Development of a high throughput 96-well plate sample preparation method for the determination of trileptal (oxcarbazepine) and its metabolites in human plasma

Souppart, C.; M, Decherf; Humbert, Henri; Maurer, Gerd

doi:10.1016/s0378-4347(01)00289-4

Cited by 28 publications

(20 citation statements)

References 6 publications

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“…Over the years, several chromatographic methods for OXC and racemic licarbazepine determination have been developed (Noirfalise and Collinge, 1983;Menge et al, 1987;Elyas et al, 1990;Rouan et al, 1994;Pienimaki et al, 1995;Souppart et al, 2001;Levert et al, 2002;Mandrioli et al, 2003;Franceschi and Furlanut, 2005;Lanckmans et al, 2006). However, only three chiral HPLC assays have been validated to determine S-and R-LC enantiomers, in which a very expensive column and mobile phase were employed (Flesch et al, 1992;Pichini et al, 1995;Volosov et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Enantioselective HPLC‐UV method for determination of eslicarbazepine acetate (BIA 2‐093) and its metabolites in human plasma

Alves

Figueiredo

Castel‐Branco

et al. 2007

Biomedical Chromatography

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Determination of eslicarbazepine acetate ABSTRACT: Eslicarbazepine acetate (BIA 2-093) is a novel central nervous system drug undergoing clinical phase III trials for epilepsy and phase II trials for bipolar disorder. A simple and reliable chiral reversed-phase HPLC-UV method was developed and validated for the simultaneous determination of eslicarbazepine acetate, oxcarbazepine, S-licarbazepine and R-licarbazepine in human plasma. The analytes and internal standard were extracted from plasma by a solid-phase extraction using Waters Oasis ® HLB cartridges. Chromatographic separation was achieved by isocratic elution with water-methanol (88:12, v/v), at a flow rate of 0.7 mL/min, on a LichroCART 250-4 ChiraDex (β-cyclodextrin, 5 μm) column at 30°C. All compounds were detected at 225 nm. Calibration curves were linear over the range 0.4 -8 μg/mL for eslicarbazepine acetate and oxcarbazepine, and 0.4 -80 μg/ mL for each licarbazepine enantiomer. The overall intra-and interday precision and accuracy did not exceed 15%. Mean relative recoveries varied from 94.00 to 102.23% and the limit of quantification of the assay was 0.4 μg/mL for all compounds. This method seems to be a useful tool for clinical research and therapeutic drug monitoring of eslicarbazepine acetate and its metabolites S-licarbazepine, R-licarbazepine and oxcarbazepine.

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Section: Introductionmentioning

confidence: 99%

Enantioselective HPLC‐UV method for determination of eslicarbazepine acetate (BIA 2‐093) and its metabolites in human plasma

Alves

Figueiredo

Castel‐Branco

et al. 2007

Biomedical Chromatography

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“…By this method OXC and its metabolites are quantified accurately in less than 3 min and this aspect is very important for a routine therapeutic monitoring of these drugs. Both sample preparation and analytical run time of the OXC method described in this paper are faster and more simple than other chromatographic methods, using UV or MS as detectors, previously reported [10][11][12][13][14][15][16]. Moreover, because just 10 L are injected into LC/MS/MS system, this method allows us to work with small amounts of biological material, including that coming from experimental animals.…”

Section: Resultsmentioning

confidence: 94%

“…This method requires a simple preparative step with a total chromatographic run time of 3 min, so it is less time consuming compared with other LC/MS and LC/MS/MS methods reported in literature [10][11][12][13][14][15][16]19,20].…”

Section: Resultsmentioning

confidence: 99%

“…To date, some analytical methods reported for OXC and its metabolites determination have been performed using mainly LC or LC/MS procedures. It is well known that these methods are less specific than LC/MS/MS methods and also are time consuming because of long chromatographic analysis and/or complex sample preparation procedures [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a LC/MS/MS method for simultaneous quantification of oxcarbazepine and its main metabolites in human serum

Paglia

D’Apolito

Garofalo

et al. 2007

Journal of Chromatography B

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“…Several analytical methods for the OXCBZ determina-tion in plasma or serum have been published and most of them are based on HPLC procedures (Kumps, 1984;Menge et al, 1987;Pienimaki et al, 1995;Levert et al, 2002aLevert et al, , 2002bSouppart et al, 2001) with UV detection. As OXCBZ, like carbamazepine, exhibits thermal instability, HPLC procedures are preferable over gas chromatography, which in addition requires elaborate sample pretreatment Paar, 1985, 1986).…”

Section: Introductionmentioning

confidence: 99%

Quantitation of oxcarbazepine and its metabolites in human plasma by micellar electrokinetic chromatography

Pucci¹,

Kenndler²,

Raggi³

2003

Biomedical Chromatography

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A reliable micellar electrokinetic chromatographic method for the determination of oxcarbazepine and its two main metabolites, 10-hydroxycarbamazepine and 10,11-trans-dihydroxy-10,11-dihydroxycarbamazepine, in human plasma was developed. The separation and determination of the analytes was achieved using a system consisting of 60 mM SDS in phosphate buffer (30 mM, pH 8.0), to which 20% (v/v) methanol was added. Separation was carried out in an uncoated fused-silica capillary with a separation voltage of 25 kV and currents typically less than 40 microA. Spectrophotometric detection was at 205 nm. Isolation of oxcarbazepine and its metabolites from plasma was accomplished by a solid-phase extraction procedure. The mean extraction yield of the analytes from plasma was higher than 94%. The linear correlation coefficients were better than 0.994 for all analytes. The limit of detection was 0.05 microg/mL, the limit of quantitation 0.15 microg/mL. The repeatability for the spiked blank plasma samples was lower than 1.9% and the intermediate precision lower than 2.1%, both expressed as RSD%. The results obtained analysing real plasma samples from epileptic patients under therapy with Tolep were satisfactory in terms of precision, accuracy and detectability.

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Development of a high throughput 96-well plate sample preparation method for the determination of trileptal (oxcarbazepine) and its metabolites in human plasma

Cited by 28 publications

References 6 publications

Enantioselective HPLC‐UV method for determination of eslicarbazepine acetate (BIA 2‐093) and its metabolites in human plasma

Enantioselective HPLC‐UV method for determination of eslicarbazepine acetate (BIA 2‐093) and its metabolites in human plasma

Development and validation of a LC/MS/MS method for simultaneous quantification of oxcarbazepine and its main metabolites in human serum

Quantitation of oxcarbazepine and its metabolites in human plasma by micellar electrokinetic chromatography

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