Development of a laser plasma x-ray microscope to observe
live hydrated biological specimens

Kado, M.; Daido, H.; Yamamoto, Yoshimasa; Shinohara, Kunio; Richardson, Martin

doi:10.1002/lapl.200510074

Cited by 11 publications

(5 citation statements)

References 5 publications

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“…The visualization of dry or hydrated human macrophages and Leydig cells demonstrated that SXCM allows the morphologies of actual intact cells to be viewed and encourages further technological development for biomedical research [45,51,[60][61][62]. This technique also offered the possibility of monitoring the morphological changes in microorganisms (e.g., upon exposure to antibiotics), as was demonstrated for the Gram-negative bacterium, Pseudomonas aeruginosa, and Candida albicans, a yeast, among others [63,64].…”

Section: Cell Imaging With Sxcmmentioning

confidence: 78%

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

et al. 2022

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Soft X-ray microscopy is a powerful technique for imaging cells with nanometer resolution in their native state without chemical fixation, staining, or sectioning. The studies performed in several laboratories have demonstrated the potential of applying this technique for imaging the internal structures of intact cells. However, it is currently used mainly on synchrotrons with restricted access. Moreover, the operation of these instruments and the associated sample-preparation protocols require interdisciplinary and highly specialized personnel, limiting their wide application in practice. This is why soft X-ray microscopy is not commonly used in biological laboratories as an imaging tool. Thus, a laboratory-based and user-friendly soft X-ray contact microscope would facilitate the work of biologists. A compact, desk-top laboratory setup for soft X-ray contact microscopy (SXCM) based on a laser-plasma soft X-ray source, which can be used in any biological laboratory, together with several applications for biological imaging, are described. Moreover, the perspectives of the correlation of SXCM with other super-resolution imaging techniques based on the current literature are discussed.

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Section: Cell Imaging With Sxcmmentioning

confidence: 78%

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

et al. 2022

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“…Specimens used in this work were macrophages (MH-S mouse alveolar macrophage cell line), because of their well known morphological features 7,8 . About 1 μl of the cell suspension in culture medium was directly dropped onto the PMMA.…”

Section: Resultsmentioning

confidence: 99%

Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells

et al. 2011

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Ultra thin gold films having a thickness of 20-30 nm are favorable laser plasma targets for a soft x-ray microscopy, because the ultra thin films emit intense soft x-rays at the wavelength of water window region from the rear side with respect to the surface irradiated with short pulse laser. Using rear side emissions, the distance between the x-ray source and the specimens can be reduced so that the x-ray flux on specimens increases. In addition, the microscope system can be designed to be compact when the specimen holder and x-ray source are combined in one piece. In the present study, the biological specimen holder combined with a gold ultra thin film plasma target has been developed for a contact-type soft x-ray microscope. This x-ray microscope system needs not any x-ray optics such as a condenser and/or an objective optics which causes a decrease in x-ray photons for imaging. Specimen holder equipped with the plasma target keeps biological specimens at wet condition in vacuum. In this study, x-ray images of hydrated living cells (MH-S mouse alveolar macrophage cell line) have been obtained successfully by use of the newly developed specimen holder. These experimental results reveal that the soft x-ray image can be taken safely. Specimen holder combined with plasma x-ray source will be a key component of a compact soft x-ray microscope using in a laboratory.

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“…The duration time of emitted soft x-rays from laser produced plasmas depends on that of the irradiated laser pulses which are generally nano-second or pico-second. This short duration time of laser produced plasmas results in short exposure time to specimens and favorable time to take a snap shot of moving cells with a high spatial resolution 3,4 . In addition, the short exposure time will avoid the effects of radiation damage on the images of living cells.…”

Section: Introductionmentioning

confidence: 99%

Observations of the intense soft x-ray emissions from ultrathin Au films irradiated with high contrast laser pulses

et al. 2010

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Observation of soft x-ray emissions from laser produced plasmas using ultra thin film targets has been carried out. Au ultra thin films deposited on silicon nitride membranes were irradiated with a high contrast Nd:glass laser pulses. The spectral properties of emitted soft x-rays were monitored with an x-ray spectrograph from the membrane side. The observed emission intensities had a clear dependence on the Au film thickness. The results suggest that most of the laser energy irradiated is absorbed by the Au films and few of the energy goes into the silicon nitride membranes, which means an efficient laser energy deposition to the ultra thin Au film target and a high energy conversion rate from laser to x-rays.

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Development of a laser plasma x-ray microscope to observe live hydrated biological specimens

Abstract: Investigating the structure and the function of life ob-

Cited by 11 publications

References 5 publications

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

Nanometer-Resolution Imaging of Living Cells Using Soft X-ray Contact Microscopy

Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells

Observations of the intense soft x-ray emissions from ultrathin Au films irradiated with high contrast laser pulses

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