Development of a handheld compression optical coherence elastography probe with a disposable stress sensor

Wang, Xiwen; Wu, Qian; Chen, Jiewen; Mo, Jianhua

doi:10.1364/ol.429955

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Separately, Wang et al . mounted a circular glass window connected to a metal ring through elastic spokes at the tip of a handheld quantitative compression OCE probe [ 14 ]. Then, the applied force was measured as a function of window displacement using OCT.…”

Section: Introductionmentioning

confidence: 99%

In situ stress estimation in quantitative micro-elastography

Navaeipour,

Hepburn,

et al. 2024

Biomed. Opt. Express

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In quantitative micro-elastography (QME), a pre-characterized compliant layer with a known stress-strain curve is utilized to map stress at the sample surface. However, differences in the boundary conditions of the compliant layer when it is mechanically characterized and when it is used in QME experiments lead to inconsistent stress estimation and consequently, inaccurate elasticity measurements. Here, we propose a novel in situ stress estimation method using an optical coherence tomography (OCT)-based uniaxial compression testing system integrated with the QME experimental setup. By combining OCT-measured axial strain with axial stress determined using a load cell in the QME experiments, we can estimate in situ stress for the compliant layer, more accurately considering its boundary conditions. Our proposed method shows improved accuracy, with an error below 10%, compared to 85% using the existing QME technique with no lubrication. Furthermore, demonstrations on hydrogels and cells indicate the potential of this approach for improving the characterization of the micro-scale mechanical properties of cells and their interactions with the surrounding biomaterial, which has potential for application in cell mechanobiology.

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Section: Introductionmentioning

confidence: 99%

In situ stress estimation in quantitative micro-elastography

Navaeipour,

Hepburn,

et al. 2024

Biomed. Opt. Express

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Optical Coherence Elastography Needle for Biomechanical Characterization of Deep Tissue

Mieling,

Latus,

Fischer

et al. 2023

Lecture Notes in Computer Science

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Analysis of strain estimation methods in phase-sensitive compression optical coherence elastography

Pijewska

Fang

et al. 2022

Biomed. Opt. Express

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In compression optical coherence elastography (OCE), deformation is quantified as the local strain at each pixel in the OCT field-of-view. A range of strain estimation methods have been demonstrated, yet it is unclear which method provides the best performance. Here, we analyze the two most prevalent strain estimation methods used in phase-sensitive compression OCE, i.e., weighted least squares (WLS) and the vector method. We introduce a framework to compare strain imaging metrics, incorporating strain sensitivity, strain signal-to-noise ratio (SNR), strain resolution, and strain accuracy. In addition, we propose a new phase unwrapping algorithm in OCE, fast phase unwrapping (FPU), and combine it with WLS, termed WLSFPU. Using the framework, we compare this new strain estimation method with both a current implementation of WLS that incorporates weighted phase unwrapping (WPU), termed WLSWPU, and the vector method. Our analysis reveals that the three methods provide similar strain sensitivity, strain SNR, and strain resolution, but that WLSFPU extends the dynamic range of accurate, measurable local strain, e.g., measuring a strain of 2.5 mɛ with ∼4% error, that is ×11 and ×15 smaller than the error measured using WLSWPU and the vector method, respectively. We also demonstrate, for the first time, the capability to detect sub-resolution contrast in compression OCE, i.e., changes in strain occurring within the strain axial resolution, and how this contrast varies between the different strain estimation methods. Lastly, we compare the performance of the three strain estimation methods on mouse skeletal muscle and human breast tissue and demonstrate that WLSFPU avoids strain imaging artifacts resulting from phase unwrapping errors in WLSWPU and provides improved contrast over the other two methods.

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Development of a handheld compression optical coherence elastography probe with a disposable stress sensor

Cited by 3 publications

References 18 publications

In situ stress estimation in quantitative micro-elastography

In situ stress estimation in quantitative micro-elastography

Optical Coherence Elastography Needle for Biomechanical Characterization of Deep Tissue

Analysis of strain estimation methods in phase-sensitive compression optical coherence elastography

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