Development of a handheld fluorescence imaging camera for intraoperative sentinel lymph node mapping

Szyc, Łukasz; Bonifer, Stefanie; Walter, A; Jagemann, Uwe; Grosenick, Dirk; Macdonald, Rainer

doi:10.1117/1.jbo.20.5.051025

Cited by 13 publications

(10 citation statements)

References 49 publications

(66 reference statements)

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“…A different class of tissue-mimicking phantoms focuses on simulating the geometrical features of tissue organs and can be used for clinical training in intraoperative imaging. A lymph-node mimicking phantom was constructed 21 to assess the efficacy of a real-time fluorescence imaging system [ Fig. 2(a)].…”

Section: Shape-maintaining and Organ-mimicking Fluorescent Phantomsmentioning

confidence: 99%

“…Single camera systems visualizing only fluorescence have also been considered for reducing cost and camera weight. 21 Alternative approaches build systems with multiple cameras to visualize at different spectral bands. For example, three camera systems have been employed to collect images from two different NIR channels and one visible channel 5,22 or for collecting information at different spectral regions for correcting fluorescence images for illumination and tissue-absorption variations in real time.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive phantom for interventional fluorescence molecular imaging

et al. 2016

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Fluorescence imaging has been considered for over a half-century as a modality that could assist surgical guidance and visualization. The administration of fluorescent molecules with sensitivity to disease biomarkers and their imaging using a fluorescence camera can outline pathophysiological parameters of tissue invisible to the human eye during operation. The advent of fluorescent agents that target specific cellular responses and molecular pathways of disease has facilitated the intraoperative identification of cancer with improved sensitivity and specificity over nonspecific fluorescent dyes that only outline the vascular system and enhanced permeability effects. With these new abilities come unique requirements for developing phantoms to calibrate imaging systems and algorithms. We briefly review herein progress with fluorescence phantoms employed to validate fluorescence imaging systems and results. We identify current limitations and discuss the level of phantom complexity that may be required for developing a universal strategy for fluorescence imaging calibration. Finally, we present a phantom design that could be used as a tool for interlaboratory system performance evaluation.

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Section: Shape-maintaining and Organ-mimicking Fluorescent Phantomsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive phantom for interventional fluorescence molecular imaging

et al. 2016

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“…Pallota et al manufactured a custom-made system for ICG detection of lymphatic vessels in flap reconstructed lower extremities to predict lymphedema in these patients [4]. Szyc et al designed a handhold ICG fluorescence imaging system for sentinel node biopsy [5].…”

Section: Discussionmentioning

confidence: 99%

In-House Handmade Camera for Indocyanine Green Video Angiography

Azarhomayoun

Nouri

2018

IrJNS

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Background and Aim: Vascular imaging during surgical procedures is very important and has many applications. There are several methods for intraoperative vascular assessment such as intraoperative angiography, Doppler, and fluorescence-based techniques. The latter group and specially the Indocyanine Green (ICG) Video Angiography (VA) is commonly used for vascular surgery and sentinel node biopsy. However, the cost of these microscope mounted cameras limit their availability in the developing countries. Considering this limitation, we designed and constructed a simplified low-cost camera for real-time ICG angiography. In this article, we describe the device structure and give a preliminary report on its usage in an animal model. Methods and Materials/ Patients: ICG-VA camera was designed and constructed in our laboratory. The device consists of optic filters, light sources, and cameras. Results: After inducing anesthesia and exposing mesenteric vessels in a mouse, ICG-VA of these vessels were taken and recorded. Conclusion: Considering the very low cost of the device and its acceptable image quality, it can be utilized in operation theatres and or laboratories for research purposes.

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“…Thus, new technologies for real-time guidance are needed to address limitations that may lead to incomplete surgical treatment of disease. 15,17,18 Nevertheless, most of these phantoms resolve one or a few parameters and do not allow for comprehensive characterization of all variables associated with fluorescence imaging performance. 4 Fluorescence imaging has the potential to improve surgical and endoscopic guidance and positively impact the clinical management and prognosis of numerous diseases.…”

Section: Introductionmentioning

confidence: 99%

Benchmarking of fluorescence cameras through the use of a composite phantom

et al. 2017

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Fluorescence molecular imaging (FMI) has shown potential to detect and delineate cancer during surgery or diagnostic endoscopy. Recent progress on imaging systems has allowed sensitive detection of fluorescent agents even in video rate mode. However, lack of standardization in fluorescence imaging challenges the clinical application of FMI, since the use of different systems may lead to different results from a given study, even when using the same fluorescent agent. In this work, we investigate the use of a composite fluorescence phantom, employed as an FMI standard, to offer a comprehensive method for validation and standardization of the performance of different imaging systems. To exclude user interaction, all phantom features are automatically extracted from the acquired epi-illumination color and fluorescence images, using appropriately constructed templates. These features are then employed to characterize the performance and compare different cameras to each other. The proposed method could serve as a framework toward the calibration and benchmarking of FMI systems, to facilitate their clinical translation.

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Development of a handheld fluorescence imaging camera for intraoperative sentinel lymph node mapping

Cited by 13 publications

References 49 publications

Comprehensive phantom for interventional fluorescence molecular imaging

Comprehensive phantom for interventional fluorescence molecular imaging

In-House Handmade Camera for Indocyanine Green Video Angiography

Benchmarking of fluorescence cameras through the use of a composite phantom

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