Development of a loop-mediated isothermal amplification (LAMP) assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses

Hifumi, Tatsuro; Akioka, Kohei; Tanaka, Tetsuya; Miyoshi, Noriaki

doi:10.1016/j.vetpar.2021.109573

Cited by 3 publications

(1 citation statement)

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“…In terms of specificity, it had no cross reaction with other parasites, including bovine cysticercus, Clonorchis sinensis, and Schistosoma (Ahmed et al, 2016). Moreover, there was also the development of a LAMP assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses (Hifumi et al, 2021). Compared with other methods, the DNA detection method of E. multilocularis and E. granulosus based on the LAMP can be used for rapid and early diagnosis of parasitic infection in a large range, with low cost and high popularization significance.…”

Section: Loop-mediated Isothermal Amplification (Lamp)mentioning

confidence: 99%

Advances in the study of molecular identification technology of Echinococcus species

R.J.¹,

J.Z.²

2022

Trop Biomed

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The larvae of Echinococcus (hydatidcyst) can parasitize humans and animals, causing a serious zoonotic disease-echinococcosis. The life history of Echinococcus is complicated, and as the disease progresses slowly after infection, early diagnosis is difficult to establish. Due to the limitations of imaging and immunological diagnosis in this respect, domestic and foreign scholars have established a variety of molecular detection techniques for the pathogen Echinococcus over recent years, mainly including nested polymerase chain reaction (PCR), multiplex PCR, real-time quantitative PCR, and nucleic acid isothermal amplification technology. In this article, the research progress of molecular detection technology for Echinococcus infection currently was reviewed and the significance of these methods in the detection and diagnosis of hydatid and hydatid diseases was also discussed.

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Section: Loop-mediated Isothermal Amplification (Lamp)mentioning

confidence: 99%