Development of a Liquid-Junction/Low-Flow Interface for Phosphate Buffer Capillary Electrophoresis Mass Spectrometry

Li, Fuan; Huang, Ju-Li; Shen, Shang-Yu; Wang, Che-Wei; Her, Guor‐Rong

doi:10.1021/ac802491y

Cited by 24 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(A)) consisted of a poly(methyl methacrylate)‐based liquid reservoir and a tapered tip as the ESI sprayer. A 1.5 cm × 700 μm id × 850 μm od fused‐silica capillary was tapered to produce a 5 μm orifice according to the procedure reported previously . The fused silica was drawn manually using a vertically suspended section of capillary to which a small weight (45 g) had been attached.…”

Section: Methodsmentioning

confidence: 99%

A convenient and sensitive method for haloacetic acid analysis in tap water by on-line field-amplified sample-stacking CE-ESI-MS

Hung

Her

2013

J. Sep. Science

Self Cite

View full text Add to dashboard Cite

In this study, we propose a simple strategy based on flow injection and field-amplified sample-stacking CE-ESI-MS/MS to analyze haloacetic acids (HAAs) in tap water. Tap water was passed through a desalination cartridge before field-amplified sample-stacking CE-ESI-MS/MS analysis to reduce sample salinity. With this treatment, the signals of the HAAs increased 300- to 1400-fold. The LODs for tap water analysis were in the range of 10 to 100 ng/L, except for the LOD of monochloroacetic acid (1 μg/L in selected-ion monitoring mode detection). The proposed method is fast, convenient, and sensitive enough to perform on-line analysis of five HAAs in the tap water of Taipei City. Four HAAs, including trichloroacetic acid, dichloroacetic acid, dibromoacetic acid, and monobromoacetic acid, were detected at concentrations of approximately 1.74, 1.15, 0.16, and 0.15 ppb, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

A convenient and sensitive method for haloacetic acid analysis in tap water by on-line field-amplified sample-stacking CE-ESI-MS

Hung

Her

2013

J. Sep. Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In CE, to reduce ion suppression due to the use of phosphate ions, a L–J interface was also used being phosphate anions moving in the direction of the inlet capillary. When using additives such as sodium dodecyl sulphate, good results were obtained allowing the micelles to be eliminated into the liquid‐junction .…”

Section: Hephenation With Mass Spectrometrymentioning

confidence: 99%

An overview to nano‐scale analytical techniques: Nano‐liquid chromatography and capillary electrochromatography

Fanali

2017

Electrophoresis

View full text Add to dashboard Cite

Nano-liquid chromatography (nano-LC) and CEC are microfluidic techniques mainly used for analytical purposes. They have been applied to the separation and analysis of a large number of compounds, e.g., peptides, proteins, drugs, enantiomers, antibiotics, pesticides, nutraceutical, etc. Analytes separation is carried out into capillaries containing selected stationary phase. The mobile phase is moved either by a pump (nano-LC) or by an EOF, respectively. The two tools can offer some advantages over conventional techniques, e.g., high selectivity, separation efficiency, resolution, short analysis time and consumption of low volumes of mobile phase. Flow rates in the range 50-800 nL/min are usually applied. The low flow rate reduces the chromatographic dilution increasing the mass sensitivity. Special attention must be paid in avoiding peak dispersion selecting the appropriate detector, injector and tube connection. Finally due to the low flow rate these microfluidic techniques can be easily coupled with mass spectrometry.

show abstract

“…Notably, it could also be concluded that the new interface could run with nonvolatile PBS directly, which is a traditional biological buffer widely used in biochemistry studies, without any additives such as methanol and acetic acid. [31][32][33] This might expand the applicable range of buffers for the current CE-MS interface.…”

Section: Ce Separation With the Ce-iesi-ms Interfacementioning

confidence: 99%

Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular‐sized capillary

Yin

Guan

et al. 2016

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

RATIONALE:The flow rate match has been a great challenge when coupling capillary electrophoresis (CE) with electrospray ionization mass spectrometry (ESI-MS). Conventional CE-ESI-MS interfaces used liquid sheath flow, narrowed capillary or additional pressure to meet this requirement; sacrifice of either capillary inner diameter (i.d.) or separation efficiency is often inevitable. Thus, a regular-sized capillary-based sheathless interface would be attractive for flow rate match in CE-MS. METHODS:The regular-sized capillary-based CE-MS interface was achieved by coupling CE with induced electrospray ionization (iESI) which was stimulated by the fact that the iESI could both achieve flow rate down to 0.2 μL/min and retain ionization efficiency. The CE-iESI-MS interface was completed with an intact separation capillary, outside the outlet end of which a metal electrode was attached for the application of alternating current (ac) high voltage (HV). RESULTS: The feasibility of this CE-iESI-MS interface was demonstrated through the stable total ion chromatograms obtained by continuous CE infusion of tripropylamine with regular-sized capillaries. Tripropylamine and atenolol were separated and detected successfully in phosphate buffer solution (PBS) by CE-iESI-MS using a 50 or 75 μm i.d. capillary. Furthermore, this new interface showed a better signal-to-noise (S/N) of 3 to 7 times enhancement compared with another sheathless CE-ESI-MS interface that using one high voltage for both separation and electrospray when analyzing the mixture of tripropylamine and proline in NH 4 OAc buffer. In addition, the reproducibility of this interface gave satisfactory results with relative standard deviation (RSD) in retention time in the range between 1% and 3%. CONCLUSIONS: The novel sheathless CE-MS interface introduced here could match conventional electroosmotic flow (EOF) with electrospray which could also preserve the separation efficiency and sensitivity of CE-MS. This newly developed CE-iESI-MS interface was also demonstrated to be effective for different buffers, PBS and NH 4 OAc, without any additives such as methanol and acetic acid. Hence, we believe that this sheathless CE-MS interface could be operated with other nonvolatile and volatile buffers. Copyright © 2016 John Wiley & Sons, Ltd.The combination of capillary electrophoresis (CE) and mass spectrometry (MS) [1][2][3] has become a powerful technique for separation and detection of a broad range of compounds since it was introduced in the late 1980s. [4] To date, electrospray ionization (ESI) has been the most frequently used interface for CE-MS coupling. However, there are two major challenges when interfacing CE with ESI-MS. One is to maintain a constant electrical contact for both CE separation and the ESI process. A sheath or make-up flow [5][6][7] can be used to mix with the CE effluent at the outlet to establish the electrical contact. While for sheathless interfaces the electrical contact was established by direct application of high voltage (HV) via a conductiv...

show abstract

Development of a Liquid-Junction/Low-Flow Interface for Phosphate Buffer Capillary Electrophoresis Mass Spectrometry

Cited by 24 publications

References 28 publications

A convenient and sensitive method for haloacetic acid analysis in tap water by on-line field-amplified sample-stacking CE-ESI-MS

A convenient and sensitive method for haloacetic acid analysis in tap water by on-line field-amplified sample-stacking CE-ESI-MS

An overview to nano‐scale analytical techniques: Nano‐liquid chromatography and capillary electrochromatography

Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular‐sized capillary

Contact Info

Product

Resources

About