Development of a herbal mouthwash containing a mixture of essential oils and plant extracts and<i>in vitro</i>testing of its antimicrobial efficiency against the planktonic and biofilm-enclosed cariogenic bacterium<i>Streptococcus mutans</i>

Dimou, Ioanna; Dritsas, Stavros; Aggelopoulou, Paraskevi; Vassilatou, Katerina; Damianaki, Sofia; Giaouris, Efstathios

doi:10.1080/08927014.2021.1924693

Cited by 8 publications

(4 citation statements)

References 75 publications

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“…The MBIC of each antimicrobial agent (BAC, ERY, and LA) against each of the eight Campylobacter isolates (CAMP_005/022/025/048/083/091/114/130) that were able to form biofilms under monoculture conditions (preliminary experiments; data not presented) was determined using the CV staining assay as previously described [ 76 ]. For this, bacteria were left to form biofilms on 96-well PS microtiter plates (transparent, flat, Cat.…”

Section: Methodsmentioning

confidence: 99%

Comparative Assessment of the Antibacterial and Antibiofilm Actions of Benzalkonium Chloride, Erythromycin, and L(+)-Lactic Acid against Raw Chicken Meat Campylobacter spp. Isolates

Kostoglou,

Vass,

Giaouris

2024

Antibiotics

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Campylobacter spp. are significant zoonotic agents, which cause annually millions of human cases of foodborne gastroenteritis worldwide. Their inclusion in biofilms on abiotic surfaces seems to play a pivotal role in their survival outside of the host, growth, and spread. To successfully mitigate the risks that arise with these bacteria, it is crucial to decrease their prevalence within the food production chain (from farm to the table), alongside the successful treatment of the resulting illness, known as campylobacteriosis. For this, the use of various antimicrobial agents remains actively in the foreground. A general-purpose biocide and cationic surfactant (benzalkonium chloride; BAC), a widely used macrolide antibiotic (erythromycin; ERY), and a naturally occurring organic acid (L(+)-lactic acid; LA) were comparatively evaluated in this work for their potential to inhibit both the planktonic and biofilm growth of 12 selected Campylobacter spp. (of which, seven were C. jejuni and five were C. coli) raw chicken meat isolates, all grown in vitro as monocultures. The inhibitory action of LA was also studied against four mixed-culture Campylobacter biofilms (each composed of three different isolates). The results showed that the individual effectiveness of the agents varied significantly depending on the isolate, growth mode (planktonic, biofilm), intercellular interactions (monocultures, mixed cultures), and the growth medium used (with special focus on blood presence). Thus, BAC exhibited minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and minimum biofilm inhibitory concentrations (MBICs) that ranged from 0.5 to 16 μg/mL. Interestingly enough, these values varied widely from 0.25 to 1024 μg/mL for ERY. Concerning LA, the MICs, MBCs, and MBICs varied from 1024 to 4096 μg/mL, with mixed-culture biofilm formation always being more difficult to suppress when compared to biofilm monocultures. In addition, it was evident that intercellular interactions encountered within mixed-culture Campylobacter biofilms significantly influenced both the population dynamics and the tolerance of each consortium member to acid exposure. Overall, the findings of this study provide useful information on the comparative effectiveness of three well-known antimicrobial agents for the control of Campylobacter spp. under various growth modes (i.e., planktonic, biofilm, monocultures, mixed cultures) that could potentially be encountered in food production and clinical settings.

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Section: Methodsmentioning

confidence: 99%

Comparative Assessment of the Antibacterial and Antibiofilm Actions of Benzalkonium Chloride, Erythromycin, and L(+)-Lactic Acid against Raw Chicken Meat Campylobacter spp. Isolates

Kostoglou,

Vass,

Giaouris

2024

Antibiotics

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“…The MBIC of the EO against the biofilm growth of each strain was determined by the CV staining assay as previously described [ 85 ]. For this, bacteria were initially left to form biofilms on 96-well PS microtiter plates (transparent, flat, Cat.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition, Antioxidant, and Antibiofilm Properties of Essential Oil from Thymus capitatus Plants Organically Cultured on the Greek Island of Lemnos

Maniki

Kostoglou

Paterakis³

et al. 2023

Molecules

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Essential oils (EOs) are mixtures of volatile plant secondary metabolites and have been exploited by humans for thousands of years for various purposes because of their many bioactivities. In this study, the EO from Thymus capitatus, a thyme species organically cultured on the Greek Island of Lemnos, was analyzed for its chemical composition (through GC-FID and GC-MS), antioxidant activity (AA), and total phenolic content (TPC), as well as its antimicrobial and antibiofilm actions against three important foodborne bacterial pathogens (Salmonella enterica ser. Typhimurium, Listeria monocytogenes, and Yersinia enterocolitica). For the latter investigations, the minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentrations (MBICs) of the EO against the planktonic and biofilm growth of each pathogen were determined, together with the minimum biofilm eradication concentrations (MBECs). Results revealed that T. capitatus EO was rich in thymol, p-cymene, and carvacrol, presenting high AA and TPC (144.66 μmol TroloxTM equivalents and 231.32 mg gallic acid equivalents per g of EO, respectively), while its MICs and MBICs ranged from 0.03% to 0.06% v/v and 0.03% to 0.13% v/v, respectively, depending on the target pathogen. The EO was able to fully destroy preformed (mature) biofilms of all three pathogenic species upon application for 15 min, with MBECs ranging from 2.00 to 6.25% v/v. Overall, the results demonstrate that the EO of organically cultured T. capitatus presents strong antioxidant, antibacterial, and antibiofilm properties and could, therefore, be further exploited as a functional and antimicrobial natural formulation for food and health applications.

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“…The MBIC of LA against the biofilm growth of each Campylobacter isolate was determined using the crystal violet (CV) staining assay as previously described [20]. For this, bacteria were initially left to form biofilms on 96-well PS microtiter plates (transparent, flat, Cat.…”

Section: Determination Of Mbics Of La Against Single Culturesmentioning

confidence: 99%

Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures

Kostoglou,

Vass,

Giaouris

2023

Foods 2023

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Development of a herbal mouthwash containing a mixture of essential oils and plant extracts andin vitrotesting of its antimicrobial efficiency against the planktonic and biofilm-enclosed cariogenic bacteriumStreptococcus mutans

Cited by 8 publications

References 75 publications

Comparative Assessment of the Antibacterial and Antibiofilm Actions of Benzalkonium Chloride, Erythromycin, and L(+)-Lactic Acid against Raw Chicken Meat Campylobacter spp. Isolates

Comparative Assessment of the Antibacterial and Antibiofilm Actions of Benzalkonium Chloride, Erythromycin, and L(+)-Lactic Acid against Raw Chicken Meat Campylobacter spp. Isolates

Chemical Composition, Antioxidant, and Antibiofilm Properties of Essential Oil from Thymus capitatus Plants Organically Cultured on the Greek Island of Lemnos

Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures

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