Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker

Chen, Ching-Sung; Chang, Ku-Ning; Chen, Yinghua; Lee, Chih‐Kung; Lee, Bryan Yong-Jay; Lee, Adam Shih‐Yuan

doi:10.1016/j.bios.2010.11.051

Cited by 23 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most of the previous studies used an external reference electrode and a counter electrode for EIS-based immunosensors which is not conducive to the development of portable and miniaturized immunosensors. 10,12,13 In this study, the Pt-deposited ORE 1st and IRE 1st electrodes were used not only to drive the ACEK vortex but also, respectively, as the reference electrode and the counter electrode for EIS measurement. Wu's study showed that the Pd-deposited electrode provides a stable equilibrium potential of Fe(CN) 6 3À/4À for EIS measurement following the modification of thiolated molecules.…”

Section: Eis-based Sensormentioning

confidence: 99%

“…Immunoassay relies on the use of specific antibodies to detect antigenic targets for various applications, 1,2 including clinical diagnostics, 3 food safety, 4 and the detection of environmental pollutants. 5 Label-free techniques, such as surface plasmon resonance, 6 quartz crystal microbalance, 7 mass-sensitive microcantilever, 8 and electrochemical impedance spectroscopy (EIS)based immunosensors, [9][10][11] have attracted increasing attention due to the reduced number of immunoassay steps, improved time efficiency, and reduced reagent cost. In particular, the EISbased immunosensor presents high sensitivity, high cost-effectiveness, and ease of mass production and miniaturization.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the EISbased immunosensor presents high sensitivity, high cost-effectiveness, and ease of mass production and miniaturization. 10,12,13 In general, immunoreactions require tens of minutes to reach the hybridization plateau in a stationary solution, 14 thus limiting its applicability for in vitro diagnostics. Moreover, the lower the analyte concentration in a stationary solution, the longer the immunosensor's saturated immunoreaction due to the lower diffusive flux from bulk solution to the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An impedimetric bioaffinity sensing chip integrated with the long-range DC-biased AC electrokinetic centripetal vortex produced in a high conductivity solution

Lin

Liu

2018

Biomicrofluidics

View full text Add to dashboard Cite

Immunoreaction of specific antibodies to antigens is widely used in numerous immunoanalysis applications. However, diffusion-dominated transport in stationary solutions limits the rate and binding density of immunoreaction. This research describes the construction of chip-type concentric multi-double ring electrodes and single central disk electrode. A +1 V-biased 6 V voltage was applied to the multi-double ring electrodes to induce a long-range DC-biased AC electrokinetic flow (ACEKF). The immunoreaction was quantified by electrochemical impedance spectrum (EIS). Fluorescence-labeled secondary antibody (FLSA) and protein A were exemplified as an immunoreacting model to demonstrate the effect of ACEKF on immunoreaction efficiency. The results showed that FLSA binding can reach a plateau in 8 min with the DC-biased ACEKF vortex, and the increment of electron transfer resistance is 2.26 times larger than that obtained in the unstirred solution. The sensitivity of the calibration curves obtained by EIS detection with the aid of DC-biased ACEKF vortex is 1.51 times larger than that obtained in an unstirred solution. The label-free EIS-based sensing chip integrated with the long-range DC-biased ACEKF vortex promises to facilitate immunoreaction efficiency, which is beneficial for the development of a miniature and fast-detection diagnostic device.

show abstract

Section: Eis-based Sensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An impedimetric bioaffinity sensing chip integrated with the long-range DC-biased AC electrokinetic centripetal vortex produced in a high conductivity solution

Lin

Liu

2018

Biomicrofluidics

View full text Add to dashboard Cite

show abstract

“…The degree of electrode membrane activation by enzyme can be estimated from the electrode signal in the presence of substrate. There have been, however, limited studies on this type of immunosensor coupled with capacitance or impedance measurement, [8][9][10] which may be due to difficulties in linking an enzyme reaction to a method for changing the impedance or capacitance of an electrode. McNeil et al introduced ability of sensor based on the measurement of impedance change by using polymer coatings on electrodes and analysis the antigen-antibody reaction qualitatively by immunostrips and quantitatively by EIS.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG

Yi¹,

Choi²,

Kim³

et al. 2011

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

This study describes the development of an electrochemical array immunochip for the detection of IgG. Interdigitated immunochip platforms were fabricated by sputtering gold on a glass wafer by using MEMS process and then were coated with Eudragit S100, an enteric polymer, forming an insulating layer over the working area of immunochips. The breakdown of the polymer layer was exemplified by the catalytic action of urease which, in the presence of urea, caused an alkaline pH change. This subsequently caused an increase of the double layer capacitance of the underlying electrode. Used in conjunction with a competitive immunoassay format, this allowed the ratio of initial to final electrode capacitance to be directly linked with the concentration of analyte, i.e. IgG. Responses to IgG could be detected at IgG concentration as low as 250 ngmL −1 and showed good linearity up to IgG concentration as high as 20 µgmL .

show abstract

“…43 Karolina Dziabowska e colaboradores também menciona que a voltametria cíclica e a impedância eletroquímica, são promissoras no desenvolvimento de sistemas completos de detecção automática, sem necessidade de pré-tratamento de amostra, análise rápida e interfaces com dispositivos de telecomunicação (smartphones e tablets). 44 Recentemente, grupos de pesquisas na área de biossensores têm desenvolvido diferentes plataformas de sensores, com o uso dessas técnicas para detectar reações de antígenoanticorpo, 45 de hibridação de DNA, 46 reações enzimáticas 47 e interação receptor-ligante. 48 Baseado nas vantagens supramencionadas, optou-se utilizar no presente projeto de dissertação as técnicas de voltametria cíclica e a espectroscopia de impedância eletroquímica.…”

unclassified

Desenvolvimento de um sistema biossensor baseado em filmes >i<layer-by-layer>/i< de PAH e ácido fólico para detecção de células cancerosas com receptores de folato

Correia¹

View full text Add to dashboard Cite

Em primeiro lugar eu agradeço a Deus pela força e graça durante esta caminhada.Aos meus pais Francisco Lima e Francisca de Lourdes pelo apoio, incentivo, amor, força e educação que me deram durante toda a vida.Aos meus irmãos Alysson e Aliene.Ao meu noivo Oziel, pelo amor, companheirismo e compreensão em cada momento difícil.Agradeço por acreditar em mim, ouvir e apoiar os meus sonhos, em você encontrei alívio e paz durante as frustações e angustias vividas, também grata por se alegrar comigo nos momentos de sucesso e realização. À toda a minha família pelo incentivo e apoio.Ao Prof. Dr. Valtencir Zucolotto, pela grande e rica oportunidade de integrar ao seu grupo de pesquisa, pela sua orientação durante o mestrado, a qual muito me ensinou, contribuindo para meu crescimento científico e intelectual.Ao Dr. Nirton Cristi Silva Vieira pelas ajuda e apoio na realização deste mestrado. À minha grande amiga Andressa, que acompanhou de perto os últimos momentos do meu mestrado, compartilhando alegrias e dificuldades.A todos os colegas e funcionários do GNano, pela amizade e apoio profissional.A todos os funcionários do Instituto de Física de São Carlos.Ao CNPQ, pela concessão da bolsa de mestrado e pelo apoio financeiro para a realização desta pesquisa. "Ó profundidade das riquezas, tanto da sabedoria, como da ciência de Deus! Quão insondáveis são os seus juízos, e quão inescrutáveis os seus caminhos! Por que quem compreendeu a mente do Senhor? Ou quem foi seu conselheiro? Ou quem lhe deu primeiro a ele, para que lhe seja recompensado? Porque Dele e por Ele, e para Ele, são todas as coisas, glória, pois, a Ele eternamente. Amém" Romanos 11:33-36 ABSTRACT CORREIA, A. R. Development of a biossensor system based on layer-by-layer films of PAH and folic acid for detection of cancer cells with folate receptors. 2019. 98 p. Dissertação (Mestrado em Ciências) -Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, 2019.Early detection of tumor cells plays an important role in the effective treatment of cancer.Some conventional methods, such as imaging and radiolabels, are effective, but complex and costly. On the other hand, electrochemical biosensors have attracted considerable attention due to its advantages over conventional techniques, such as high sensitivity, simplicity, fast response, miniaturization and low cost. Recent studies have shown that tumor cell surfaces, especially in gynecologic cancers, express more folate receptors than normal cells. Folate receptors are folate binding proteins which have a high affinity for folic acid (FA) (K d < 1 nmol L -1 ). In this study a biosensor was developed for the detection of tumor cells HeLa (cervical cancer cells), based on electrochemical platform modified with folic acid. HMEC (human mammary epithelial cells) was used as the negative control and a selectivity test was carried out with polymer Poly (acrylic acid) -PAA. The surface of the biosensor was modified with self-assembly layer-by-layer (LbL) films containing PAH (polyamine hydrochloride) and folic...

show abstract

Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker

Cited by 23 publications

References 42 publications

An impedimetric bioaffinity sensing chip integrated with the long-range DC-biased AC electrokinetic centripetal vortex produced in a high conductivity solution

An impedimetric bioaffinity sensing chip integrated with the long-range DC-biased AC electrokinetic centripetal vortex produced in a high conductivity solution

An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG

Desenvolvimento de um sistema biossensor baseado em filmes >i<layer-by-layer>/i< de PAH e ácido fólico para detecção de células cancerosas com receptores de folato

Contact Info

Product

Resources

About