Development of a histopathological index for skeletal muscle analysis in Rattus norvegicus (Rodentia: Muridae)

Zazula, Matheus Felipe; Andrade, Bárbara Zanardini de; Boaro, Carolina De Toni; Kirsch, Christian Bergmann; Reginato, Aline; Peretti, Ana Luiza; Brancalhão, Rose Meire Costa; Bertolini, Gladson Ricardo Flôr; Naliwaiko, Katya; Guimarães, Ana Tereza Bittencourt; Ribeiro, Lucinéia de Fátima Chasko

doi:10.1016/j.acthis.2022.151892

Cited by 3 publications

(1 citation statement)

References 60 publications

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“…For the characterization of the structural alterations of the muscular tissue, ve patterns of reaction (Zazula et al 2022) were used. Based on the previously described reaction patterns, the slides were evaluated blindly, classifying the reaction patterns and their extensions in the sampling areas.…”

Section: Histopathological Index Analysismentioning

confidence: 99%

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

Zazula

Marca

Oliveira

et al. 2023

Preprint

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Introduction: Glyphosate is an organophosphate herbicide most used in Latin America, with multisystemic effects, including the respiratory system. In this sense, the objective of this research was to analyze the muscle fibers and neuromuscular junctions (JNMs) of the diaphragm muscle of adult mice exposed to the glyphosate-based herbicide in the peripubertal period. Methods: Twelve male mice were used, divided into a control group (CTL, n=6), which received water and a glyphosate-based herbicide group (GBH, n=6), which received 50 mg/Kg/day of Roundup®, both by gavage from 30 to 60 days. At 150 days, the animals were euthanized and the diaphragm was collected for analysis of the muscle fibers through hematoxylin-eosin, Masson's trichrome and Picrossirius Red and the JNMs through the nonspecific esterases reaction. Results: Rounded fibers, hypereosinophilic sarcoplasm and enlarged nuclei were found predominantly in GBH. A reduction in body weight, an increase in muscle fiber morphometry, an increase in type III collagen, a decrease in the overlapping of type I and III collagen, and an increase in the area and greater diameter of the JNMs in GBH were observed. Conclusion: Peripubertal exposure to glyphosate-based herbicides showed morphological changes characteristic of muscle degeneration and altered the morphometry of muscle fibers and JNMs of the diaphragm of adult mice.

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Section: Histopathological Index Analysismentioning

confidence: 99%

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

Zazula

Marca

Oliveira

et al. 2023

Preprint

Self Cite

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Photobiomodulation therapy on skeletal muscles exposed to diabetes mellitus: a systematic review of animal studies

dos Santos Soares,

de Souza Pinto,

Kruger

et al. 2023

Lasers Med Sci

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Maternal periodontitis potentiates monosodium glutamate‐obesity damage on Wistar offspring's fast‐glycolytic muscle

Costa,

de Paula,

Zazula

et al. 2024

Oral Diseases

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ObjectiveTo evaluate the effects of magnifying the damage caused by obesity induced by monosodium glutamate, using a model of maternal periodontitis, on the structure of the anterior tibialis muscle of the offspring.Materials and MethodsTwenty‐four female Wistar rats were divided into four experimental groups: control (n = 6), obese (n = 6), control with periodontitis (n = 6) and obese with periodontitis (n = 6). At 78 days of life, the rats were mated with males without any experimental intervention. The offspring of these rats (n = 1/L), at 120 days of life, were weighed and measured, then euthanized. Plasma was collected for analysis of cytokines IL‐6, IL‐10, IL‐17 and TNF‐α. Adipose tissues were collected and weighed, and the anterior tibial muscle was designated for histomorphological analyses (n = 6/group).ResultsMonosodium glutamate offspring showed significant muscle changes, such as a reduction in the size of fibres and neuromuscular junctions, and an increase in the nucleus and capillaries. However, all these changes were more expressed in monosodium glutamate‐obese with periodontitis offspring.ConclusionThis leads us to suggest a magnifying effect promoted by periodontitis to the damage already well described by monosodium glutamate‐obesity, determined by low‐intensity inflammation, causing greater muscle damage.

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Development of a histopathological index for skeletal muscle analysis in Rattus norvegicus (Rodentia: Muridae)

Cited by 3 publications

References 60 publications

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

Photobiomodulation therapy on skeletal muscles exposed to diabetes mellitus: a systematic review of animal studies

Maternal periodontitis potentiates monosodium glutamate‐obesity damage on Wistar offspring's fast‐glycolytic muscle

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