Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection

Abstract

Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the detection of active C. psittaci infection was developed. The RAA-LF assay targeted the CPSIT_RS02830 gene of C. psittaci and could be accomplished in 15 min at a single temperature (39°C). The analytical sensitivity of the assay was as low as 1 × 100 copies/μl and no cross-reaction with some other intracellular pathogens was observed. Moreover, all feces samples from mice infected with C. psittaci at day-1 post-infection were positive in the RAA-LF assay. In conclusion, the RAA-LF assay provides a convenient, rapid, specific and sensitive method for detection of active C. psittaci infection and it is also suitable for C. psittaci detection in field.

Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay

“…In addition, combining the CRISPR system with RAA technology enables ultrasensitive detection of DNA or RNA single molecules ( Zhao et al., 2021 ; Duan et al., 2022 ; Zhang et al., 2022 ). Due to the characteristics of high sensitivity and specificity, low reaction time, simple operation, visual judgment of results, and suitability for on-site rapid diagnosis, RAA has been widely applied in the detection of various pathogenic microorganisms ( Tu et al., 2021 ; Jiao et al., 2022 ; Wu et al., 2022 ).…”