Development of a high-throughput differential mobility separation–tandem mass spectrometry (DMS-MS/MS) method for clinical urine drug testing

Hooshfar, Shirin; Tchu, Simone; Yun, Cassandra; Lynch, Kara L.

doi:10.1016/j.jmsacl.2021.12.008

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…A sensitive DMS-MS/MS method was developed and validated for simultaneous identification of 33 drugs and metabolites in human urine samples provided better analytical performance than immunoassay. 14 Furthermore, DMS has been implemented to compensate for liquid chromatography selectivity loss in trap-elute approaches and open port probe sampling interface for high-throughput analysis. Gómez-Ríos et al reported a fast quantitation of opioids isomers in human plasma using biocompatible solid-phase micro extraction (bio-SPME) and an open-port probe sampling interface 15 .…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Liquid Chromatography- Vacuum Differential Mobility Spectrometry-Mass Spectrometry for the Analysis of Isomeric Drugs of Abuse in Human Urine

Cifuentes Girard,

Knight,

Hopfgartner

2024

Preprint

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The use of differential mobility spectrometry at low pressure coupled to liquid chromatography-mass spectrometry (LC-vDMS-MS) was investigated for the analysis of thirteen drugs of abuse (DoA) including: cocaine, ecgonine methyl ester, cocaethylene, benzoylecgonine, norcocaine, tramadol, isomeric pairs of metabolites; O-desmethyl-cis-tramadol and N-desmethyl-cis-tramadol, and cannabinoids; Δ9-tetrahydrocannabinol, Δ9-tetrahydrocannabidiol, 11-hydroxy-Δ9-tetrahydrocannabinol, 11-nor-9carboxy-Δ9-tetrahydrocannabinol, 11-nor-9carboxy-Δ9-tetrahydrocannabinol glucuronide. Different parameters were optimized for isomeric separation, such as LC mobile phase composition (20-100% methanol acetonitrile and isopropanol, flow rate: 8-100 µL/min) and DMS separation voltage. Methanol and acetonitrile significantly affected the compensation voltage of the analytes and improved DMS separation. A short trap/elute LC-vDMS-SIM/MS screening method of 1 min was developed to quantify 11 drugs of abuse (except THC/CBD), in addition to a 4 min LC-vDMS-SIM/MS method to identify and quantify 5 cannabinoids including the isomers THC/CBD and three THC metabolites. The signal responses were linear over a concentration range of 0.005-10 µg/ml for the DoA and 1-1000 ng/ml for cannabinoids. The intra- and inter-day precision were better than 12.2% and accuracy better than 115%. Urine samples from subjects who tested positive for THC and/or cocaine during roadside drug testing were evaluated to assess the performance of the methods LC-vDMS-SIM/MS and LC-MRM/MS. Results show that the developed LC-vDMS-SIM/MS method presents similar performance to LC-MRM/MS with improved sample throughput.

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Section: Introductionmentioning

confidence: 99%

High-Throughput Liquid Chromatography- Vacuum Differential Mobility Spectrometry-Mass Spectrometry for the Analysis of Isomeric Drugs of Abuse in Human Urine

Cifuentes Girard,

Knight,

Hopfgartner

2024

Preprint

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“…Ion mobility is an emerging technique in clinical laboratories that separates gas phase ions based on their mobility in the presence of a buffer gas and an electric field; therefore, the mechanism of separation is charge, size, and shape [10] , [11] , [12] , [13] . As a result, ion mobility has the potential to complement established LC-MS/MS techniques and further enhance the specificity of measurements for technically challenging analytes, such as steroids.…”

Section: Introductionmentioning

confidence: 99%

Improving LC-MS/MS measurements of steroids with differential mobility spectrometry

Chai,

Grebe,

Maus

2023

Journal of Mass Spectrometry and Advances in the Clinical Lab

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“…K L is the ion mobility coefficient at a low electric field. The unit for E / N is Townsend (Td). , Owing to the difference in the mobility of ions at a high and low electric field, the analyte ions can be isolated by applying a compensation voltage (CV) to compensate different targeted ions back to the center area of DMS channel and then detecting them by mass spectrometry. , Since the trajectory of ions in DMS can be adjusted by CV, DMS is more compatible with MS/MS for better matches of the time scale which exhibits significant advantages for targeted and high-throughput applications. − In addition, DMS allows for ion filtration and removal of unwanted ions, which improves the confidence of identification such as bottom-up protein analysis …”

Section: Introductionmentioning

confidence: 99%

CaptiveSpray Differential Ion Mobility Spectrometry Device with Enhanced Ion Transmission and Improved Resolving Power

Zhang

Chen

Wong

et al. 2023

J. Am. Soc. Mass Spectrom.

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A performance enhanced CaptiveSpray differential ion mobility device was designed and constructed by incorporating a circular channel and a gas flow homogenizing channel (GFHC) between the CaptiveSpray ion source and planar differential ion mobility spectrometry (DMS). The GFHC was used to reduce gas flow heterogeneity prior to the entrance of the DMS device. The optimal flared entrance greatly reduces gas flow velocity at the inlet region owing to its relatively large gas inlet interface, which assists in reducing disparities between the minimum and maximum gas velocity along the x-axis. The circular electrode was machined with channels along the x-and y-axis for the passage of auxiliary gas and was applied with a potential to focus the incoming ions from the CaptiveSpray source into the DMS channel. Using reserpine as a reference standard, substantial signal enhancement was achieved with a concomitant reduction of the peak width in the ionogram.

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Development of a high-throughput differential mobility separation–tandem mass spectrometry (DMS-MS/MS) method for clinical urine drug testing

Cited by 10 publications

References 26 publications

High-Throughput Liquid Chromatography- Vacuum Differential Mobility Spectrometry-Mass Spectrometry for the Analysis of Isomeric Drugs of Abuse in Human Urine

High-Throughput Liquid Chromatography- Vacuum Differential Mobility Spectrometry-Mass Spectrometry for the Analysis of Isomeric Drugs of Abuse in Human Urine

Improving LC-MS/MS measurements of steroids with differential mobility spectrometry

CaptiveSpray Differential Ion Mobility Spectrometry Device with Enhanced Ion Transmission and Improved Resolving Power

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