Development of a HPLC–MS/MS method for the simultaneous determination of nifedipine and lidocaine in human plasma

Grigoriev, A. V.; Nikitina, Alexandra; Yaroshenko, Irina; Sidorova, A. A.

doi:10.1016/j.jpba.2016.08.004

Cited by 26 publications

(16 citation statements)

References 18 publications

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“…Many studies have reported HPLC, LC, and GC-MS methods for determination of lidocaine [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. However, only few of them have described bioanalysis method for its metabolites.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Lidocaine and Metabolite Pharmacokinetics in Hyaluronic Acid Injection

et al. 2021

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Lidocaine-incorporated hyaluronic acid injection (LHA) is considered a promising way to increase patient compliance. Various reviews and analyses have been conducted to verify that the addition of lidocaine had no effect on the product quality of hyaluronic acid injections. However, possible pharmacokinetic (PK) alterations of lidocaine and its active metabolites, monoethylglycylxylidide (MEGX) and glycylxylidide (GX), in hyaluronic acid injection have not been studied so far. Thus, the objective of this study was to evaluate lidocaine and its metabolite PK after 0.3% lidocaine solution or LHA injection and to investigate any changes in PK profiles of lidocaine and its active metabolites. To do this, a novel bio-analytical method for simultaneous determination of lidocaine, MEGX, and GX in rat plasma was developed and validated. Then, plasma concentrations of lidocaine and its active metabolites MEGX and GX following subcutaneous (SC) injection of 0.3% lidocaine solution or LHA with 0.3–1% lidocaine in male Sprague-Dawley rats were successfully determined. The obtained data were used to develop a parent-metabolite pharmacokinetic (PK) model for LHA injection. The half-life, dose-normalized Cmax, and AUCinf of lidocaine after SC injection of lidocaine solution and LHA did not show statistically significant difference. The PK characteristics of lidocaine after LHA administration were best captured using a two-compartment model with combined first-order and transit absorption and its clearance described with Michaelis–Menten and first-order elimination kinetics. Two one-compartment models were consecutively added to the parent model for the metabolites. In conclusion, the incorporation of lidocaine in hyaluronic acid filler injection did not alter the chemical’s pharmacokinetic characteristics.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Lidocaine and Metabolite Pharmacokinetics in Hyaluronic Acid Injection

et al. 2021

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“…Lidocaine has good activity when it is applied to superficial body sites, penetrating mucous membranes to reduce the sensation of pain. 2 Additionally, lidocaine is also an antiarrhythmic drug, which is administered intravenously for the prevention and treatment of ventricular arrhythmias in patients with acute myocardial infarction or other cardiac diseases. 3 Nowadays, lidocaine is prevailing used in cosmetic plastic surgery under local infiltration anesthesia.…”

Section: Introductionmentioning

confidence: 99%

Functional assessment of <em>CYP3A4</em> allelic variants on lidocaine metabolism in vitro

Fang¹,

Tang²,

Xu³

et al. 2017

DDDT

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AimHuman cytochrome P450 3A4 is the most abundant isoform of P450 enzyme in the liver. It plays an important role in the metabolism of wide variety of xenobiotic and endogenous substrates. So far, there are few reports about the functional characterization of CYP3A4 variants in terms of specific substrates. The aim of this study was to systematically investigate the genetic polymorphisms of 23 CYP3A4 alleles and evaluate their catalytic activities on the metabolism of lidocaine in vitro.Methods and resultsThe wild-type and 22 CYP3A4 variants were expressed in Spodoptera frugiperda 21 insect cells. Then the insect microsomes were incubated with the CYP3A4-specific substrate lidocaine. Reactions were performed with 50–3,000 µM for 60 min at 37°C. Lidocaine and its metabolite monoethylglycinexylidide were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry system. Of the 23 CYP3A4 allelic variants tested, 2 variants (CYP3A4*17 and CYP3A4*30) had no detectable enzyme activity; and 5 variants (CYP3A4*2, CYP3A4*5, CYP3A4*9, CYP3A4*16 and CYP3A4*24) showed significantly decreased intrinsic clearance values compared with wild-type CYP3A4*1.ConclusionAs the first study of all these CYP3A4 alleles for lidocaine metabolism, our results in vitro assessment may provide novel insights into the allele-specific and substrate-specific activity of CYP3A4 and may also offer a reference to the personalized treatment of lidocaine in a clinical setting.

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“…Only 2 μl of resulting sample was injected into the HPLC system, which helped to extend the life of the chromatographic column. The current method, as well as the earlier ones (Grigoriev, Nikitina, Yaroshenko, & Sidorova, 2016), was validated and successfully applied to a clinical trial.…”

Section: Introductionmentioning

confidence: 99%

4‐Methylamino antipyrine determination in human plasma by high‐performance liquid chromatography tandem mass spectrometry

Nikitina¹,

Gildeeva

Grigoriev

et al. 2020

Biomedical Chromatography

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In the present study, a simple and rapid method for metamizole metabolite 4‐methylamino antipyrine (MAA) determination in human plasma was developed, validated and successfully applied to a clinical trial. Chromatographic separation was achieved in HILIC mode on a YMC‐Pack SIL column (100 × 2.0 mm; S‐5 μm, 30 nm), with a mobile phase consisting of acetonitrile, water and formic acid. Protein precipitation of a small plasma volume using acetonitrile was selected for sample preparation. The multiple reaction monitoring transitions in the positive ionization mode were m/z 218.2 → 56.2 for MAA and m/z 221.2 → 56.2 for MAA‐d3 (IS, internal standard). Concentration levels of MAA calibration standards were in the range of 0.100–20 μg/ml. Metamizole conversion into MAA in both water and organic media was investigated, and the level of the conversion in commercially available injection solutions was estimated.

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Development of a HPLC–MS/MS method for the simultaneous determination of nifedipine and lidocaine in human plasma

Cited by 26 publications

References 18 publications

Evaluation of Lidocaine and Metabolite Pharmacokinetics in Hyaluronic Acid Injection

Evaluation of Lidocaine and Metabolite Pharmacokinetics in Hyaluronic Acid Injection

Functional assessment of <em>CYP3A4</em> allelic variants on lidocaine metabolism in vitro

4‐Methylamino antipyrine determination in human plasma by high‐performance liquid chromatography tandem mass spectrometry

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