Development of a High-Throughput Affinity Mass Spectrometry (AMS) Platform Using Laser Diode Thermal Desorption Ionization Coupled to Mass Spectrometry (LDTD-MS)

High-Throughput Affinity Selection Mass Spectrometry Using SAMDI-MS to Identify Small-Molecule Binders of the Human Rhinovirus 3C Protease

“…Another limitation of ASMS is the reagent requirement, with various approaches using 5–10 µM of the target. 6,7,14,35 Conversely, SAMDI-ASMS technology benefits from up to 50-fold less concentration of the analyte, opening avenues to screen previously intractable targets that may suffer from low expression. Lastly, go or no-go decisions rely on the quality of data generated.…”

Recent Developments in Mass Spectrometry to Support Next-Generation Synthesis and Screening

“…Eliminating the liquid handling bottleneck allows sampling times at the subsecond/sample speeds needed for MS to compete with optical methods. A recent study by researchers at Amgen utilized laser diode thermal desorption (LDTD) MS to screen small libraries (∼68,000 compounds) . Although analysis speeds for this method are comparable to those for SPE-MS (∼11 s/sample), LDTD-MS provided comparable data quality with practical advantages that included reduced solvent consumption and reduced carryover.…”

“…A recent study by researchers at Amgen utilized laser diode thermal desorption (LDTD) MS to screen small libraries (∼68,000 compounds). 34 Although analysis speeds for this method are comparable to those for SPE-MS (∼11 s/ sample), LDTD-MS provided comparable data quality with practical advantages that included reduced solvent consumption and reduced carryover. However, at these speeds both LDTD and RapidFire-MS must rely on compound pooling to assess larger compound libraries.…”

Hit Discovery Methodology

“…This brings us to newer approaches, as addressed in the technology review 21 by David McLaren et al An example article by Sahasrabuddhe et al 22 describes how mass spectrometry has established itself in recent years as a screening technology, capable of being applied as both a discrete screening assay and a multiplexed-affinity selection method.…”