Development of a Homogeneous Assay to Measure Remnant Lipoprotein Cholesterol

Miyauchi, Kazuhito; Kayahara, Norihiko; Ishigami, Masato; Kuwata, Hideyuki; Mori, H; Sugiuchi, Hiroyuki; Irie, Tetsumi; Tanaka, Akira; Yamashita, Shizuya; Yamamura, Taku

doi:10.1373/clinchem.2007.092296

Cited by 51 publications

(47 citation statements)

References 23 publications

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“…Furthermore, the increased absorption of cholesterol and related phenomenon have been described in some populations at an elevated risk for atherosclerosis, such as those with obesity 18) , type 2 diabetes mellitus (T2DM) 19) and those treated with hemodialysis 20) . These previous studies raise the hypothesis that developed by Miyauchi et al 21) with commercial reagents (MetaboLead RemL-C test, Kyowa Medex Co., Ltd., Tokyo, Japan). The serum campesterol and lathosterol levels were measured as markers of intestinal cholesterol absorption and hepatic cholesterol synthesis, respectively, using gas chromatography at SRL,…”

Section: Subjectsmentioning

confidence: 64%

Kidney Function, Cholesterol Absorption and Remnant Lipoprotein Accumulation in Patients with Diabetes Mellitus

Sonoda

Shoji

Kimoto³

et al. 2014

JAT

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Aim: Remnant lipoproteins are atherogenic and increased in patients with type 2 diabetes mellitus (T2DM), chronic kidney disease (CKD) and other conditions. Thus far, information is limited regarding the synthesis and absorption of cholesterol in CKD patients and a possible link to the remnant levels. We examined possible alterations in serum markers of cholesterol synthesis and absorption and their potential associations with remnant lipoproteins in patients with CKD. Methods: The subjects included 146 consecutive patients with T2DM in various stages of CKD. We measured the levels of remnant lipoprotein cholesterol (RemL-C), lathosterol (a cholesterol synthesis marker) and campesterol (a cholesterol absorption marker). The urinary albumin to creatinine ratio (U-ACR) and estimated glomerular filtration rate (eGFR) were used to describe the degree of CKD. Results: The median (interquartile range) levels of RemL-C, lathosterol and campesterol were 14.5 (11.5-23.4) mg/dL, 2.1 (1.7-2.9) μg/mL and 2.3 (1.7-3.0) μg/mL, respectively. The RemL-C level was positively correlated with the U-ACR and inversely correlated with the eGFR. The RemL-C level was positively correlated with both the lathosterol and campesterol levels. The lathosterol level was not significantly correlated with the U-ACR, although it was positively correlated with the eGFR. In contrast, the campesterol level was positively correlated with the ACR and inversely with the eGFR. In the multiple regression analysis, both lathosterol and campesterol were positively associated with the RemL-C level, independent of the U-ACR, eGFR and other variables. Conclusions: The serum campesterol concentrations are higher in patients with a greater degree of albuminuria and a lower renal funtion. In this study, the markers of cholesterol absorption and synthesis were independent determinants of the RemL-C level. Increased intestinal cholesterol absorption may be an additional mechanism for remnant accumulation in T2DM patients with CKD.

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Section: Subjectsmentioning

confidence: 64%

Kidney Function, Cholesterol Absorption and Remnant Lipoprotein Accumulation in Patients with Diabetes Mellitus

Sonoda

Shoji

Kimoto³

et al. 2014

JAT

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“…This method utilized surfactant and phospholipase D to selectively solubilize and degrade remnant lipoprotein, while the RLP-C assay was an immune separation assay using an immunoaffinity gel containing antiapoA-1 and anti-apoB-100 monoclonal antibodies 22,23) . This RemL-C assay is adaptable for a universal clinical autoanalyzer, supplying quick and high-throughput measurements.…”

Section: Reml-c a Tg-independent Determinant For %Lipidmentioning

confidence: 99%

Impact of Remnant Lipoprotein on Coronary Plaque Components

Matsuo

Matsuoka

Onishi

et al. 2015

JAT

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Aim: Intravascular ultrasound (IVUS) is a useful modality for visualizing atherosclerotic lesions in coronary arteries, not only for the degree of arterial luminal stenosis but also for the plaque composition within the vessel walls. We aimed to determine the relationship between the clinical parameters and coronary plaque characteristics evaluated by IVUS in patients with stable angina under medical treatment. Methods: Plaque measurements within the coronary arteries were collected by coronary angiography and iMAP-IVUS in 40 men with stable angina. The serum remnant-like cholesterol (RemL-C) was measured using homogeneous assays and serum adiponectin and omentin-1 levels were measured by enzyme-linked immunosorbent assays.

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“…These remnant particles (CM-remnants and VLDL-remnants) directly infiltrate the vascular wall, subsequently triggering the development of atherosclerotic disease via accelerated macrophage foam cell formation, platelet coagulation and small dense LDL accumulation, as well as the induction of a low concentration of high-density lipoprotein (HDL) cholesterol (HDL-C) 5) . A number of remnant cholesterol assays have been developed and are currently being used to evaluate the risks of atherosclerotic diseases, such as cardiovascular disease (CAD) [6][7][8] . However, these methods cannot be used to accurately discriminate small intestine-derived CM-remnants from liver-derived VLDLremnants; therefore, the development of a new assay system is required in order to quantitatively measure the CM-remnant concentration independently.…”

Section: Measurementsmentioning

confidence: 99%

Reference Interval for the Apolipoprotein B-48 Concentration in Healthy Japanese Individuals

Masuda

Nishida

Arai

et al. 2014

J Atheroscler Thromb

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Aim: Small intestine-derived chylomicrons and chylomicron remnants, which are predominant in patients with postprandial hypertriglyceridemia, chylomicron syndrome and/or familial dyslipidemia, carry one molecule of apolipoprotein B-48 (apo B-48) per lipoprotein particle. We investigated the reference interval for the apo B-48 concentration. Methods: We studied 516 individuals who provided written informed consent and confirmed that they were not taking any medications. BMI, waist circumference, blood pressure and the fasting serum concentrations of LDL-C, triglyceride (TG), HDL-C and apo B-48 were measured. The Apo B-48 concentrations were compared according to sex, a pre-or postmenopausal status, dyslipidemia (LDL-C ≥ 140 mg/ dL, TG ≥ 150 mg/dL, HDL-C ＜40 mg/dL), metabolic syndrome (MetS) and the number of risk factors. Results: The fasting apo B-48 concentrations (mean±SD) were significantly higher in men than in women (3.8±3.3 μg/mL vs 2.4±1.9 μg/mL, p＜0.001), subjects with a BMI of ≥ 25 kg/m 2 versus a BMI of ＜25 kg/m 2 (4.4±3.7 μg/mL vs 2.8±2.4 μg/mL, p＜0.001) and those with versus without MetS (6.5±4.3 μg/mL vs 3.0±2.6 μg/mL, p＜0.001). High apo B-48 concentrations were also observed in correlation with the number of risk factors for the MetS. The upper reference limit of apo B-48 was estimated to be 5.7 μg/mL among the 332 patients with normolipidemia, excluding those exhibiting a mean value above ±2.58 standard deviations (SDs), as the mean and range of mean ±1.96 SD were calculated to be 2.04 μg/mL (reference value) and 0.74 to 5.65 μg/mL (reference interval), respectively. Conclusions: Based on our study of normolipidemic patients, the upper reference limit for the fasting apo B-48 concentration is estimated to be 5.7 μg/mL. J Atheroscler Thromb, 2014; 21:618-627.

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Development of a Homogeneous Assay to Measure Remnant Lipoprotein Cholesterol

Cited by 51 publications

References 23 publications

Kidney Function, Cholesterol Absorption and Remnant Lipoprotein Accumulation in Patients with Diabetes Mellitus

Kidney Function, Cholesterol Absorption and Remnant Lipoprotein Accumulation in Patients with Diabetes Mellitus

Impact of Remnant Lipoprotein on Coronary Plaque Components

Reference Interval for the Apolipoprotein B-48 Concentration in Healthy Japanese Individuals

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