Development of a highly sensitive ELISA and immunochromatographic strip to detect pentachlorophenol

Abstract

A sensitive monoclonal antibody for targeting pentachlorophenol was prepared with an immunization procedure and cell fusion. The antibody was characterized with an indirect competitive enzymelinked immunosorbent assay (ELISA), which had an IC50 of 0.2 ng/ ml for pentachlorophenol under optimized conditions. The crossreactivity of the ELISA to related compounds was very low. The recovery of pentachlorophenol was 99.2-105% in water samples and 89-110.9% in fish samples. A simple, rapid, and sensitive immunochromatographic strip was produced based on the monoclonal antibody, with a qualitative visual limit of detection of 2 ng/ml in water samples and 10 ng/ml in fish samples, indicating its potential utility in monitoring pentachlorophenol in the field.

Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody

“…The processes of cell fusion and hybridoma screening were performed with methods commonly used in our laboratory (Chen et al, 2015;Song et al, 2016;C. Sun, Liu, Song, Kuang, & Xu, 2016).…”

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

“…To overcome the aforementioned drawbacks, in recent years, strip biosensors have received considerable attention due to their portability, rapid assay times, cost-effectiveness, and ease of use, and especially for their excellent repeatability (Kong, Xie, Liu, Song, & Kuang, 2017;Sun, Liu, Song, Kuang, & Xu, 2016;Yu, Liu, Song, Kuang, & Xu, 2016). In order to detect BPA, an immunochromatographic lateral flow strip was developed , which was directly applied in the analysis of real spiked water samples.…”

Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

“…Other parameters were evaluated based on the optimum condition of coating antigen and primary antibody working point. As previously described (Sun, Liu, Song, Kuang, & Xu, 2016), the ic-ELISA procedure was adapted to adequate anti-HES mAb's assays. Briefly, each well of a Microtiter plate was coated with 100 µl of DES-1C-OVA solution prepared in bicarbonate buffer (0.05 M, pH 9.6).…”