Development of a High- Performance Liquid Chromatography with Diode- Array Detection Method using Monolithic Column for Simultaneous Determination of Five Tetracyclines Residues in Fish Muscles

Ghorbel–Abid, Ibtissem; Belhassen, Habibi

doi:10.4172/2324-9323.1000209

Cited by 6 publications

(1 citation statement)

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“…These methods include spectrophotometry [7], liquid chromategraphy [8] and gas chromatography [9], whereas lidocaine HCl analytical methods in the literature include spectrophotometry [10,11], electrochemical methods [12,13], HPLC [14,15], and GLC [16]. Oxytetracycline HCl literature survey revealed several methods including spectrophotometry [17], HPLC [18,19], and electrochemical methods [20,21]. To the best of our knowledge, up to date, no simple spectrophotometric method has been described in the literature for the determination of the three studied drugs simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Critical assessment of smart calculation-based spectroscopy versus chemometric-assisted methods: Application to combined antibiotic formulations

et al. 2022

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This work describes a comparative study of two multivariate chemometric and univariate spectrophotometric methods for the determination of a ternary drug mixture containing oxytetracycline HCl, bromhexine HCl, and lidocaine HCl. All methods show high sensitivity and similar linearity range. Meanwhile, the chemometric method has the advantage of higher accuracy, higher specificity and better regression parameters. The two spectrophotometric methods are constant multiplication coupled with spectrum subtraction and successive ratio subtraction coupled with spectrum subtraction while the chemometric method used partial least square and principal component regression models. In addition, a spiking technique was used to increase the concentration of bromhexine HCl in the dosage form, allowing its determination despite its low contribution. Methods were successfully applied in the dosage form Oxyclear® veterinary injection in pure powder as well as in its pharmaceutical formulation. Statistical comparison showed no significant difference between the developed methods and the reference method.

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Section: Introductionmentioning

confidence: 99%

Critical assessment of smart calculation-based spectroscopy versus chemometric-assisted methods: Application to combined antibiotic formulations

et al. 2022

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Green validated chromatographic methods for simultaneous determination of co-formulated oxytetracycline HCl and flunixin meglumine in the presence of their impurities in different matrices

et al. 2022

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Green analytical chemistry is an important area of chemical sciences. So, developing competent eco-friendly analytical tools is a big challenge. This study devotes two chromatographic techniques for the simultaneous determination of a binary formulated mixture of oxytetracycline HCl (OXY) and flunixin meglumine (FLU), and their impurities tetracycline (TRC) and 2-chloronicotinic acid (CNA), respectively. Primarily, a TLC densitometric method is proposed and validated using TLC plates sprayed with 5% EDTA of pH 9 as a stationary phase and (acetonitrile/ distilled water/ ethanol 7:2:1, by volume) as a developing system. Well-separated spots are detected at 267 nm where linear relations have been achieved at 0.05–2 μg/band and 0.01–2 μg/band for OXY and FLU, respectively. The second developed and validated method is HPLC which is fulfilled on Hypersil BDS column-C18. A mobile phase of distilled water with 0.1%TFA/ acetonitrile in a ratio of 82: 18, v/v firstly then 70: 30, v/v after 9 min is used in a sequential isocratic elution at 210 nm for FLU and 267 nm for OXY, TRC, and CNA. Sensitive and wide linearity ranges are achieved at 0.05–200 μg/mL for both drugs. The two methods are applied successfully in cattle meat and milk for cited drugs determination. According to ICH guidelines, a validation study has been accomplished for the proposed methods. Statistical comparison has been carried out with official and reported methods. Eventually, the greenness of both procedures is evaluated using Eco-Scale which gives eco-friendly results. Graphical abstract

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Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study

Mishra

Jyotshna

Singh

et al. 2019

International Journal of Biological Macromolecules

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Development of a High- Performance Liquid Chromatography with Diode- Array Detection Method using Monolithic Column for Simultaneous Determination of Five Tetracyclines Residues in Fish Muscles

Cited by 6 publications

References 0 publications

Critical assessment of smart calculation-based spectroscopy versus chemometric-assisted methods: Application to combined antibiotic formulations

Critical assessment of smart calculation-based spectroscopy versus chemometric-assisted methods: Application to combined antibiotic formulations

Green validated chromatographic methods for simultaneous determination of co-formulated oxytetracycline HCl and flunixin meglumine in the presence of their impurities in different matrices

Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study

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