Development of a High Performance Micro-Spectrophotometer for Biochemical Analyzer

Abstract

To overcome the drawbacks of prism and concave gratings commonly used as dispersive devices in the traditional spectrophotometer, including low splitting efficiency, low resolution and little luminous flux, a high performance micro-spectrophotometer for biochemical analyzer (BCA) based on charge coupled devices (CCD) and virtual instrument technique is successfully developed, in which a plane grating is employed as the dispersive device, a linear CCD and a high speed peripheral component interconnect (PCI) bus data acquisition card are combined as the spectral detection system. Meanwhile, a custom-built Czerny-Turner (CT) typed monochrometer with a symmetric crossed structure is designed as the light splitting apparatus in this spectrophotometer. The experimental results demonstrate that this spectrophotometer realizes the capacity of detecting absorption intensity larger than 1000 (a.u.) in the ultraviolet and visible wavebands, the wavelength repeatability is less than 0.5 nm, the stray-light is less than 0.5% at the wavelength of 360 nm, its spectral accuracy is less than ± 0.5 nm and its spectral measurement range and resolution reaches 300~800 nm and 1 nm, respectively. In addition, this system has the characteristics of post light-splitting and multi-channel parallel analysis, etc. This spectrophotometer for BCA has the potential value by virtue of optimized imaging system, high accuracy and low cost, etc.

A Small Automated Enzyme Immunoassay Analyzer System Design and Implementation Based on Immunoenzymatic Techniques

“…Finally substrates added immune enzyme catalyzes show the color. Qualitative and quantitative determination results are judged by the human eye or spectrophotometer [2]. This technology referred to as ELIEA (enzyme linked immunosorbent assay).…”

“…Small automatic enzyme immunoassay reaction tester test methods. At present, the experiment used enzyme-linked immunosorbent assay technique has three kinds, is divided into: indirect method and double antibody sandwich method, competition law [2]. Test methods adopted in this research is the indirect method principle.…”

“…The photoelectric detection mechanism is the core part of biological analysis instrument and the actuator tested [4]. Based on Longbow Bill's law [2], the traditional photoelectric detection device is evolved on the basis of spectrophotometer. In order to achieve the miniaturization of instrument, this study reaction tester design model of optical detection system based on spectral analyzer, which composed of light source, optical system and micro spectrometer [5], the core principle of the spectrum analyzer is composite light source projection to the holographic concave grating to split light, photoelectric coupling element detector (linear CCD)that has the advantages that each pixel element is very small and the pixel element distance is also very small between each pixel, so that has the high detection precision and sensitivity.…”