Development of a label free electrochemical sensor based on a sensitive monoclonal antibody for the detection of tiamulin

Wang, Aiping; Xiaojuan, You; Liu, Hongliang; Zhou, Jingming; Chen, Yumei; Zhang, Chenyang; Ma, Kaikai; Liu, Yankai; Ding, Peiyang; Qi, Yanhua; Zhang, Gaiping

doi:10.1016/j.foodchem.2021.130573

Cited by 12 publications

(6 citation statements)

References 36 publications

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“…Electrochemical biosensors offer a green, rapid, highly sensitive, and cost-effective approach for detecting small molecules, 86 , 87 , 88 , 89 proteins, 90 nucleic acids, 83 and pathogens. 91 Leveraging the ultra-sensitive nature of electrochemical biosensors, OBServe holds the potential to eliminate the requirement for expensive and time-consuming preamplification process.…”

Section: Discussionmentioning

confidence: 99%

On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay

Wang,

Zhang

et al. 2024

iScience

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Section: Discussionmentioning

confidence: 99%

On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay

Wang,

Zhang

et al. 2024

iScience

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“…The rGO-PEI-Ag nanocomposites were prepared according to the literature with a little change [ 36 , 37 ]. Figure 1 showed the basic synthesis process of nanomaterials.…”

Section: Methodsmentioning

confidence: 99%

An Electrochemical Immunosensor Based on SPA and rGO-PEI-Ag-Nf for the Detection of Arsanilic Acid

Wang

Zhang

et al. 2021

Molecules

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A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation of nanomaterials, polyethyleneimine (PEI) improved the stability of the solution and acted as a reducing agent to generate reduced graphene oxide (rGO) with relatively strong conductivity, thereby promoting the transfer of electrons. The dual conductivity of rGO and silver nanoparticles (AgNPs) improved the sensitivity of the sensor. The synthesis of nanomaterials were confirmed by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy and scanning electron microscopy. In the optimal experiment conditions, the sensor could achieve the detection range of 0.50–500 ng mL−1 and the limit of detection (LOD) of 0.38 ng mL−1 (S/N = 3). Moreover, the sensor exhibited excellent specificity and acceptable stability, suggesting that the proposed sensor possessed a good potential in ASA detection. Thus, the as-prepared biosensor may be a potential way for detecting other antibiotics in meat and animal-derived foods.

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“…The most prolific adducts and other interrupting species were identified using ultrahigh-resolution FTICR-MS observations, which offered fully resolved isotopic distributions of intact mAb enabling the detection of the most numerous adducts and other interfering species [ 174 ]. Wang et al [ 196 ] created a label-free electrochemical sensor from murine mAb utilizing AuNPs (gold nanoparticles) and Ag-GO (silver-graphene oxide) nanocomposites to detect tiamulin (TML), which is a pleuromutilin antibiotic used in veterinary medicine for pigs and poultry. A broad detection range of TML between 0.01–1000 ng mL −1 was exhibited.…”

Section: Analytical Techniques For Mabs Analysesmentioning

confidence: 99%

Analytical Techniques for the Characterization and Quantification of Monoclonal Antibodies

Alhazmi

Albratty

2023

Pharmaceuticals

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Monoclonal antibodies (mAbs) are a fast-growing class of biopharmaceuticals. They are widely used in the identification and detection of cell makers, serum analytes, and pathogenic agents, and are remarkably used for the cure of autoimmune diseases, infectious diseases, or malignancies. The successful application of therapeutic mAbs is based on their ability to precisely interact with their appropriate target sites. The precision of mAbs rely on the isolation techniques delivering pure, consistent, stable, and safe lots that can be used for analytical, diagnostic, or therapeutic applications. During the creation of a biologic, the key quality features of a particular mAb, such as structure, post-translational modifications, and activities at the biomolecular and cellular levels, must be characterized and profiled in great detail. This implies the requirement of powerful state of the art analytical techniques for quality control and characterization of mAbs. Until now, various analytical techniques have been developed to characterize and quantify the mAbs according to the regulatory guidelines. The present review summarizes the major techniques used for the analyses of mAbs which include chromatographic, electrophoretic, spectroscopic, and electrochemical methods in addition to the modifications in these methods for improving the quality of mAbs. This compilation of major analytical techniques will help students and researchers to have an overview of the methodologies employed by the biopharmaceutical industry for structural characterization of mAbs for eventual release of therapeutics in the drug market.

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Development of a label free electrochemical sensor based on a sensitive monoclonal antibody for the detection of tiamulin

Cited by 12 publications

References 36 publications

On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay

On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay

An Electrochemical Immunosensor Based on SPA and rGO-PEI-Ag-Nf for the Detection of Arsanilic Acid

Analytical Techniques for the Characterization and Quantification of Monoclonal Antibodies

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