Development of a HPLC‐MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats

Abstract

A sensitive and reliable HPLC-MS/MS method has been developed and validated for simultaneous determination of eleven bioactive compounds (rhein, emodin, stilbene glycoside, liquiritin, ononin, verbascoside, gallic acid, schisandrin, liquiritigenin, glycyrrhizic acid, and isoliquiritigenin) in rat plasma after oral administration of Tongmai Yangxin Pill. The collected plasma samples were prepared by liquid-liquid extraction with ethyl acetate after acidification. Eleven compounds were separated on a CORTECS™ C18 column with mobile phases consisting of 0.1% formic acid in deionized water and acetonitrile. The flow rate was 0.3 mL/min. The detection was performed on a tandem mass system with an electrospray ionization (ESI) source in both positive and negative ionization using multiple-reaction monitoring (MRM) mode. The calibration curves were linear over the range of 8-2000 ng/mL for glycyrrhizic acid; 4-1000 ng/mL for liquiritin; 0.8-200 ng/mL for emodin, gallic acid, ononin, schisandrin, and stilbene glycoside; 0.4-100 ng/mL for isoliquiritigenin, liquiritigenin, rhein, and verbascoside, respectively. The intra- and interday precision of the analytes were less than 9.3% and 8.5%. The intra- and interday accuracy were in the range of -14.0% to 10.3% and -6.5% to 9.6%. Meanwhile, the extraction recovery of the analytes in plasma samples ranged from 85.2% to 109.1% and matrix effect from 89.2% to 113.4%. The developed method was successfully applied to the pharmacokinetics of eleven bioactive compounds in rat plasma after oral administration of Tongmai Yangxin Pill prescription.

UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of Cyperus conglomeratus Rottb. Alcoholic Extract

“…Peak 50 exhibited a parent ion at m / z 283.0275 (C 15 H 8 O 6 ), displaying successive losses of 28 and 44 amu ( Figure S7 ) and annotated as trihydroxycoumestan in accordance with published data [ 39 ]. Likewise, peak 53 exhibited [M − H] − at m / z 485.1269, C 28 H 22 O 8 − ( Figure S8 ), and identified as longusol C, a stilbene dimer previously isolated from C. longus [ 31 ].…”

Tongmai Yangxin intervening in myocardial remodeling after PCI for coronary heart disease: study protocol for a double-blind, randomized controlled trial

“…TMYX, an effective patent medicine that is commonly used to treat CHD, arrhythmias, and HF, has bright clinical application prospects. With regard to the different pathophysiological factors of cardiac remodeling, previous studies of TMYX have indicated that TMYX can inhibit cardiac hypertrophy; decrease the apoptosis rate of myocardial cells; decrease anti-inflammatory, antioxidant, antiarrhythmic, and myocardial protection; promote angiogenesis; and ameliorate cardiac energy metabolism in a rat model of MI and ischemic reperfusion injury [12][13][14]. Currently, cardiac ultrasound (CUS) [5,15,16] and CMRI [5,17] are the major tools for the evaluation of ventricular remodeling and myocardial fibrosis at the international level.…”

Tongmai Yangxin Pills Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

“…Previous studies have shown that SIRT3 regulates mitochondrial biosynthesis by activating PGC-1α. Studies (12) have shown that myocardial protective active substances in TMYXP, the identi ed compounds are mainly derived from licorice, Schisandra chinensis, Ophiopogon. Glycyrrhizin, the main active compound of TMYXP, can activate the AMPK/SIRT1/PGC-1α signaling pathway in the kidneys of db/db mice with diabetic nephropathy in rats, and play a role in inhibiting ROS activation and apoptosis (14).…”

“…Tongmai Yangxin Pill (TMYXP) is a traditional Chinese medicine composed of 11 Chinese herbal medicines, including Radix Scutellariae, Glycyrrhiza uralensis, Radix Glycyrrhizae, Schisandra chinensis, Radix Polygonum multi orum, etc. It has the effect of nourishing the heart and nourishing the blood, tongmai analgesia, and is used for the treatment of chest pain, heartache, angina pectoris, arrhythmia, etc (12). Studies (13) have shown that the myocardial protective effect of TMYXP may be related to antioxidant effects, but its mechanism has not yet been elucidated.…”