglected by most of authors for quite obscure reasons, but probably related to well-known analytical problems in clinical chemistry and laboratory medicine. 12–18 To the best of our knowledge, a single paper did appear in 1978 reporting an ammonium increase in the vitreous humour in correlation with the post mortem interval. In this article, a colorimetric method based on the Nessler’s reagent was used, without any separation step.…”

Section: Introductionmentioning

confidence: 99%

“…9 In addition, two more methodologies developed by Musile et al., a gas diffusion device and a microfluidic paper-based device, were recently developed in our research group. 18,19 The aim of both is the determination of NH 4 + in vitreous humour. Both of them are portable and provide in situ qualitative results which will need to be confirmed by CE.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of potassium and ammonium ions in the vitreous humour by capillary electrophoresis and their integrated use to infer the post mortem interval (PMI)

Palacio

Gottardo

Cirielli³

et al. 2020

Med Sci Law

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Post-mortem changes of ions in the body fluids have been proposed as an objective tool for inferring the time of death. In particular, the post-mortem increase of potassium concentrations in the vitreous humour has gained great attention in the literature. On the other hand, ammonium, another ion released in post-mortem processes, has received much less attention, potentially due to unresolved analytical issues using current clinical chemistry methods. This paper presents an application of a new analytical approach based on capillary electrophoresis providing the simultaneous analysis of potassium and ammonium ions in the vitreous humour. In addition, to assess the consistency of the post-mortem increase of ammonium concentrations in the vitreous humour, the determination of this ion in the vitreous humour of the two eyes of the same body at the same post-mortem interval has been verified. Vitreous humour was collected from 33 medico-legal cases where the time of death was known exactly. Prior to analysis, all samples were diluted 1:20 with a 40 μg/mL solution of BaCl2 (internal standard). In the study of the variability of the ammonium concentration between the two eyes, no statistically significant differences were found, supporting the hypothesis of an even post-mortem increase of the ion concentrations in this particular biological fluid. Significant correlations of potassium and ammonium ions with the post-mortem interval were found, with r2 of 0.75 and 0.70, respectively.

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Development of a low cost gas diffusion device for ammonia detection in the vitreous humor and its preliminary application for estimation of the time since death

Cited by 7 publications

References 21 publications

Chitosan Film Sensor for Ammonia Detection in Microdiffusion Analytical Devices

Chitosan Film Sensor for Ammonia Detection in Microdiffusion Analytical Devices

Highly selective fluorescent sensor for ammonium ions

Simultaneous analysis of potassium and ammonium ions in the vitreous humour by capillary electrophoresis and their integrated use to infer the post mortem interval (PMI)

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