Development of a loop-mediated isothermal amplification (LAMP) and a direct LAMP for the specific detection of Nosema ceranae, a parasite of honey bees

Lannutti, Lucas; Mira, Anabela; Basualdo, Marina; Rodríguez, Graciela; Erler, Silvio; Silva, Victoria; Gisder, Sebastian; Genersch, Elke; Florín-Christensen, Mónica; Schnittger, Leonhard

doi:10.1007/s00436-020-06915-w

Cited by 17 publications

(15 citation statements)

References 68 publications

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“…In the last years, several research groups have been working on improving different molecular techniques. Lannutti et al (2020) developed loop-mediated isothermal amplification (LAMP) for detection of N. ceranae [ 91 ]. The same year, Ribani et al confirmed that environmental DNA analysis in honey could be a useful tool for detecting N. ceranae [ 91 ].…”

Section: Detection and Outcome Of N Ceranae Infectionmentioning

confidence: 99%

“…Lannutti et al (2020) developed loop-mediated isothermal amplification (LAMP) for detection of N. ceranae [ 91 ]. The same year, Ribani et al confirmed that environmental DNA analysis in honey could be a useful tool for detecting N. ceranae [ 91 ]. Alternatively, different genetic variants of N. ceranae trigger different immune responses in the host [ 92 ], making diagnosis even more difficult.…”

Section: Detection and Outcome Of N Ceranae Infectionmentioning

confidence: 99%

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The Role of Nosema ceranae (Microsporidia: Nosematidae) in Honey Bee Colony Losses and Current Insights on Treatment

Marín-García

Peyre

Ahuir-Baraja

et al. 2022

Veterinary Sciences

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Honeybee populations have locally and temporally declined in the last few years because of both biotic and abiotic factors. Among the latter, one of the most important reasons is infection by the microsporidia Nosema ceranae, which is the etiological agent of type C nosemosis. This species was first described in Asian honeybees (Apis cerana). Nowadays, domestic honeybees (Apis mellifera) worldwide are also becoming infected due to globalization. Type C nosemosis can be asymptomatic or can cause important damage to bees, such as changes in temporal polyethism, energy and oxidative stress, immunity loss, and decreased average life expectancy. It causes drastic reductions in workers, numbers of broods, and honey production, finally leading to colony loss. Common treatment is based on fumagillin, an antibiotic with side effects and relatively poor efficiency, which is banned in the European Union. Natural products, probiotics, food supplements, nutraceuticals, and other veterinary drugs are currently under study and might represent alternative treatments. Prophylaxis and management of affected colonies are essential to control the disease. While N. ceranae is one potential cause of bee losses in a colony, other factors must also be considered, especially synergies between microsporidia and the use of insecticides.

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Section: Detection and Outcome Of N Ceranae Infectionmentioning

confidence: 99%

Section: Detection and Outcome Of N Ceranae Infectionmentioning

confidence: 99%

The Role of Nosema ceranae (Microsporidia: Nosematidae) in Honey Bee Colony Losses and Current Insights on Treatment

Marín-García

Peyre

Ahuir-Baraja

et al. 2022

Veterinary Sciences

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“…In many parts of the world, such as the European Union, antibiotics for Nosemosis treatment are banned. There have been three separate LAMP assays developed to detect N. ceranae and one for the detection of N. apis (Table 1) (42)(43)(44). Also, one has been developed for N. bombi infection within wild bumble bee populations (45).…”

Section: Application Of Lamp For the Detection Of Fungal Pathogens Of Honey Beesmentioning

confidence: 99%

“…Also, one has been developed for N. bombi infection within wild bumble bee populations (45). All three of the N. ceranae LAMP assays are extremely sensitive for the detection of infection; however, the LAMP assay developed by Lannutti et al (44) uses primers targeting the polar tube protein 3 (PTP3) gene, a highly specific and conserved gene of N. ceranae. Targeting the PTP3 gene overcomes non-specific amplification that can occur due to polymorphisms in the 16S rRNA gene, which is the other two assays' target gene (55).…”

Section: Application Of Lamp For the Detection Of Fungal Pathogens Of Honey Beesmentioning

confidence: 99%

“…Targeting the PTP3 gene overcomes non-specific amplification that can occur due to polymorphisms in the 16S rRNA gene, which is the other two assays' target gene (55). Furthermore, only the PTP3-LAMP assay has been successfully evaluated using a panel of field samples (44). Two brood diseases, stonebrood and chalkbrood, are caused by the fungal species Aspergillus flavus and Ascophaera apis, respectively.…”

Section: Application Of Lamp For the Detection Of Fungal Pathogens Of Honey Beesmentioning

confidence: 99%

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Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens

2021

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Approximately one-third of the typical human Western diet depends upon pollination for production, and honey bees (Apis mellifera) are the primary pollinators of numerous food crops, including fruits, nuts, vegetables, and oilseeds. Regional large scale losses of managed honey bee populations have increased significantly during the last decade. In particular, asymptomatic infection of honey bees with viruses and bacterial pathogens are quite common, and co-pathogenic interaction with other pathogens have led to more severe and frequent colony losses. Other multiple environmental stress factors, including agrochemical exposure, lack of quality forage, and reduced habitat, have all contributed to the considerable negative impact upon bee health. The ability to accurately diagnose diseases early could likely lead to better management and treatment strategies. While many molecular diagnostic tests such as real-time PCR and MALDI-TOF mass spectrometry have been developed to detect honey bee pathogens, they are not field-deployable and thus cannot support local apiary husbandry decision-making for disease control. Here we review the field-deployable technology termed loop-mediated isothermal amplification (LAMP) and its application to diagnose honey bee infections.

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Development of a highly specific LAMP assay for detection of Sarcocystis tenella and Sarcocystis gigantea in sheep

Chen,

Peng,

Zhu

et al. 2024

Parasitol Res

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Development of a loop-mediated isothermal amplification (LAMP) and a direct LAMP for the specific detection of Nosema ceranae, a parasite of honey bees

Cited by 17 publications

References 68 publications

The Role of Nosema ceranae (Microsporidia: Nosematidae) in Honey Bee Colony Losses and Current Insights on Treatment

The Role of Nosema ceranae (Microsporidia: Nosematidae) in Honey Bee Colony Losses and Current Insights on Treatment

Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens

Development of a highly specific LAMP assay for detection of Sarcocystis tenella and Sarcocystis gigantea in sheep

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