Development of a light-initiated chemiluminescent assay for the quantitation of sIgE against egg white allergens based on component-resolved diagnosis

Bian, Ying; Liu, Changshan; She, Tiantian; Wang, Mengjuan; Yan, Jing; Wei, Dianjun; Li, Huiqiang

doi:10.1007/s00216-017-0791-y

Cited by 20 publications

(17 citation statements)

References 34 publications

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“…A bead-based light-initiated chemiluminescent assay (LICA) system was demonstrated in our previous studies to determine serum egg component-sIgE and sIgG4 profiles. LICA has been proven to have excellent analytical performances and a good correlation with Phadia Immu-noCAP tests in sIgE measurement [16,17]. Yet, only a limited profile of allergy tests has been introduced in our country; LICA could be a reliable complement in allergy screening and diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

Zhang

Shen

et al. 2020

BioMed Research International

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Background. Egg component-specific IgE can be useful to evaluate and diagnose egg allergy, but their prevalence and clinical significance remain unclear in the local population. Previous studies have led to contradictory results regarding the value of specific IgG and specific IgG4 in sensitization. Objective. We aimed to determine the level of specific IgE, IgG, and IgG4 antibodies to the major egg allergens in egg-allergic children. Methods. Children from 6 months to 10 years of age were recruited. Egg allergy was confirmed by either a strong clinical history or an increased egg white-sIgE level. Other allergies were diagnosed by reactivity to other allergens but without egg-related symptoms and history. The serum sIgE, sIgG, and sIgG4 levels to major egg allergenic components (Gal d 1, Gal d 2, Gal d 3, Gal d 4, and Gal d 5), sIgE level to egg white, and tIgE level were determined by light-initiated chemiluminescent assay (LICA), ELISA, or ImmunoCAP. Results. Egg-allergic children had significantly higher levels of sIgE, sIgG, and sIgG4 to egg components than nonallergic children. Gal d 2 was the predominant allergen, and Gal d 2 sIgE level correlated with the egg white-sIgE level. Ratios of sIgE/sIgG4 to egg components were highest before 1 year of age and dropped gradually in the first decade of life. Conclusion. Patterns of sIgE to egg components could distinguish different forms of egg allergy. Ratios of sIgE/sIgG4 could be useful in predicting tolerance in egg-sensitive subjects, but this needs further evaluation and investigation using more accurate models.

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Section: Discussionmentioning

confidence: 99%

“…Samples with sIgE to egg white ≥ 0:35 kU A /L were defined as positive. [16,17]. The results of sIgE and sIgG4 were calculated as relative light units (RLU).…”

Section: Determination Of Total Ige and Egg-specific Ige Levelsmentioning

confidence: 99%

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

Zhang

Shen

et al. 2020

BioMed Research International

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“…When the distance is within 200 nm, singlet oxygen will transfer from the donor beads to the acceptor beads under excitation, which will cause the acceptor beads to fluoresce at 520‐618 nm. This is a homogeneous method that is sensitive, specific, stable, and free of separation and washing steps and has high throughput . Because the concentration of E2 varies greatly in different periods, the detection method requires a large detection interval to meet the clinical needs.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of estradiol by competitive light‐initiated chemiluminescent assay using estriol as competitive antigen

Bian

et al. 2019

Clinical Laboratory Analysis

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Background Light‐initiated chemiluminescent assays (LICA) are homogeneous assays that are sensitive, specific, and free of separation and washing steps and have high throughput and high precision. Methods In this research, we developed a competitive method by LICA to achieve accurate quantification of estradiol (E2) in human serum. E2 competed with estriol (E3) for binding to anti‐human E2 antibodies. E3 was linked to biotin via bovine serum albumin as a linker. As this assay used competition between the labeled tracer and the analyte, an increase in E2 concentration will cause a signal decrease. Results The expected detection range of E2 was 20‐5000 pg/mL. The analytical and functional sensitivities were 7.16 and 13.7 pg/mL, respectively. The intra‐ and inter‐assay coefficients of variation were both below 15%, and the recovery rate ranged from 97.5% to 106.8%. The interference rates ranged from −3.6% to 5.4% and met detection requirements for E2 in hyperbilirubinemia, hemolysis, and lipemia in clinical samples. In addition, the cross‐reactivity rates between E2 and structural analogs and some reproductive hormones varied from 1.9% to 10.6% which showed that LICA is highly specific for E2. Moreover, our results showed high accordance with the IMMULITE 2000 (y = 0.6695x + 47.92, r2 = .843) and VIDAS systems (y = 1.099x − 821.5, r2 = .9392). Conclusion Our data show that the LICA, which is easy to automate, is a promising technique for quantification of E2 in human serum and could be used for clinical detection.

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“…The levels of sige and sigG 4 to rPen a 1 as well as its peptides were measured using a light-initiated chemiluminescent assay (lica) carried out as previously described (34,35). lica is based on the formation of nanoparticle pairs and luminescence oxygen channeling immunoassay technology (36,37).…”

Section: Preparation Of Biotinylated Recombinant Pen a 1 Ez-linkmentioning

confidence: 99%

“…lica is based on the formation of nanoparticle pairs and luminescence oxygen channeling immunoassay technology (36,37). Studies have shown that this new method possesses excellent performance characteristics with high sensitivity, broad analytical range and rapid turnaround cycles and is suitable for immunodetection of allergen sige and sigG 4 (34,35 well and incubated at 37˚C for 30 min with mild agitation. Subsequently, 150 µl streptavidin-coated sensibeads (Beyond Biotech, co., ltd.) were added.…”

Section: Preparation Of Biotinylated Recombinant Pen a 1 Ez-linkmentioning

confidence: 99%

IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China

Kong

et al. 2020

Mol Med Report

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Sensitization to allergens and their peptides varies among patients due to geographical or ethnic differences. The present study aimed to investigate immunoglobulin (ig)e and igG 4 responses to tropomyosin and its peptides in shrimp allergic patients from northern china. a total of 92 subjects were studied, including 35 shrimp allergic patients, 29 patients with house dust mite (HdM) and/or cockroach allergic patients and 28 healthy volunteers. Serum ige and igG 4 antibodies to recombinant shrimp tropomyosin (rPen a 1) and its peptides were measured by means of a light-initiated chemiluminescent assay. a total of 9 major sequential epitopes of Pen a 1 reported in the literature were synthesized. of 35 shrimp allergic patients, 25 (71.4%) had positive Pen 1-specific IgE (sige) antibodies and 22 (62.9%) contained measurable rPen a 1-specific igG 4 (sigG 4 ) antibodies. a strong igG 4 response accompanied the presence of ige to Pen a 1. none of the patients with HdM and/or cockroach allergy demonstrated ige reactivity to rPen a 1. The reaction frequency of ige binding epitope was 20-48%, while that of igG 4 binding epitope was 63.6-90.9%. The ige and igG 4 recognition patterns of the tropomyosin peptides demonstrated high interpatient heterogeneity. diversity of ige binding epitopes was positively correlated with Pen a 1 sige levels. in the study population, tropomyosin was a major allergen recognized by the majority of shrimp allergic patients, which is consistent with previous reports. However, none of the 9 epitopes are major (reaction frequency >50%) ige-binding regions, indicating the epitopes profile may be different in other regions.

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Development of a light-initiated chemiluminescent assay for the quantitation of sIgE against egg white allergens based on component-resolved diagnosis

Cited by 20 publications

References 34 publications

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

Quantitation of estradiol by competitive light‐initiated chemiluminescent assay using estriol as competitive antigen

IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China

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