Development of a lipid-rich, soft plaque in rabbits, monitored by histology and intravascular ultrasound

Chiesa, Giulia; Mario, Carlo Di; Colombo, N.; Vignati, Laila; Marchesi, Matteo; Monteggia, E; Parolini, Cinzia; Lorenzon, Paolo; Laucello, M; Lorusso, Vito; Adamian, Milena; Franceschini, Guido; Newton, Roger S.; Sirtori, Cesare R.

doi:10.1016/s0021-9150(00)00655-9

Cited by 37 publications

(32 citation statements)

References 35 publications

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“…19 This is also the case of animals undergoing infusion of AIM-PL complexes, as assessed by histological examination of 3 animals euthanized without treatment (data not shown).…”

Section: Features Of Arterial Lesionsmentioning

confidence: 83%

“…18 Lipid-rich plaque formation was induced at both common carotid arteries of rabbits by perivascular injury followed by a dietary treatment for 90 days as described. 19 At 90 days after surgery, 5 groups of rabbits, 5 animals each, received one of the following treatments administered through an IVUS catheter inserted into the right carotid 19 : (1) saline; (2) dipalmitoylphosphatidylcholine (DPPC) liposomes (2500 mg); (3) AIM-PL complexes (250 mg proteinϩ625 mg DPPC); (4) AIM-PL complexes (500 mg proteinϩ1250 mg DPPC); and (5) AIM-PL complexes (1000 mg proteinϩ2500 mg DPPC).…”

Section: Experimental Protocolmentioning

confidence: 99%

“…The carotid plaque at maximum stenosis was specifically evaluated, as described, 19 before, during, and at the end of the infusion by 2 independent observers using computerized planimetry (TapeMeasure, Indec Systems). X-ray analysis allowed positioning of the ultrasound probe at the site of maximal stenosis for the following histology.…”

Section: Ivus Imagingmentioning

confidence: 99%

“…compound (BDH Laboratory Supplies); sections were stained as described. 19 AIM detection was performed by using the same anti-human apoA-I antibody used for AIM plasma quantification.…”

Section: Histological Evaluation and Quantitation Of Lesionsmentioning

confidence: 99%

“…19 Adjacent sections were taken at 150-m intervals, within the area corresponding to the maximal plaque accumulation visualized by IVUS. For comparison with the IVUS findings, the atherosclerotic plaque was measured as intima plus media CSA and calculated as external elastic membrane CSA minus the lumen CSA.…”

Section: Histological Evaluation and Quantitation Of Lesionsmentioning

confidence: 99%

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Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Chiesa

Monteggia

Marchesi

et al. 2002

Circulation Research

195

104

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Abstract-Apolipoprotein A-I Milano (AIM), a natural variant of human apolipoprotein A-I, confers to carriers a significant protection against vascular disease. In previous studies, administration of recombinant AIM-phospholipid (AIM-PL) complexes to hypercholesterolemic rabbits markedly inhibited neointimal formation after arterial injury; moreover, repeated injections of AIM-PL in apoE-deficient mice significantly reduced atherosclerosis progression. The objective of the present study was to determine if a single localized infusion of AIM-PL complexes administered directly to atheromatous lesions could promote plaque regression. Lipid-rich, atheromatous plaques were generated at both common carotid arteries of 25 rabbits by applying a perivascular electric injury, followed by 1.5% cholesterol diet for 90 days. Rabbits were infused with either saline, phospholipid vesicles, or 3 different AIM-PL doses (250, 500, or 1000 mg of protein) delivered through an intravascular ultrasound (IVUS) catheter positioned at the origin of the right carotid. The lesions at the left carotid artery were therefore exposed to the agents systemically. Infusion of AIM-PL at the 2 highest doses caused reduction of right carotid artery plaque area by the end a 90-minute infusion as assessed by IVUS analysis. Plaque area regression was confirmed by histology in carotid arteries receiving direct (500 and 1000 mg doses) and systemic (500 mg dose) delivery, 72 hours after the start of the treatment. Plaque lipid content was associated with significant and similar decreases in Oil Red O staining in both arteries. These results suggest AIM-PL complexes enhanced lipid removal from arteries is the mechanism responsible for the observed plaque changes.

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“…19 This is also the case of animals undergoing infusion of AIM-PL complexes, as assessed by histological examination of 3 animals euthanized without treatment (data not shown).…”

Section: Features Of Arterial Lesionsmentioning

confidence: 83%

Section: Experimental Protocolmentioning

confidence: 99%

Section: Ivus Imagingmentioning

confidence: 99%

“…compound (BDH Laboratory Supplies); sections were stained as described. 19 AIM detection was performed by using the same anti-human apoA-I antibody used for AIM plasma quantification.…”

Section: Histological Evaluation and Quantitation Of Lesionsmentioning

confidence: 99%

Section: Histological Evaluation and Quantitation Of Lesionsmentioning

confidence: 99%

See 3 more Smart Citations

Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Chiesa

Monteggia

Marchesi

et al. 2002

Circulation Research

195

104

View full text Add to dashboard Cite

show abstract

Contrast‐enhanced sonographic characteristics of neovascularization in carotid atherosclerotic plaques

Huang

Zou

et al. 2008

J of Clinical Ultrasound

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Dietary Supplements, Cholesterol and Cardiovascular Disease

Vasanthi

Kartal-Özer

Azzi

et al. 2010

Modern Dietary Fat Intakes in Disease Promotion

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Development of a lipid-rich, soft plaque in rabbits, monitored by histology and intravascular ultrasound

Cited by 37 publications

References 35 publications

Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Contrast‐enhanced sonographic characteristics of neovascularization in carotid atherosclerotic plaques

Dietary Supplements, Cholesterol and Cardiovascular Disease

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Development of a lipid-rich, soft plaque in rabbits, monitored by histology and intravascular ultrasound

Cited by 37 publications

References 35 publications

Recombinant Apolipoprotein A-I Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Recombinant Apolipoprotein A-I Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Contrast‐enhanced sonographic characteristics of neovascularization in carotid atherosclerotic plaques

Dietary Supplements, Cholesterol and Cardiovascular Disease

Contact Info

Product

Resources

About

Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks

Recombinant Apolipoprotein A-I _Milano Infusion Into Rabbit Carotid Artery Rapidly Removes Lipid From Fatty Streaks