Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk

Raysyan, Anna; Galvidis, Inna A.; Schneider, R.; Eremin, Sergei A.; Burkin, Maksim A.

doi:10.1016/j.jpba.2020.113450

Cited by 21 publications

(11 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Spotting conditions of the membrane were optimized. The influence of spotting parameters on the final characteristics of the assay has been reported by us previously [22]. In this specific case, optimization improved the limit of detection for BPA by a factor of roughly 100.…”

Section: Resultsmentioning

confidence: 54%

“…The preparation of the LMP-IgG conjugate was done using the protocol from our previous work [22]. Briefly, to 1 mL of 50 mM MES coupling buffer of pH 6.0, 100 µL of carboxy latex beads were added under stirring; subsequently, 24 µL of 200 mM EDC and 240 µL of 200 mM Sulfo-NHS in 1 mL activation buffer (50 mM MES, pH 6.0) were added [23].…”

Section: Antibody Immobilization On Latex Microparticlesmentioning

confidence: 99%

“…An XYZ piezoelectric non-contact spotter was used for this purpose [24]. The non-contact spotting protocol was published by us previously [22]. Briefly, to create the lines with optimum width of the testing zones of around 0.5-1.0 mm, several rows of spots spaced at a distance of 250 µ m from each other were applied.…”

Section: Assembly Of Lateral Flow Test Stripsmentioning

confidence: 99%

See 2 more Smart Citations

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Raysyan

Schneider

2021

Biosensors

Self Cite

View full text Add to dashboard Cite

One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods.

show abstract

Section: Resultsmentioning

confidence: 54%

Section: Antibody Immobilization On Latex Microparticlesmentioning

confidence: 99%

Section: Assembly Of Lateral Flow Test Stripsmentioning

confidence: 99%

See 1 more Smart Citation

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Raysyan

Schneider

2021

Biosensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…1) LFIA wurden vor Kurzem entwickelt, um eines unserer ersten Lebensmittel, die Muttermilch, im Selbsttest auf Rückstände von Makrolidantibiotika zu untersuchen. 9) Sensitivitätsoptimierte LFIA eignen sich auch dazu, Bisphenol A in Thermopapier (Kassenbons) und auf Tickets nachzuweisen (Abbildung 3). 10) Ein…”

Section: Sensoren Und Schnelltestsunclassified

Mit dem Testsystem zur Probe

Weller¹,

Schneider²,

Rurack³

2021

Nachr Chem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Enzyme-linked immunosorbent assay (ELISA) has been widely used for rapid detection of T&T in food samples [22][23][24]. Lateral flow immunoassay (LFIA) was developed to analyze six macrolide antibiotics in breast milk using latex particles labeled with BSA-clarithromycin antibody [25]. ELISA is used frequently for its high sensitivity, portability, and high throughput screening, but it was easily affected by matrix effects [26,27].…”

Section: Introductionmentioning

confidence: 99%

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Wang

Yang

et al. 2021

Foods

View full text Add to dashboard Cite

Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.

show abstract

Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk

Cited by 21 publications

References 22 publications

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

Mit dem Testsystem zur Probe

Development of Time-Resolved Fluorescence Immunochromatographic Assays for Simultaneously Detecting Tylosin and Tilmicosin in Milk in Group-Screening Manner

Contact Info

Product

Resources

About