Development of a lateral flow immunoassay for rapid quantitation of carbendazim in agricultural products

Deng, Huimin; Cai, Xiwen; Ji, Yuan; Ding, Yan; Yang, Fei; Liu, Shanshan; Deji, Zhuoma; Wang, Ying; Bian, Zhaoyang; Tang, Gangling; Fan, Ziyan; Huang, Zhenzhen

doi:10.1016/j.microc.2022.107495

Cited by 11 publications

(3 citation statements)

References 31 publications

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“…Currently, lateral flow immunoassays (LFIAs) have played a significant role during the COVID-19 epidemic . They can be used for COVID-19 infection screening and also be used for antibody detection because they are easy to use and affordable. , Recently, LFIAs have been demonstrated for detecting NAbs-COVID-19 and can precisely quantify NAbs-COVID-19 levels in clinical serum samples. − The detection principle relies on the use of immobilized ACE2 on the T-line, which binds with the free RBD from NAbs–RBD complex . However, this sensor cannot indicate the efficacy of the NAb-COVID-19 against multiple strains due to its use of a single RBD.…”

Section: Introductionmentioning

confidence: 99%

“… 20 They can be used for COVID-19 infection screening and also be used for antibody detection because they are easy to use and affordable. 21 , 22 Recently, LFIAs have been demonstrated for detecting NAbs-COVID-19 and can precisely quantify NAbs-COVID-19 levels in clinical serum samples. 23 − 25 The detection principle relies on the use of immobilized ACE2 on the T-line, which binds with the free RBD from NAbs–RBD complex.…”

Section: Introductionmentioning

confidence: 99%

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Overlaid Lateral Flow Immunoassay for the Simultaneous Detection of Two Variant-Specific SARS-CoV-2 Neutralizing Antibodies

Deenin,

Khongchareonporn,

Ruxrungtham

et al. 2024

Anal. Chem.

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vaccines have been provided to the general public to build immunity since the 2019 coronavirus pandemic. Once vaccinated, SARS-CoV-2 neutralizing antibodies (NAbs-COVID-19) are needed for excellent protection against COVID-19. However, monitoring NAbs-COVID-19 is complicated and requires hospital visits. Moreover, the resulting NAbs-COVID-19 are effective against different strains of COVID-19 depending on the type of vaccine received. Here, an overlaid lateral flow immunoassay (O-LFIA) was developed for the simultaneous detection of two NAbs-COVID-19 against different virus strains, Delta and Omicron. The O-LFIA was visualized with two T-lines with a single device using competition between the free antigen and the antigen-binding antibody. Angiotensin-converting enzyme 2 (ACE2) immobilized on the T-line binds to the antigen remaining after antibody binding. Under the optimum conditions, the proposed device exhibited 50% inhibition concentrations (IC 50 values) of 45.1 and 53.6 ng/mL for the Delta and Omicron variants, respectively. Additionally, the proposed platform was applied to realworld samples of animal and human serum, and the developed immunoassay provided results that were in good agreement with those obtained with the standard method. In conclusion, this developed O-LFIA can be used as an alternative method to detect NAbs-COVID-19 and can be enabled for future advancements toward commercialization.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overlaid Lateral Flow Immunoassay for the Simultaneous Detection of Two Variant-Specific SARS-CoV-2 Neutralizing Antibodies

Deenin,

Khongchareonporn,

Ruxrungtham

et al. 2024

Anal. Chem.

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“…Immunochromatographic test strips commonly employ a recognition probe of specific antibodies and a signaling probe of diverse tags, for instance, colloidal gold nanoparticles (CGNs), 13 fluorescent nanobeads, 14 quantum dots, 15 and up-conversion nanoparticles. 16 These test strips have displayed outstanding performances including simplicity, rapidity, portability, long shelf-time, good stability, low cost, and remarkable user-friendliness.…”

Section: Introductionmentioning

confidence: 99%

Development of a colloidal gold immunochromatographic test strip for the rapid detection of iprodione

Deng

Chen

et al. 2022

Anal. Methods

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Integration of a biocompatible metal-phenolic network and fluorescence microspheres as labels for sensitive and stable detection of carbendazim with a lateral flow immunoassay

Chen,

Tang,

Guo

et al. 2024

Food Chemistry

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Development of a lateral flow immunoassay for rapid quantitation of carbendazim in agricultural products

Cited by 11 publications

References 31 publications

Overlaid Lateral Flow Immunoassay for the Simultaneous Detection of Two Variant-Specific SARS-CoV-2 Neutralizing Antibodies

Overlaid Lateral Flow Immunoassay for the Simultaneous Detection of Two Variant-Specific SARS-CoV-2 Neutralizing Antibodies

Development of a colloidal gold immunochromatographic test strip for the rapid detection of iprodione

Integration of a biocompatible metal-phenolic network and fluorescence microspheres as labels for sensitive and stable detection of carbendazim with a lateral flow immunoassay

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