Development of a liquid chromatography-tandem mass spectrometry with pressurized liquid extraction for determination of glucocorticoid residues in edible tissues

Chen, Dongmei; Tao, Yanfei; Liu, Zhaoying; Zhang, Huahai; Liu, Zhenli; Wang, Yulian; Huang, Lingli; Pan, Yuanhu; Peng, Dapeng; Dai, Menghong; Wang, Xu; Zhang, Yuan

doi:10.1016/j.jchromb.2010.11.039

Cited by 32 publications

(7 citation statements)

References 26 publications

(27 reference statements)

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“…To illustrate the advantages of this work as a promising extraction strategy, we compared our optimized method with other reported method [10,12,16,18,24,25,[30][31][32][33][34] . The LOD values obtained by the proposed method are in the range of 0.01-0.05 μg kg À 1 , which is obviously comparable or even better than that of other conventional methods.…”

Section: Methods Validationmentioning

confidence: 99%

Microwave-assisted enzymatic hydrolysis followed by extraction with restricted access nanocomposites for rapid analysis of glucocorticoids residues in liver tissue

Feng

Liu

et al. 2016

Talanta

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Section: Methods Validationmentioning

confidence: 99%

Microwave-assisted enzymatic hydrolysis followed by extraction with restricted access nanocomposites for rapid analysis of glucocorticoids residues in liver tissue

Feng

Liu

et al. 2016

Talanta

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“…It is used to suppress the immune response (Pyka, Babuska-Roczniak, & Bochenska, 2011) and promote weight gain in animals by reducing energy consumption (Quillet et al, 2014). However, its overuse causes residues to accumulate in animal products, such as milk and pork, which can endanger human health (Chen et al, 2011). Therefore, there is an urgent need to develop methods to monitor HDS residues in animal products.…”

Section: Introductionmentioning

confidence: 99%

Development of an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic assay for hydrocortisone residues in milk

Wang

Xie

Cui

et al. 2017

Food and Agricultural Immunology

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An anti-hydrocortisone (HDS) monoclonal antibody, 2G8, based on a HDS succinic anhydride derivative hapten, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic assay for the detection of HDS in milk samples. The half inhibitory concentration (IC 50) of the antibody was 0.095 ng/mL, its limit of detection was 0.013 ng/mL, its linear range of detection was 0.026-0.356 ng/mL, and its cross-reactivity with HDS analogs was <5%. In spiked samples and a recovery test, the recovery rates ranged from 92% to 98.5%, indicating the suitability of this ic-ELISA for the analysis of HDS in milk. The immunochromatographic strip had a cutoff value of 2 ng/mL in milk and could be used for the semiquantitative analysis of HDS. When milk samples were added to the sample pad of the strip, a bright test line indicated <0.2 ng/mL HDS, a weak test line indicated 0.2-2 ng/mL HDS, and no test line indicated ≥2 ng/mL HDS. Analysis of HDS in milk samples showed that results acquired by the immunochromatographic assay agreed well with results acquired by ic-ELISA. Thus, the ic-ELISA and strip assay developed in this study rapidly and sensitively detect HDS residues in milk samples.

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“…Recently, various analytical methods have been developed for detecting DEX, such as high-performance liquid chromatography (Bhargava et al, 2016;Dési, Kovács, Palotai, & Kende, 2008;Lasić, Bobarević, & Nikolin, 1989;Tsuei, Ashley, Moore, & McBride, 1978), gas chromatography-mass spectrometry (Amendola, Garribba, & Botrè, 2003;Bagnati et al, 1996;Huetos Hidalgo, Jiménez López, Ajenjo Carazo, San Andrés Larrea, & Reuvers, 2003), liquid chromatography-mass spectrometry (LC-MS) (Chen et al, 2011;Creaser, Feely, Houghton, Seymour, & Teale, 1996), radioimmunoassay methods (Meikle, Lagerquist, & Tyler, 1973), and enzyme immunoassay (Hassan, Rowell, Hambleton, & Jackson, 1998;Vdovenko, Gribas, Vylegzhanina, & Sakharov, 2012;Yadav et al, 2013;Yoshino, Yoshiharu, Noriko, Kiyoshi, & Fukuko, 1992). Each method has its own strong and weak points.…”

Section: Introductionmentioning

confidence: 99%

Preparation of an anti-dexamethasone monoclonal antibody and its use in development of a colloidal gold immunoassay

Wang

Zheng

Guo

et al. 2017

Food and Agricultural Immunology

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A lateral flow colloidal gold (CG) immunoassay strip has been developed for detection of dexamethasone (DEX) residues in milk samples. For this purpose, an anti-DEX monoclonal antibody (McAb), based on a DEX succinic anhydride derivative hapten, was prepared and characterized. The McAb showed a high specificity to DEX, the half inhibitory concentration of the antibody was 0.095 ng/mL, its limit of detection (LOD) was 0.017 ng/mL, and its linear range of detection was 0.034-0.265 ng/mL. The developed CG immunoassay had a visual cutoff value of 0.3 ng/mL in phosphate buffered saline (PBS) and 0.5 ng/mL in milk samples. Each test requires 10 min. Analysis of DEX in milk indicated that the results of strip assay had a strong agreement with indirect competitive enzyme-linked immunosorbent assay. Therefore, the CG immunoassay is a sensitive screening method for semiquantitative and qualitative detection of DEX residues in milk samples.

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Development of a liquid chromatography-tandem mass spectrometry with pressurized liquid extraction for determination of glucocorticoid residues in edible tissues

Cited by 32 publications

References 26 publications

Microwave-assisted enzymatic hydrolysis followed by extraction with restricted access nanocomposites for rapid analysis of glucocorticoids residues in liver tissue

Microwave-assisted enzymatic hydrolysis followed by extraction with restricted access nanocomposites for rapid analysis of glucocorticoids residues in liver tissue

Development of an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic assay for hydrocortisone residues in milk

Preparation of an anti-dexamethasone monoclonal antibody and its use in development of a colloidal gold immunoassay

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