Development of a high resolution melting (HRM) marker linked to genic male sterility inCapsicum annuum L.

Abstract

With 4 figures and 1 table Abstract The characteristics of male sterility (MS) are used in breeding programmes to achieve economical seed production. Male sterility is divided into genic male sterility (GMS) and cytoplasmic male sterility (CMS), which are used to breed commercial pepper varieties. The CMS system, however, is not feasible in some pepper varieties, including bell peppers, because of the absence of a restorer source. GMS is thus important for seed production in bell peppers. In this study, a GMS‐linked marker from bell peppers was developed using the bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) method using F2 and sibling individuals. We used 1024 AFLP primer sets and found a polymorphism from EcoRI ACG/MseI GTT among the siblings. An internal sequence‐based primer was designed from the 395 bp sequence for high‐resolution melting (HRM) analysis, and the marker score of 87 of 92 F2 individuals corresponded to their phenotypes. The marker was mapped on chromosome 5 on the AC99 map.

Mapping and validation of markers linked to Rf gene in sweet pepper (Capsicum annuum var grossum)

“…It can be supported by the findings of Jo et al (2010), who explained that although several markers are closely linked to the Rf gene in a given population, most of the markers were unable to correctly detect genotypes for a wider range of pepper lines implying that rearrangements may have occurred in the Rf locus. Similarly, several DNA markers distinguishing Rf and rf alleles have been reported in chili peppers (Pakozdi et al, 2002;Kumar et al, 2007;Lee et al, 2008a;Min et al, 2009;Jo et al, 2010;Lee et al, 2012 andMol et al, 2020). But these markers closely linked to the Rf locus were often inconsistent with the phenotypes in inbred lines (Zhang et al, 2000;Kumar et al, 2007).…”

A 163‐bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)

“…In pepper, to date, more than 20 genes controlling GMS have been found, and meanwhile, several of them have been successfully physically mapped. To give more detail, the ms3 and ms10 have been mapped on chromosome 1 (Aulakh et al., 2016; Naresh et al., 2018), the msc‐1 has been located on chromosome 2 (Cheng et al., 2018), the ms‐8 was found on chromosome 4 (Bartoszewski et al., 2012), and the three GMS genes including ms1 (allelic with msc‐2 ), ms w and GMS gene from NW1 were both mapped on chromosome 5 (Cheng, Li, et al., 2020; Jeong et al., 2018; Lee et al., 2012; Naresh et al., 2018). However, the rest of the other documented pepper GMS genes are still not physically mapped.…”

“…To date, a total of more than 20 GMS mutants, including ms1 to ms4 , ms6 to ms15 , msc‐1 , msc‐2 (Wang & Bosland, 2006), msk (Lee et al., 2010; Shifriss, 1973), ms w (Naresh et al., 2018), the GMS mutant from NW1 bell pepper (Lee et al., 2012) and the GMS mutant developed by interspecific hybridization and then by chemical mutagenesis (Huang et al., 2015), are documented in pepper. The majority of the above‐mentioned pepper GMS mutants are controlled by single recessive genes.…”

QTL analysis and development of HRM markers associated with fruit shape in interspecific pears (Pyrus pyrifolia × P. bretschneideri)

“…HRM markers have been developed based on SNPs associated with a trait of interest. For instance, Lee et al (2012) developed HRM markers linked to genetic male sterility in pepper. Wang et al (2016) distinguished dwarf and standard growth types of pears through HRM analysis based on SNPs associated with the PcDw.…”